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  • 1
    Online Resource
    Online Resource
    Pseudomonas lijiangensis sp. nov., a novel phytopathogenic bacterium isolated from black spots of tobacco
    Microbiology Society ; 2022
    In:  International Journal of Systematic and Evolutionary Microbiology Vol. 72, No. 10 ( 2022-10-25)
    Details
    In: International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 72, No. 10 ( 2022-10-25)
    Abstract: Three Gram-stain-negative, motile, with amphilophotrichous flagella, and rod-shaped bacteria (LJ1, LJ2 T and LJ3) were isolated from lower leaves with black spots on flue-cured tobacco in Yunnan, PR China. The results of phylogenetic analysis based on 16S rRNA gene sequences indicate that all the strains from tobacco were closely related to the type strains of the Pseudomonas syringae group within the P. fluorescens lineage and LJ2 T has the highest sequence identities with P. cichorii DSM 50259 T (99.92 %), P. capsici Pc19-1 T (99.67 %) and P. ovata F51 T (98.94 %) . The 16S rRNA gene sequence identities between LJ2 T and other members of the genus Pseudomonas were below 98.50%. The average nucleotide identity by blast (ANIb) values between LJ2 T and P. cichorii DSM 50259 T , P. capsici Pc19-1 T and P. ovata F51 T were less than 95 %, and the in silico DNA–DNA hybridization ( is DDH) values (yielded by formula 2) were less than 70 %. The major fatty acids were C 16  :  1 ω7 c and/or C 16  :  1 ω6 c (summed feature 3), C 16  :  0 and C 18  :  1 ω7 c and/or C 18  :  1 ω6 c (summed feature 8). The polar lipids profile of LJ2 T consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, two unidentified phospholipids and one unidentified glycolipid. The predominant respiratory quinone was Q-9. The DNA G+C content of LJ2 T was 58.4 mol%. On the basis of these data, we concluded that LJ2 T represents a novel species of the genus Pseudomonas , for which the name Pseudomonas lijiangensis sp. nov. is proposed. The type strain of Pseudomonas lijiangensis sp. nov. is LJ2 T (=CCTCC AB 2021465 T =GDMCC 1.2884 T =JCM 35177 T ).
    Type of Medium: Online Resource
    ISSN: 1466-5026 , 1466-5034
    URL: Article
    DOI: 10.1099/ijsem.0.005591
    Language: English
    Publisher: Microbiology Society
    Publication Date: 2022
    detail.hit.zdb_id: 215062-1
    detail.hit.zdb_id: 2056611-6
    SSG: 12
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  • 2
    Online Resource
    Online Resource
    Ralstonia wenshanensis sp. nov., a novel bacterium isolated from a tobacco field in Yunnan, China
    Microbiology Society ; 2022
    In:  International Journal of Systematic and Evolutionary Microbiology Vol. 72, No. 12 ( 2022-12-13)
    Details
    In: International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 72, No. 12 ( 2022-12-13)
    Abstract: A Gram-negative, aerobic, motile with paired polar flagella and rod-shaped bacterium strain (56D2 T ) was isolated from tobacco planting soil in Yunnan, PR China. Major fatty acids were C 16  :  1 ω 7 c (summed feature 3), C 16  :  0 and C 18  :  1   ω 7 c (summed feature 8). The polar lipid profile of strain 56D2 T consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminophospholipid and one unidentified glycolipid. Moreover, strain 56D2 T contained ubiquinone Q-8 as the sole respiratory quinone. 16S rRNA gene sequence analysis showed that strain 56D2 T was closely related to members of the genus Ralstonia and the two type strains with the highest sequence identities were R. mannitolilytica LMG 6866 T (98.36 %) and R. pickettii K-288 T (98.22 %). The 16S rRNA gene sequence identities between strain 56D2 T and other members of the genus Ralstonia were below 98.00 %. Genome sequencing revealed a genome size of 5.87 Mb and a G+C content of 63.7 mol%. The average nucleotide identity values between strain 56D2 T and R. pickettii K-288 T , R. mannitolilytica LMG 6866 T and R. insidiosa CCUG 46789 T were less than 95 %, and the in silico DNA–DNA hybridization values (yielded by formula 2) were less than 70 %. Based on these data, we conclude that strain 56D2 T represents a novel species of the genus Ralstonia , for which the name Ralstonia wenshanensis sp. nov. is proposed. The type strain of Ralstonia wenshanensis sp. nov. is 56D2 T (=CCTCC AB 2021466 T =GDMCC 1.2886 T =JCM 35178 T ).
