In:
Intervirology, S. Karger AG, Vol. 54, No. 5 ( 2011), p. 282-289
Abstract:
〈 i 〉 Objective: 〈 /i 〉 Partial genome sequences were determined and subjected to comparative analyses from two fish herpesviruses (HVs). Acipenserid (Aci) HV-2, originating from the white sturgeon 〈 i 〉 (Acipenser transmontanus) 〈 /i 〉 , and ictalurid (Ic) HV-2, isolated from the black bullhead 〈 i 〉 (Ameiurus melas), 〈 /i 〉 are recently approved species of the genus 〈 i 〉 Ictalurivirus 〈 /i 〉 of the family 〈 i 〉 Alloherpesviridae 〈 /i 〉 . 〈 i 〉 Methods: 〈 /i 〉 An almost 8,000-base-pair fragment, spanning between the genes of the DNA polymerase and the ATPase subunit of the terminase, was sequenced from each virus. 〈 i 〉 Results: 〈 /i 〉 The size, position and orientation of 2 partial and 3 full open reading frames, contained in the studied genome fragment, proved to be similar to their counterparts in IcHV-1, the type species of the genus 〈 i 〉 Ictalurivirus 〈 /i 〉 . Thus, a well-conserved genus-specific gene block was identified. In the members of two other genera 〈 i 〉 (Cyprinivirus 〈 /i 〉 and 〈 i 〉 Batrachovirus) 〈 /i 〉 of the family 〈 i 〉 Alloherpesviridae 〈 /i 〉 , no such gene block could be found; the location and orientation of the homologous genes showed significant divergence. 〈 i 〉 Conclusion: 〈 /i 〉 The results of phylogenetic calculations were in good agreement with the genome arrangements inasmuch as AciHV-2, IcHV-1 and -2 are monophyletic and separated from the lineages of the other two genera. The new sequence enabled the inclusion of a hitherto unassigned HV, that of the Australian pilchard, into a phylogenetic calculation.
Type of Medium:
Online Resource
ISSN:
0300-5526
,
1423-0100
Language:
English
Publisher:
S. Karger AG
Publication Date:
2011
detail.hit.zdb_id:
1482863-7
SSG:
12
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