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  • 1
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    Heart Development, Coronary Vascularization and Ventricular Maturation in a Giant Danio (Devario malabaricus)
    MDPI AG ; 2018
    In:  Journal of Developmental Biology Vol. 6, No. 3 ( 2018-07-21), p. 19-
    Details
    In: Journal of Developmental Biology, MDPI AG, Vol. 6, No. 3 ( 2018-07-21), p. 19-
    Abstract: Giant danios (genus Devario), like zebrafish, are teleosts belonging to the danioninae subfamily of cyprinids. Adult giant danios are used in a variety of investigations aimed at understanding cellular and physiological processes, including heart regeneration. Despite their importance, little is known about development and growth in giant danios, or their cardiac and coronary vessels development. To address this scarcity of knowledge, we performed a systematic study of a giant danio (Devario malabaricus), focusing on its cardiac development, from the segmentation period to ten months post-fertilization. Using light and scanning electron microscopy, we documented that its cardiovascular development and maturation proceed along well defined dynamic and conserved morphogenic patterns. The overall size and cardiovascular expansion of this species was significantly impacted by environmental parameters such as rearing densities. The coronary vasculature began to emerge in the late larval stage. More importantly, we documented two possible loci of initiation of the coronary vasculature in this species, and compared the emergence of the coronaries to that of zebrafish and gourami. This is the first comprehensive study of the cardiac growth in a Devario species, and our findings serve as an important reference for further investigations of cardiac biology using this species.
    Type of Medium: Online Resource
    ISSN: 2221-3759
    URL: Article
    DOI: 10.3390/jdb6030019
    Language: English
    Publisher: MDPI AG
    Publication Date: 2018
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  • 2
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    Differential Lectin Binding Patterns Identify Distinct Heart Regions in Giant Danio ( Devario aequipinnatus ) and Zebrafish ( Danio rerio ) Hearts
    SAGE Publications ; 2016
    In:  Journal of Histochemistry & Cytochemistry Vol. 64, No. 11 ( 2016-11), p. 687-714
    Details
    In: Journal of Histochemistry & Cytochemistry, SAGE Publications, Vol. 64, No. 11 ( 2016-11), p. 687-714
    Abstract: Lectins are carbohydrate-binding proteins commonly used as biochemical and histochemical tools to study glycoconjugate (glycoproteins, glycolipids) expression patterns in cells, tissues, including mammalian hearts. However, lectins have received little attention in zebrafish ( Danio rerio) and giant danio ( Devario aequipinnatus) heart studies. Here, we sought to determine the binding patterns of six commonly used lectins—wheat germ agglutinin (WGA), Ulex europaeus agglutinin, Bandeiraea simplicifolia lectin (BS lectin), concanavalin A (Con A), Ricinus communis agglutinin I (RCA I), and Lycopersicon esculentum agglutinin (tomato lectin)—in these hearts. Con A showed broad staining in the myocardium. WGA stained cardiac myocyte borders, with binding markedly stronger in the compact heart and bulbus. BS lectin, which stained giant danio coronaries, was used to measure vascular reconstruction during regeneration. However, BS lectin reacted poorly in zebrafish. RCA I stained the compact heart of both fish. Tomato lectin stained the giant danio, and while low reactivity was seen in the zebrafish ventricle, staining was observed in their transitional cardiac myocytes. In addition, we observed unique staining patterns in the developing zebrafish heart. Lectins’ ability to reveal differential glycoconjugate expression in giant danio and zebrafish hearts suggests they can serve as simple but important tools in studies of developing, adult, and regenerating fish hearts.
