In:
The Journal of Immunology, The American Association of Immunologists, Vol. 109, No. 6 ( 1972-12-01), p. 1386-1394
Abstract:
The Fab-anti-Fab assay for human IgG quantitation is used to measure in vitro IgG production by lymphocytes from five normal human lymphoid tissues: bone marrow, spleen, blood, lymph node and thymus. Synthesis of IgG was found to proceed at a constant rate over the 10-day culture period; production could be prevented by hypothermia or mitomycin-C. When corrected for total lymphoid cells, the bone marrow was noted to produce greater than 95% of the IgG synthesized by the organs which were evaluated. Secondary antigenic stimulation with smallpox vaccine resulted in accelerated IgG synthesis by blood and splenic lymphocytes; conversely, no response was noted in cultures containing marrow or thymic cells. It is concluded that the bone marrow must be considered as a major site of IgG production under normal circumstances; however, the inability of the bone marrow to respond to antigenic stimulation suggests that the appropriate quantity or quality of cells is lacking in this organ for optimal antigenic recognition and/or processing.
Type of Medium:
Online Resource
ISSN:
0022-1767
,
1550-6606
DOI:
10.4049/jimmunol.109.6.1386
Language:
English
Publisher:
The American Association of Immunologists
Publication Date:
1972
detail.hit.zdb_id:
1475085-5
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