In:
Transfusion, Wiley, Vol. 63, No. 2 ( 2023-02), p. 384-392
Abstract:
There is no standardized storage temperature of whole blood for acute normovolemic hemodilution (ANH). Study Design and Methods We conducted a prospective observational study to examine the difference in platelet function between short‐term whole blood storage at 4 and 22°C. Venous blood (40 ml) was collected from seven healthy subjects who gave prior written consent. The samples were divided into three groups: before storage (group Pre), cold (4°C) storage (group C), and room temperature (22°C) storage (group R). Groups C and R were tested after 6 h of blood storage. Platelet aggregability, platelet factor 4 (PF4), β ‐thromboglobulin ( β ‐TG), P‐selectin expression, pH, PO 2 , PCO 2 , glucose, lactate, blood count, and thromboelastography (TEG) parameters were measured. The percentage change in each parameter in groups C and R was calculated using the value in group Pre as a reference. These data were then compared between groups C and R using a Wilcoxon matched pairs test. p 〈 0.05 was considered to be statistically significant. Results Compared with group R, group C showed significantly higher platelet aggregability with adenosine diphosphate (ADP) 2, 4, and 6 μM (all p = 0.016) and collagen 1 μg/ml ( p = 0.047) stimulation, and significantly lower PF4 and β ‐TG elevation (both p = 0.031), glucose consumption ( p = 0.031), and lactate production ( p = 0.016). The ADP channel in TEG showed a significant increase in platelet aggregation rate in group C compared to group R. Discussion Cold storage of whole blood in ANH may provide improved storage conditions for platelets and contribute to improved hemostasis compared to room temperature storage.
Type of Medium:
Online Resource
ISSN:
0041-1132
,
1537-2995
Language:
English
Publisher:
Wiley
Publication Date:
2023
detail.hit.zdb_id:
2018415-3
Permalink