    Type of Medium: Online Resource
    ISSN: 0022-1554 , 1551-5044
    URL: Article
    DOI: 10.1369/0022155416667928
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2016
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    detail.hit.zdb_id: 1421306-0
    SSG: 12
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  • 3
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    Absence of Cardiomyocyte Differentiation Following Transplantation of Adult Cardiac-Resident Sca-1 + Cells Into Infarcted Mouse Hearts
    Ovid Technologies (Wolters Kluwer Health) ; 2018
    In:  Circulation Vol. 138, No. 25 ( 2018-12-18), p. 2963-2966
    Details
    In: Circulation, Ovid Technologies (Wolters Kluwer Health), Vol. 138, No. 25 ( 2018-12-18), p. 2963-2966
    Type of Medium: Online Resource
    ISSN: 0009-7322 , 1524-4539
    URL: Article
    DOI: 10.1161/CIRCULATIONAHA.118.035391
    Language: English
    Publisher: Ovid Technologies (Wolters Kluwer Health)
    Publication Date: 2018
    detail.hit.zdb_id: 1466401-X
    detail.hit.zdb_id: 80099-5
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  • 4
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    The Giant Danio ( D. Aequipinnatus ) as A Model of Cardiac Remodeling and Regeneration
    Wiley ; 2012
    In:  The Anatomical Record Vol. 295, No. 2 ( 2012-02), p. 234-248
    Details
    In: The Anatomical Record, Wiley, Vol. 295, No. 2 ( 2012-02), p. 234-248
    Abstract: The paucity of mammalian adult cardiac myocytes (CM) proliferation following myocardial infarction (MI) and the remodeling of the necrotic tissue that ensues, result in non‐regenerative repair. In contrast, zebrafish (ZF) can regenerate after an apical resection or cryoinjury of the heart. There is considerable interest in models where regeneration proceeds in the presence of necrotic tissue. We have developed and characterized a cautery injury model in the giant danio (GD), a species closely related to ZF, where necrotic tissue remains part of the ventricle, yet regeneration occurs. By light and transmission electron microscopy (TEM), we have documented four temporally overlapping processes: (1) a robust inflammatory response analogous to that observed in MI, (2) concomitant proliferation of epicardial cells leading to wound closure, (3) resorption of necrotic tissue and its replacement by granulation tissue, and (4) regeneration of the myocardial tissue driven by 5‐EDU and [ 3 H]thymidine incorporating CMs. In conclusion, our data suggest that the GD possesses robust repair mechanisms in the ventricle and can serve as an important model of cardiac inflammation, remodeling and regeneration. Anat Rec, 2012. © 2011 Wiley Periodicals, Inc.
    Type of Medium: Online Resource
    ISSN: 1932-8486 , 1932-8494
    URL: Issue
    URL: Article
    DOI: 10.1002/ar.v295.2
    DOI: 10.1002/ar.21492
    Language: English
    Publisher: Wiley
    Publication Date: 2012
    detail.hit.zdb_id: 2269667-2
    detail.hit.zdb_id: 2109216-3
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  • 5
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    The CUL7 E3 Ubiquitin Ligase Targets Insulin Receptor Substrate 1 for Ubiquitin-Dependent Degradation
    Elsevier BV ; 2008
    In:  Molecular Cell Vol. 30, No. 4 ( 2008-05), p. 403-414
    Details
    In: Molecular Cell, Elsevier BV, Vol. 30, No. 4 ( 2008-05), p. 403-414
    Type of Medium: Online Resource
    ISSN: 1097-2765
    URL: Article
    DOI: 10.1016/j.molcel.2008.03.009
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2008
    detail.hit.zdb_id: 1415236-8
    SSG: 12
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  • 6
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    Structural and Functional Characterization of the Adult Giant Danio Heart
    Wiley ; 2019
    In:  The FASEB Journal Vol. 33, No. S1 ( 2019-04)
    Details
    In: The FASEB Journal, Wiley, Vol. 33, No. S1 ( 2019-04)
    Abstract: Cardiovascular diseases remain that number one cause of death in the northern hemisphere and are increasing globally in developing economies. In spite of evolutionary separation between fishes and mammalian, the use of non‐mammalian model species has significantly increased our understanding of cardiac diseases. We have recently described the growth and cardiovascular development of the Devario malabaricus , a giant danio (GD) species closely related to the zebrafish. We hypothesize that the adult GD heart can serve as a robust non‐mammalian model for in vivo studies of cardiac biology. Using immunostaining, transmission electron microscopy, scanning block‐face electron microscopy, and Doppler, we have characterized the anatomical and functional characteristics of the adult GD heart. First, we found that the adult giant danio possesses a thick and highly vascularized compact heart. Second, the GD heart possesses a junctional region populated with a fibroblasts network similar to that observed in zebrafish. Third, multiple large coronary vessels investing the compact myocardium are connected to and continuous with atrioventricular (AV) canal lumen. In addition, the AV canal and bulbus are highly innervated, suggesting complex regulation in heart function. Moreover, we demonstrate that their cardiac function can be measured using Doppler flow velocity. These studies along with previous findings support the GD as a robust model for the study of cardiac biology. Support or Funding Information 1R15HD084262‐01, Buehler Family Foundation This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .
