In:
The Journal of Immunology, The American Association of Immunologists, Vol. 163, No. 1 ( 1999-07-01), p. 343-350
Abstract:
The multistep model of leukocyte adhesion reveals that selectins mediate rolling interactions and that integrins mediate firm adhesion processes. In this study, the interaction between eosinophils and TNF-α-activated HUVEC (second or third passage) was studied under flow conditions (0.8 and 3.2 dynes/cm2). Especially the role of α4 integrins on eosinophils and E-selectin on HUVEC was studied. Inhibition of the integrin α4 chain on eosinophils reduced the number of firmly adhered resting eosinophils to TNF-α-stimulated endothelium by 43% whereas the percentage rolling cells increased 2.2-fold compared with untreated control eosinophils. Blocking of E-selectin on the endothelium reduced the number of adherent eosinophils by only 23% and 16%. In this situation, however, hardly any rolling adhesion was observed, and the few rolling cells showed a low rolling velocity. Blocking both α4 integrin on eosinophils and E-selectin on HUVEC reduced the number of adhered eosinophils by 95%. P-selectin did not significantly participate in eosinophil adhesion to TNF-α-activated HUVEC. Inhibition of both α4 integrins and β2 integrins on eosinophils resulted in a reduction of adhered cells by 65% and a 3-fold increase in percentage rolling cells. Taken together, these results clearly show that resting eosinophils preferentially use constitutively active α4 integrins (α4β1, α4β7) for the first attachment to TNF-α-activated HUVEC. In addition, α4 integrins and E-selectin work synergistically in eosinophil adherence to TNF-α-activated HUVEC. Although E-selectin is important for eosinophil rolling under these conditions, P-selectin plays only a minor role.
Type of Medium:
Online Resource
ISSN:
0022-1767
,
1550-6606
DOI:
10.4049/jimmunol.163.1.343
Language:
English
Publisher:
The American Association of Immunologists
Publication Date:
1999
detail.hit.zdb_id:
1475085-5
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