    Type of Medium: Online Resource
    ISSN: 1466-5026 , 1466-5034
    URL: Article
    DOI: 10.1099/ijsem.0.005622
    Language: English
    Publisher: Microbiology Society
    Publication Date: 2022
    detail.hit.zdb_id: 215062-1
    detail.hit.zdb_id: 2056611-6
    SSG: 12
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  • 3
    Online Resource
    Online Resource
    Purification and characterization of White Spot Syndrome Virus (WSSV) produced in an alternate host: crayfish, Cambarus clarkii
    Elsevier BV ; 2001
    In:  Virus Research Vol. 76, No. 2 ( 2001-8), p. 115-125
    Details
    In: Virus Research, Elsevier BV, Vol. 76, No. 2 ( 2001-8), p. 115-125
    Type of Medium: Online Resource
    ISSN: 0168-1702
    URL: Article
    DOI: 10.1016/S0168-1702(01)00247-7
    RVK:
    XA 10000
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2001
    detail.hit.zdb_id: 1500820-4
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  • 4
    Online Resource
    Online Resource
    Lateral approach is a more aesthetical option for radical resection of BSCC: assessment of its surgical, oncological, functional, and aesthetic outcomes
    Springer Science and Business Media LLC ; 2022
    In:  BMC Oral Health Vol. 22, No. 1 ( 2022-11-03)
    Details
    In: BMC Oral Health, Springer Science and Business Media LLC, Vol. 22, No. 1 ( 2022-11-03)
    Abstract: The purpose of this study was to introduce a modified lateral approach for combined radical resection of buccal squamous cell carcinoma (BSCC) and evaluate its surgical, oncological, functional, and aesthetic outcomes in comparison with the conventional lower-lip splitting approach. Methods This single-center study retrospectively reviewed 80 patients with BSCC, of which 37 underwent the lateral approach and 43 underwent the conventional approach. Surgical, functional, oncological, and aesthetic evaluations, as well as follow-ups, were recorded and compared. Results Compared to the conventional approach group, the lateral approach group had a longer surgical time ( P  = 0.000), but there was no significant difference in other surgical and oncological parameters. Moreover, the scar in the head and neck had a significantly discreet appearance in the lateral approach group, whose satisfaction was better than those in the conventional approach group ( P  = 0.000). Other oral function parameters, postoperative mouth-opening, and 3-year survival rate were not significantly different between the two groups. Conclusion The lateral approach could provide superior aesthetic results while maintaining equal surgical, functional, and oncological outcomes compared to the conventional approach for radical resection of BSCC.
    Type of Medium: Online Resource
    ISSN: 1472-6831
    URL: Article
    DOI: 10.1186/s12903-022-02519-1
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2022
    detail.hit.zdb_id: 2091511-1
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  • 5
    Online Resource
    Online Resource
    Keratin 17 identified by proteomic analysis may be involved in tumor angiogenesis
    Korean Society for Biochemistry and Molecular Biology - BMB Reports ; 2009
    In:  BMB Reports Vol. 42, No. 6 ( 2009-06-30), p. 344-349
    Details
    In: BMB Reports, Korean Society for Biochemistry and Molecular Biology - BMB Reports, Vol. 42, No. 6 ( 2009-06-30), p. 344-349
    Type of Medium: Online Resource
    ISSN: 1976-6696
    URL: Article
    DOI: 10.5483/BMBRep.2009.42.6.344
    Language: English
    Publisher: Korean Society for Biochemistry and Molecular Biology - BMB Reports
    Publication Date: 2009
    detail.hit.zdb_id: 2410393-7
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  • 6
    Online Resource
    Online Resource
    Scanning tunneling microscopy study of h-BN thin films grown on Cu foils
    Acta Physica Sinica, Chinese Physical Society and Institute of Physics, Chinese Academy of Sciences ; 2016
    In:  Acta Physica Sinica Vol. 65, No. 11 ( 2016), p. 116801-
    Details
    In: Acta Physica Sinica, Acta Physica Sinica, Chinese Physical Society and Institute of Physics, Chinese Academy of Sciences, Vol. 65, No. 11 ( 2016), p. 116801-
    Abstract: Analogous to graphite, hexagonal boron nitride (h-BN) has a layered structure composed of boron and nitrogen atoms that are alternatively bond to each other in a honeycomb array. As the layers are held together by weak van der Waals forces, h-BN thin films can be grown on surfaces of various metal crystals in a layer-by-layer manner, which is again similar to graphene sheets and thus attracts a lot of research interests. In this work, scanning tunneling microscope and spectroscope (STM and STS) were applied to the study of an h-BN thin film with a thickness of about 10 nm grown on Cu foil by means of chemical vapor deposition. X-ray diffraction from the Cu foil shows only one strong peak of Cu(200) in the angle range of 40-60, indicating that the Cu foil is mainly Cu(100). After sufficient annealing in an UHV chamber, the h-BN film sample is transferred to a cooling stage (77 K) for