In:
Bioscience, Biotechnology, and Biochemistry, Informa UK Limited, Vol. 80, No. 11 ( 2016-11-01), p. 2198-2207
Kurzfassung:
In rice, several allergens have been identified such as the non-specific lipid transfer protein-1, the α-amylase/trypsin-inhibitors, the α-globulin, the 33 kDa glyoxalase I (Gly I), the 52–63 kDa globulin, and the granule-bound starch synthetase. The goal of the present study was to define optimal rice extraction and detection methods that would allow a sensitive and reproducible measure of several classes of known rice allergens. In a three-laboratory ring-trial experiment, several protein extraction methods were first compared and analyzed by 1D multiplexed SDS-PAGE. In a second phase, an inter-laboratory validation of 2D-DIGE analysis was conducted in five independent laboratories, focusing on three rice allergens (52 kDa globulin, 33 kDa glyoxalase I, and 14–16 kDa α-amylase/trypsin inhibitor family members). The results of the present study indicate that a combination of 1D multiplexed SDS-PAGE and 2D-DIGE methods would be recommended to quantify the various rice allergens.
Materialart:
Online-Ressource
ISSN:
0916-8451
,
1347-6947
DOI:
10.1080/09168451.2016.1206810
Sprache:
Englisch
Verlag:
Informa UK Limited
Publikationsdatum:
2016
ZDB Id:
2110940-0
SSG:
12
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