In:
Vascular, SAGE Publications, Vol. 28, No. 5 ( 2020-10), p. 619-628
Abstract:
To detect the vascular tension and nitric oxide (NO) release synchronously in mice pulmonary artery, we perform two experiments and present a novel application of confocal wire myograph coupled with the confocal laser scanning microscopy. Methods In the first experiment, viable endothelium-intact mouse pulmonary artery (outer diameter 100–300 μM) rings underwent a one-hour preincubation with a NO-specific fluorescent dye, 4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate Calbiochem (2.5 μM), and then precontracted with phenylephrine (Phen, 10 −6 M), and subsequently dilated in acetylcholine (ACh, 10 −6 M – 10 −4 M). The endothelium-dependent vasorelaxation and NO generation in pulmonary artery rings were simultaneously recorded. In the second experiment, after 30-min incubation with the former NO fluorescent dye, the qualified pulmonary artery rings were co-incubated for another 30 min with a nitric oxide synthase inhibitor, 10 −4 M Nω-nitro-L-arginine-methyl-ester (L-NAME), and then pretreated with Phen (10 −6 M) followed by ACh (10 −5 M). The Ach-induced vasodilation and NO release were recorded simultaneously. Results ACh (10 −6 M – 10 −4 M) promoted pulmonary artery relaxation and intracellular NO release in a dose-dependent manner. Additionally, L-NAME (10 −4 M) significantly attenuated the vasodilatation and the intracellular NO release. Conclusions This combined application visually confirms that the synchronous changes in Ach induced vasodilation and NO release, which provides a new method for cardiovascular research.
Type of Medium:
Online Resource
ISSN:
1708-5381
,
1708-539X
DOI:
10.1177/1708538120917555
Language:
English
Publisher:
SAGE Publications
Publication Date:
2020
detail.hit.zdb_id:
2143006-8
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