STM/STS measurement. Its high quality is confirmed by a large-scale STM scan that shows an atomically flat topography. A series of dI/dV data taken within varied energy windows all exhibit similar U shapes but with different bottom widths that monotonously decrease with the sweeping energy window. The dI/dV curve taken in the energy window of [-1 V, +1 V] even shows no energy gap in spite that h-BN film is insulating with a quite large energy gap of around 6 eV, as observed in a large-energy-window dI/dV curve (from -5 V to +5 V). These results indicate that the STM images reflect the spatial distribution of tunneling barriers between Cu(100) substrate and STM tip, rather than the local density of states of the h-BN surface. At high sample biases (from 4 V to 1 V), STM images exhibit an electronic modulation pattern with short range order. The modulation pattern displays a substructure in low-bias STM images (less than 100 mV), which finally turns to the (11) lattice of h-BN surface when the sample bias is extremely lowered to 3 mV. It is found that the electronic modulation pattern cannot be fully reproduced by superimposing hexagonal BN lattice on tetragonal Cu(100) lattice, no matter what their relative in-plane crystal orientation is. This implies that the electronic modulation pattern in the STM images is not a Mori pattern due to lattice mismatch. We speculate that it may originate from spatial distribution of tunneling barrier induced by adsorption of H, B and/or N atoms on the Cu(100) surface in the CVD growth process.
    Type of Medium: Online Resource
    ISSN: 1000-3290 , 1000-3290
    URL: Journal
    URL: Article
    DOI: 10.7498/aps
    DOI: 10.7498/aps.65.116801
    Language: Unknown
    Publisher: Acta Physica Sinica, Chinese Physical Society and Institute of Physics, Chinese Academy of Sciences
    Publication Date: 2016
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  • 7
    Online Resource
    Online Resource
    Scanning tunneling microscopy research of Bi(110) thin films grown on NbSe2
    Acta Physica Sinica, Chinese Physical Society and Institute of Physics, Chinese Academy of Sciences ; 2018
    In:  Acta Physica Sinica Vol. 67, No. 17 ( 2018), p. 170701-
    Details
    In: Acta Physica Sinica, Acta Physica Sinica, Chinese Physical Society and Institute of Physics, Chinese Academy of Sciences, Vol. 67, No. 17 ( 2018), p. 170701-
    Abstract: Due to the novel physical properties induced by the strong spin orbit coupling and band inversions in the energy band structure, two-dimensional topological insulator has become a hot research point in the field of condensed matter physics and material science in recent years. Particularly, two-dimensional topological insulator may host exotic Majorana fermionic excitations in its edge state if superconductivity is introduced. Bi thin film with (111) orientation proves to be a two-dimensional topological insulator both in theory and in experiment. However, the topological nature of Bi thin film with (110) orientation has not yet been confirmed. In this study, high quality Bi(110) thin films are successfully prepared on superconductor NbSe2 surfaces, by the molecular beam epitaxial technology at ambient temperature and a low deposition rate (~24℃,~3 min/bilayer). The morphologies and electronic properties of the samples are studied by using scanning tunneling microscopy and spectroscopy. The experimental results reveal that the growth mode changes from bilayer (BL) in BL mode to monolayer (ML) in ML mode. Such transition takes place at a critical height of about 4 BLs. The mechanism of the growth mode transition is believed to be induced by the drastic variation of the surface energies of the thin films with different thickness values. Due to the large coverage of Bi(110) film on the NbSe2 substrate, it is almost impossible to find the exposed areas of NbSe2 substrate surface in practice. Especially on the sample with a large number of layers of Bi thin film, it is hard to directly determine the number of layers for each film. Hence, the critical thickness could be only estimated by controlling the deposition time and growth rate combining with the measurements of stage height of the film. The nearly identical local density of states wherever measured in the interior of a terrace or at the step edges can be discerned from the dI/dV spectra, which is thus hard to corroborate with non-trivial topology in either BL or ML thick Bi(110) film. The superconductivity induced by proximity effect from the superconducting substrate NbSe2 is also observed on the thin films. Through Bardeen-Cooper-Schrieffer type data fitting, the superconducting gap on the Bi thin film is estimated at about 0.5 meV. In addition, the quantum well state, which is often observed in thin films, is also revealed from the Bi(110) thin films, whose characteristic is equal energy spacing between peaks in dI/dV spectra. Noticeably, the spectral shapes of BL and ML are similar, and the local density of states from adjacent film layers displays an approximate πup phase shift.
