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  • 1
    Online Resource
    Online Resource
    Kualitas dan Tingkat Maturasi Oosit Kucing Domestik dari Ovarium yang Disimpan dalam Waktu dan Media yang Berbeda
    Jurnal Veteriner ; 2021
    In:  Jurnal Veteriner Vol. 22, No. 3 ( 2021-09-30), p. 374-382
    Details
    In: Jurnal Veteriner, Jurnal Veteriner, Vol. 22, No. 3 ( 2021-09-30), p. 374-382
    Abstract: Penelitian ini bertujuan untuk mengevaluasi kualitas dan tingkat maturasi oosit kucing domestik yang disimpan dalam waktu dan media yang berbeda. Ovarium yang diperoleh setelah ovaryohisterectomy disimpan dalam tabung steril dan cara penyimpanannya dibagi menjadi tiga perlakuan , yaitu: 1) tanpa media, 2) berisi NaCl 0,9% atau 3) berisi PBS. Ovarium tersebut kemudian dibawa ke laboratorium dengan termos yang berisi NaCl 0,9% dengan suhu 35-37°C atau dengan cooler box suhu 4°C. Sampel ovarium suhu 4°C kemudian disimpan dalam refrigerator dengan suhu 4°C selama 24 dan 48 jam. Oosit dari ovarium yang dibawa dengan suhu suhu 35-37°C dikoleksi dalam waktu di bawah enam jam setelah sampai di laboratorium. Pada akhir penyimpanan, oosit dikoleksi dan dievaluasi kualitasnya. Selanjutnya, oosit dimaturasi dan dievaluasi tingkat maturasinya. Hasil dari penelitian ini menunjukkan bahwa berdasarkan morfologinya kualitas oosit kucing tidak dipengaruhi oleh waktu dan jenis media selama penyimpanan (P 〉 0,05). Tingkat maturasi oosit untuk mencapai tahap MII mulai menurun (P 〈 0,05) pada ovarium yang disimpan tanpa media maupun dengan PBS pada 24 jam periode penyimpanan, sedangkan oosit yang berasal dari ovarium yang disimpan dengan NaCl 0,9% mulai menurun (P 〈 0,05) pada 48 jam periode penyimpanan. Simpulan pada penelitian ini adalah penyimpanan ovarium dengan atau tanpa media selama 48 jam tidak memengaruhi morfologi oosit kucing namun memengaruhi tingkat maturasi oosit kucing.
    Type of Medium: Online Resource
    ISSN: 1411-8327 , 2477-5665
    Uniform Title: QUALITY AND MATURATION RATE OF DOMESTIC CAT OOCYTE FROM OVARY STORED IN DIFFERENT STORAGE TIME AND MEDIUM
    URL: Issue
    URL: Journal
    URL: Article
    DOI: 10.19087/jveteriner.2021.22.3
    DOI: 10.19087/jveteriner
    DOI: 10.19087/jveteriner.2021.22.3.374
    Language: Unknown
    Publisher: Jurnal Veteriner
    Publication Date: 2021
    detail.hit.zdb_id: 2911720-3
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  • 2
    Online Resource
    Online Resource
    Relationship between DNA fragmentation and nuclear status of in vitro-matured porcine oocytes: role of cumulus cells
    CSIRO Publishing ; 2004
    In:  Reproduction, Fertility and Development Vol. 16, No. 8 ( 2004), p. 773-
    Details
    In: Reproduction, Fertility and Development, CSIRO Publishing, Vol. 16, No. 8 ( 2004), p. 773-
    Abstract: The present study was conducted to investigate the effects of the attachment of cumulus cells to oocytes and coculture with cumulus cells during maturation culture on the nuclear status and DNA fragmentation of porcine denuded oocytes (DOs). In the first experiment, cumulus cells were removed from cumulus–oocyte complexes (COCs) at 0, 8, 16, 24 or 32 h after the onset of maturation culture and the DOs were then cultured in their original droplets until 42 h of culture was reached. In the second experiment, all COCs were denuded before the onset of culture and the DOs were cocultured with their removed cumulus cells. The DOs were transferred into fresh medium at 0, 8, 16, 24 or 32 h after the onset of coculture with cumulus cells and then cultured until 42 h of culture was reached. After culture, DNA fragmentation and the nuclear status of oocytes were examined using the terminal deoxyribonucleotidyl transferase-mediated dUTP–digoxigenin nick end-labelling (TUNEL) method. When the DOs were returned to the same droplets after removal of the cumulus cells, the removal of the cumulus cells after 16 h of culture significantly decreased the proportion of oocytes remaining at the germinal vesicle (GV) stage. However, coculture treatment of DOs in the presence of their removed cumulus cells had no significant effects on the GV breakdown (GVBD) of oocytes. There were no significant differences in the proportion maturing to MII oocytes among the groups following removal of cumulus cells after the onset of maturation culture; however, DOs cocultured with cumulus cells until the end of maturation culture exhibited an increased maturation rate compared with DOs cocultured for 8 and 16 h. The total proportion of TUNEL-positive oocytes of oocytes remaining at the GV stage was higher than that of oocytes reaching other stages, irrespective of the removal of cumulus cells and coculture treatments. However, coculture for more than 16 h decreased the total proportion of TUNEL-positive oocytes. Our results indicate that the attachment of cumulus cells to oocytes may have a critical role for oocytes undergoing GVBD and that coculture with cumulus cells promotes the ability of oocytes to complete maturation. Moreover, coculture with cumulus cells may assist the oocyte to avoid undergoing DNA fragmentation.