    Type of Medium: Online Resource
    ISSN: 0892-6638 , 1530-6860
    URL: Issue
    URL: Article
    DOI: 10.1096/fsb2.v33.S1
    DOI: 10.1096/fasebj.2019.33.1_supplement.771.1
    Language: English
    Publisher: Wiley
    Publication Date: 2019
    detail.hit.zdb_id: 1468876-1
    detail.hit.zdb_id: 639186-2
    SSG: 12
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  • 7
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    Inflammation, Collagen Deposition, and Healing in a Goldfish (Carassius auratus) Model of Heart Injury
    Wiley ; 2009
    In:  The FASEB Journal Vol. 23, No. S1 ( 2009-04)
    Details
    In: The FASEB Journal, Wiley, Vol. 23, No. S1 ( 2009-04)
    Abstract: Heart disease remains the number one cause of death in the United States. Mammalian and non‐mammalian models are used to study heart healing and regeneration following injury. In the teleost zebrafish , the removal of ventricular tissue by resection is fully replaced by thirty days with little to no scarring. We hypothesized that the goldfish ( Carassius auratus) is able to regenerate its heart in a manner analogous to the zebrafish. We have performed cautery injury of 25% of the ventricle of goldfish that resulted in significant destruction of the ventricular wall and ventricular myocytes. We found an intense inflammatory response in the area of injury, followed the recruitment or proliferation of cells 3 days post injury. We also found a marked increased in collagen deposition at 3 days and 7 days that is resolved by 21 days. This observation is in contrast to the lack of collagen deposition reported in other models. Our data suggests that following cautery injury the goldfish ventricular wall is rebuilt by one week and a quasi complete healing is observed by 21 days. This is the first study showing healing following cautery injury in a fish heart. Funding DePauw University
    Type of Medium: Online Resource
    ISSN: 0892-6638 , 1530-6860
    URL: Issue
    URL: Article
    DOI: 10.1096/fsb2.v23.S1
    DOI: 10.1096/fasebj.23.1_supplement.235.8
    Language: English
    Publisher: Wiley
    Publication Date: 2009
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    detail.hit.zdb_id: 639186-2
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  • 8
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    Cautery Injury Response in the Zebrafish Heart
    Wiley ; 2017
    In:  The FASEB Journal Vol. 31, No. S1 ( 2017-04)
    Details
    In: The FASEB Journal, Wiley, Vol. 31, No. S1 ( 2017-04)
    Abstract: Using cauterization of the ventricular myocardium, previous studies in our lab have shown evidence of matrix remodeling as well as robust cardiac regeneration in the giant danio, a species closely related to the zebrafish. Various approaches to ventricular injury, including resection, cryoinjury, and genetic ablation have been used in zebrafish, resulting in differential responses in cardiac remodeling, the initiation and the rate of progression of cardiac regeneration. To date, responses to cardiac cautery injury have not been studied in the zebrafish heart. Hence, we aimed to characterize the ventricular myocardium response to this particular approach to heart injury. Using light and electron microscopy, in conjunction with myeloperoxidase staining, Edu incorporation in cardiac myocytes, Masson's Trichome and picrosirius red/fast green staining, we studied the repair of cauterized zebrafish hearts from 3 to 90 days post‐surgery. We found that cauterization results in regional tissue necrosis that was followed by marked infiltration of inflammatory cells, subsequent accumulation and resorption of connective tissue, and a gradual regenerative response. In addition, we used wheat germ agglutinin staining to document the progressive reconstruction of the damaged compact/cortical myocardium. Our study shows that ventricular cauterization is a simple and reproducible alternative approach to create myocardial necrosis, and to elicit matrix remodeling and cardiac regenerative responses in the zebrafish heart. Support or Funding Information P.J.L. is supported by NIH 1 R15 HD084262‐01, Donald E. Town Faculty Fellowship, and Faculty Development Funds at DePauw University
    Type of Medium: Online Resource
    ISSN: 0892-6638 , 1530-6860
    URL: Issue
    URL: Article
    DOI: 10.1096/fsb2.v31.S1
    DOI: 10.1096/fasebj.31.1_supplement.586.2
    Language: English
    Publisher: Wiley
    Publication Date: 2017
    detail.hit.zdb_id: 1468876-1
    detail.hit.zdb_id: 639186-2
    SSG: 12