    Type of Medium: Online Resource
    ISSN: 1000-3290 , 1000-3290
    URL: Journal
    URL: Article
    DOI: 10.7498/aps
    DOI: 10.7498/aps.67.20180977
    Language: Unknown
    Publisher: Acta Physica Sinica, Chinese Physical Society and Institute of Physics, Chinese Academy of Sciences
    Publication Date: 2018
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  • 8
    Online Resource
    Online Resource
    Data_Sheet_1.docx
    Frontiers Media SA ; 2023
    In:  Frontiers in Microbiology Vol. 14 ( 2023-5-5)
    Details
    In: Frontiers in Microbiology, Frontiers Media SA, Vol. 14 ( 2023-5-5)
    Abstract: Eight Gram-negative, aerobic, motile with paired polar flagella and rod-shaped bacteria were isolated from six tobacco fields in Yunnan, PR China. 16S rRNA gene sequence analysis revealed that all the strains belonged to the genus Ralstonia . Among them, strain 22TCCZM03-6 had an identical 16S rRNA sequence to that of R. wenshanensis 56D2 T , and the other strains were closely related to R. pickettii DSM 6297 T (98.34–99.86%), R. wenshanensis 56D2 T (98.70–99.64%), and R. insidiosa CCUG 46789 T (97.34–98.56%). Genome sequencing yielded sizes ranging from 5.17 to 5.72 Mb, with overall G + C contents of 63.3–64.1%. Pairwise genome comparisons showed that strain 22TCCZM03-6 shared average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values above the species cut-off with R. wenshanensis 56D2 T , suggesting that strain 22TCCZM03-6 is a special strain of the R. wenshanensis . Five strains, including 21MJYT02-10 T , 21LDWP02-16, 22TCJT01-1, 22TCCZM01-4, and 22TCJT01-2, had ANI values & gt;95% and dDDH values & gt;70% when compared with each other. These five strains had ANI values of 73.32–94.17% and dDDH of 22.0–55.20% with the type strains of the genus Ralstonia individually, supporting these five strains as a novel species in the genus Ralstonia . In addition, strains 21YRMH01-3 T and 21MJYT02-11 T represent two independent species. They both had ANI and dDDH values below the thresholds for species delineation when compared with the type species of the genus Ralstonia . In strains 21YRMH01-3 T and 21MJYT02-10 T , the main fatty acids were summed features 3, 8, and C 16:0 ; however, strain 21MJYT02-11 T contained C 16:0 , cyclo-C 17:0 , and summed features 3 as major fatty acids. The main polar lipids, including diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine, were identified from strains 21YRMH01-3 T , 21MJYT02-10 T , and 21MJYT02-11 T . The ubiquinones Q-7 and Q-8 were also detected in these strains, with Q-8 being the predominant quinone. Based on the above data, we propose that the eight strains represent one known species and three novel species in the genus Ralstonia , for which the names Ralstonia chuxiongensis sp. nov., Ralstonia mojiangensis sp. nov., and Ralstonia soli sp. nov. are proposed. The type strains are 21YRMH01-3 T (=GDMCC 1.3534 T  = JCM 35818 T ), 21MJYT02-10 T (=GDMCC 1.3531 T  = JCM 35816 T ), and 21MJYT02-11 T (=GDMCC 1.3532 T  = JCM 35817 T ), respectively.
