In:
ChemBioChem, Wiley, Vol. 22, No. 7 ( 2021-04-06), p. 1252-1260
Abstract:
Fusobacterium nucleatum is a common member of the oral microbiota. However, this symbiont has been found to play an active role in disease development. As a Gram‐negative bacterium, F. nucleatum has a protective outer membrane layer whose external leaflet is mainly composed of lipopolysaccharides (LPSs). LPSs play a crucial role in the interaction between bacteria and the host immune system. Here, we characterised the structure of the O‐antigen and lipid A from F. nucleatum ssp. animalis ATCC 51191 by using a combination of GC‐MS, MALDI and NMR techniques. The results revealed a novel repeat of the O‐antigen structure of the LPS, [→4)‐β‐ d ‐Glc p NAcA‐(1→4)‐β‐ d ‐Glc p NAc3NAlaA‐(1→3)‐α‐ d ‐Fuc p NAc4NR‐(1→], (R=acetylated 60 %), and a bis‐phosphorylated hexa‐acylated lipid A moiety. Taken together these data showed that F. nucleatum ATCC 51191 has a distinct LPS which might differentially influence recognition by immune cells.
Type of Medium:
Online Resource
ISSN:
1439-4227
,
1439-7633
DOI:
10.1002/cbic.202000751
Language:
English
Publisher:
Wiley
Publication Date:
2021
detail.hit.zdb_id:
2020469-3
SSG:
12
Permalink