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  • 9
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    Cauterization as a Simple Method for Regeneration Studies in the Zebrafish Heart
    MDPI AG ; 2020
    In:  Journal of Cardiovascular Development and Disease Vol. 7, No. 4 ( 2020-10-03), p. 41-
    Details
    In: Journal of Cardiovascular Development and Disease, MDPI AG, Vol. 7, No. 4 ( 2020-10-03), p. 41-
    Abstract: In the last two decades, the zebrafish has emerged as an important model species for heart regeneration studies. Various approaches to model loss of cardiac myocytes and myocardial infarction in the zebrafish have been devised, and have included resection, genetic ablation, and cryoinjury. However, to date, the response of the zebrafish ventricle to cautery injury has not been reported. Here, we describe a simple and reproducible method using cautery injury via a modified nichrome inoculating needle as a probe to model myocardial infarction in the zebrafish ventricle. Using light and electron microscopy, we show that cardiac cautery injury is attended by significant inflammatory cell infiltration, accumulation of collagen in the injured area, and the reconstitution of the ventricular myocardium. Additionally, we document the ablation of cardiac nerve fibers, and report that the re-innervation of the injured zebrafish ventricle is protracted, compared to other repair processes that accompany the regeneration of the cauterized ventricle. Taken together, our study demonstrates that cautery injury is a simple and effective means for generating necrotic tissue and eliciting a remodeling and regenerative response in the zebrafish heart. This approach may serve as an important tool in the methods toolbox for regeneration studies in the zebrafish.
    Type of Medium: Online Resource
    ISSN: 2308-3425
    URL: Article
    DOI: 10.3390/jcdd7040041
    Language: English
    Publisher: MDPI AG
    Publication Date: 2020
    detail.hit.zdb_id: 2777082-5
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  • 10
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    Ventricular Growth and Coronary Vessel Development in the Giant Danio ( Devario Aequipinnatus ) Heart
    Wiley ; 2017
    In:  The FASEB Journal Vol. 31, No. S1 ( 2017-04)
    Details
    In: The FASEB Journal, Wiley, Vol. 31, No. S1 ( 2017-04)
    Abstract: The adult giant danio (GD) heart displays robust regenerative response to cardiac injury, similar to that observed in the closely related species, the zebrafish (ZF). However, unlike the ZF, little is known about cardiovascular growth and ventricular maturation of the GD. We studied over 300 GD larva, juveniles and adults using light and electron microscopy. We have documented the dynamic changes in specific anatomical and physiological growth parameters from 7 days to 32 weeks post‐fertilization (wpf) adults. While we found that the GD displayed marked variations in sizes/standard lengths (SL) and ventricular sizes/ventricular lengths (VL) in their respective age groups, GD VL were closely correlated with their SL. GD SL increased by an average of 1.2 mm from 1 to10 wpf, and VL increasing by 50 um/week from 5 to 10 wpf. We also found that GD raised at low densities (1–2/liter) experienced greater cardiac growth, and lesser SL and VL variance than fish grown at higher densities (5–10/liter). Like the ZF, the coronary vasculature emerged in the late larval stage, and both the initiation and expansion of the vasculature was dependent upon SL. The switch from an a vascular to vascularized myocardium was concomitant with the appearance and increase in thickness of the compact heart, and the formation of a junctional region occupied by a spatially organized fibroblasts network. This is the first comprehensive study of the cardiac growth and maturation in the GD heart from larval stage to adulthood. Our new findings may serve as an important baseline for comparative cardiac biology studies in fish species including the ZF. Support or Funding Information P.J.L. is supported by NIH 1 R15 HD084262‐01, the Donald E. Town Faculty Fellowship, and Faculty Development Funds at DePauw University
    Type of Medium: Online Resource
    ISSN: 0892-6638 , 1530-6860
    URL: Issue
    URL: Article
    DOI: 10.1096/fsb2.v31.S1
    DOI: 10.1096/fasebj.31.1_supplement.586.13
    Language: English
    Publisher: Wiley
    Publication Date: 2017
    detail.hit.zdb_id: 1468876-1
    detail.hit.zdb_id: 639186-2
    SSG: 12
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