    Type of Medium: Online Resource
    ISSN: 1664-302X
    URL: Article
    URL: Article
    DOI: 10.3389/fmicb.2023.1179087
    DOI: 10.3389/fmicb.2023.1179087.s001
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2023
    detail.hit.zdb_id: 2587354-4
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  • 9
    Online Resource
    Online Resource
    Carrier-envelope stabilized few-cycle infrared laser system
    Acta Physica Sinica, Chinese Physical Society and Institute of Physics, Chinese Academy of Sciences ; 2011
    In:  Acta Physica Sinica Vol. 60, No. 5 ( 2011), p. 054206-
    Details
    In: Acta Physica Sinica, Acta Physica Sinica, Chinese Physical Society and Institute of Physics, Chinese Academy of Sciences, Vol. 60, No. 5 ( 2011), p. 054206-
    Abstract: According to the feature of the passive carrier-envelope phase (CEP) stabilized idler of the optical parametric amplified process, a CEP stabilized three-stage collinear optical parametric amplifier is built. Millijoule ultrashort infrared output (1.4 mJ/40 fs/1 kHz at 1.8 μm) with CEP fluctuation ~516mrad is obtained from the system. After passing through a hollow-core fiber and block material, the pulses are finally compressed to less than two cycles (〈12 fs), and the single pulse energy is 0.54mJ. The system provides an excellent laser source for the experiments of individual attosecond pulse generation and other high harmonic generation (HHG).
    Type of Medium: Online Resource
    ISSN: 1000-3290 , 1000-3290
    URL: Journal
    URL: Article
    DOI: 10.7498/aps
    DOI: 10.7498/aps.60.054206
    Language: Unknown
    Publisher: Acta Physica Sinica, Chinese Physical Society and Institute of Physics, Chinese Academy of Sciences
    Publication Date: 2011
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  • 10
    Online Resource
    Online Resource
    Human acyl-CoA:cholesterol acyltransferase 2 gene expression in intestinal Caco-2 cells and in hepatocellular carcinoma
    Portland Press Ltd. ; 2006
    In:  Biochemical Journal Vol. 394, No. 3 ( 2006-03-15), p. 617-626
    Details
    In: Biochemical Journal, Portland Press Ltd., Vol. 394, No. 3 ( 2006-03-15), p. 617-626
    Abstract: Humans express two ACAT (acyl-CoA:cholesterol acyltransferase) genes, ACAT1 and ACAT2. ACAT1 is ubiquitously expressed, whereas ACAT2 is primarily expressed in intestinal mucosa and plays an important role in intestinal cholesterol absorption. To investigate the molecular mechanism(s) responsible for the tissue-specific expression of ACAT2, we identified five cis-elements within the human ACAT2 promoter, four for the intestinal-specific transcription factor CDX2 (caudal type homeobox transcription factor 2), and one for the transcription factor HNF1α (hepatocyte nuclear factor 1α). Results of luciferase reporter and electrophoretic mobility shift assays show that CDX2 and HNF1α exert a synergistic effect, enhancing the ACAT2 promoter activity through binding to these cis-elements. In undifferentiated Caco-2 cells, the ACAT2 expression is increased when exogenous CDX2 and/or HNF1α are expressed by co-transfection. In differentiated Caco-2 cells, the ACAT2 expression significantly decreases when the endogenous CDX2 or HNF1α expression is suppressed by using RNAi (RNA interference) technology. The expression levels of CDX2, HNF1α, and ACAT2 are all greatly increased when the Caco-2 cells differentiate to become intestinal-like cells. These results provide a molecular mechanism for the tissue-specific expression of ACAT2 in intestine. In normal adult human liver, CDX2 expression is not detectable and the ACAT2 expression is very low. In the hepatoma cell line HepG2 the CDX2 expression is elevated, accounting for its elevated ACAT2 expression. A high percentage (seven of fourteen) of liver samples from patients affected with hepatocellular carcinoma exhibited elevated ACAT2 expression. Thus, the elevated ACAT2 expression may serve as a new biomarker for certain form(s) of hepatocellular carcinoma.
    Type of Medium: Online Resource
    ISSN: 0264-6021 , 1470-8728
    URL: Article
    DOI: 10.1042/BJ20051417
    RVK:
    WA 15000
    Language: English
    Publisher: Portland Press Ltd.
    Publication Date: 2006
    detail.hit.zdb_id: 1473095-9
    SSG: 12
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