In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 60, No. 12 ( 2010-12-01), p. 2972-2978
Abstract:
A novel aerobic, heterotrophic bacterium, designated BiosLi39 T , was isolated from the South East Pacific Ocean. Cells were Gram-negative gliding rods forming yellow colonies on marine agar. The isolate was oxidase-, catalase- and alkaline phosphatase-positive and β -galactosidase-negative. Strain BiosLi39 T grew at 20-37 °C (optimum 30 °C), at pH 7.0–9.0 (optimum pH 8.0) and with 20–60 g NaCl l −1 (optimum 30–50 g NaCl l −1 ). The fatty acids ( 〉 1 %) comprised iso-C 14 : 0 , iso-C 15 : 1 G, iso-C 15 : 0 , anteiso-C 15 : 0 , C 15 : 1 G, C 15 : 0 , iso-C 15 : 0 2-OH, iso-C 16 : 1 G, iso-C 16 : 0 , iso-C 16 : 0 3-OH, iso-C 16 : 0 2-OH, iso-C 17 : 0 3-OH, C 17 : 0 2-OH and three unidentified components with equivalent chain lengths of 17.87, 18.10 and 18.71. A significant proportion of the hydroxylated fatty acids are amide-linked. The lipid pattern indicated the presence of phosphatidylethanolamine, two unidentified aminolipids and three unidentified polar lipids. The strain contained menaquinone 7 as the sole respiratory lipoquinone and did not produce flexirubin-type pigments. The G+C content of the genomic DNA was 37.2 mol%. Comparative 16S rRNA gene sequence analysis indicated that strain BiosLi39 T was distantly related to all of the representatives of the phylum Bacteroidetes . Its closest relative was Marinoscillum furvescens IFO 15994 T , with which it shared 92.5 % 16S rRNA gene sequence similarity. On the basis of genotypic, phenotypic and chemotaxonomic characteristics, we propose a novel genus and species, Ekhidna gen. nov., sp. nov., with type strain BiosLi39 T (=DSM 19307 T =CIP 109600 T =OOB 398 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.018804-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2010
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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