In:
The Journal of Physiology, Wiley, Vol. 553, No. 3 ( 2003-12), p. 789-802
Abstract:
Acetylcholine has long been known to excite sympathetic neurons via M 1 muscarinic receptors through an inhibition of M‐currents. Nevertheless, it remained controversial whether activation of muscarinic receptors is also sufficient to trigger noradrenaline release from sympathetic neurons. In primary cultures of rat superior cervical ganglia, the muscarinic agonist oxotremorine M inhibited M‐currents with half‐maximal effects at 1 μ m and induced the release of previously incorporated [ 3 H]noradrenaline with half‐maximal effects at 10 μ m . This latter action was not affected by the nicotinic antagonist mecamylamine which, however, abolished currents through nicotinic receptors elicited by high oxotremorine M concentrations. Ablation of the signalling cascades linked to inhibitory G proteins by pertussis toxin potentiated the release stimulating effect of oxotremorine M, and the half‐maximal concentration required to stimulate noradrenaline release was decreased to 3 μ m . Pirenzepine antagonized the inhibition of M‐currents and the induction of release by oxotremorine M with identical apparent affinity, and both effects were abolished by the muscarinic toxin 7. These results indicate that one muscarinic receptor subtype, namely M 1 , mediates these two effects. Retigabine, which enhances M‐currents, abolished the release induced by oxotremorine M, but left electrically induced release unaltered. Moreover, retigabine shifted the voltage‐dependent activation of M‐currents by about 20 mV to more negative potentials and caused 20 mV hyperpolarisations of the membrane potential. In the absence of retigabine, oxotremorine M depolarised the neurons and elicited action potential discharges in 8 of 23 neurons; in its presence, oxotremorine M still caused equal depolarisations, but always failed to trigger action potentials. Action potential waveforms caused by current injection were not affected by retigabine. These results indicate that the inhibition of M‐currents is the basis for the stimulation of transmitter release from sympathetic neurons via M 1 muscarinic receptors.
Type of Medium:
Online Resource
ISSN:
0022-3751
,
1469-7793
DOI:
10.1113/jphysiol.2003.052449
Language:
English
Publisher:
Wiley
Publication Date:
2003
detail.hit.zdb_id:
1475290-6
SSG:
12
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