    Type of Medium: Online Resource
    ISSN: 1031-3613
    URL: Article
    DOI: 10.1071/RD03099
    Language: English
    Publisher: CSIRO Publishing
    Publication Date: 2004
    SSG: 12
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  • 3
    Online Resource
    Online Resource
    TINGKAT PERKEMBANGAN AWAL EMBRIO SAPI IN VITRO MENGGUNAKAN MEDIA TUNGGAL BERBAHAN DASAR TISSUE CULTURE MEDIUM (TCM) 199
    LPPM Unsyiah ; 2013
    In:  Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences Vol. 7, No. 2 ( 2013-09-01)
    Details
    In: Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences, LPPM Unsyiah, Vol. 7, No. 2 ( 2013-09-01)
    Abstract: Penelitian ini bertujuan mengetahui kemampuan perkembangan awal embrio sapi in vitro menggunakan media tunggal untuk maturasi, fertilisasi, dan kultur berbahan dasar tissue culture medium (TCM) 199. Oosit sapi dikumpulkan dari rumah potong hewan dengan teknik aspirasi dan diklasifikasikan berdasarkan kekompakan sel kumulus dan sitoplasma yang homogen. Oosit dimaturasi pada medium TCM 199 yang disuplementasi dengan 10 IU/ml pregnant mare’s serum gonadotropin (PMSG), 10 IU/ml human chorionic gonadotropin (hCG), dan 10% fetal bovine serum (FBS), dilakukan selama 24 jam pada inkubator 5% CO2, 39 C. Fertilisasi dilakukan pada dua media yang berbeda yaitu media rutin fertilisasi dan media berbahan dasar TCM 199 dengan suplemen bovine serum albumin (BSA) dan heparin. Setelah fertilisasi, kumulus sel dihilangkan (denudasi), kemudian dikultur pada media TCM 199 yang disuplementasi dengan asam amino esensial dan non-esensial serta 10% FBS selama 3 hari. Hasil penelitian menunjukkan tingkat maturasi oosit pada sistem yang digunakan mampu mendukung 81,5% oosit mencapai tahap metafase II (M-II). Tingkat pembelahan embrio lebih tinggi pada media rutin dibandingkan dengan media TCM 199 yakni masing-masing 44,4 dan 23,2%. Jumlah embrio tahap 4-8 sel pada kedua perlakuan tidak berbeda nyata. Dapat disimpulkan media tunggal berbasis TCM dapat digunakan untuk produksi embrio in vitro.
    Type of Medium: Online Resource
    ISSN: 2502-5600 , 1978-225X
    URL: Article
    DOI: 10.21157/j.ked.hewan.v7i2.930
    Language: Unknown
    Publisher: LPPM Unsyiah
    Publication Date: 2013
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  • 4
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    Online Resource
    Relationship between Nucleus Swelling and Development Competence of Bovine Cloned Embryos Reconstructed by Enucleated Oocytes with Serum-starved or Serum-fed Fetal Somatic Cells
    Universitas Gadjah Mada ; 2015
    In:  Indonesian Journal of Biotechnology Vol. 10, No. 2 ( 2015-10-13)
    Details
    In: Indonesian Journal of Biotechnology, Universitas Gadjah Mada, Vol. 10, No. 2 ( 2015-10-13)
    Abstract: This study was conducted to examine the occurrence of nuclear remodeling (nucleus swelling) and its effectson the subsequent in vitro development of bovine embryos reconstructed by serum-starved and serum-fed somaticcells. Results from this study demonstrated that all of the reconstructed embryos that received serum-starved andserum-fed somatic cells exhibited condensed-nuclei. More than 90% of the transferred nuclei exhibited nuclearenvelope breakdown and premature chromatin condensation which clearly distinct from an intact nucleus. Therewas no significant difference on the degree of nucleus swelling in SS-NT embryos or SF-NT embryos, indicatingthat either serum-starved or confluent somatic cell lines could be reprogrammed by the recipient cytoplasmenvironments in similar pattern. Although the fusion rate was not significantly different among the groups, theproportion of SS-NT embryos which developed to the 2- to 4-cell stage (89.7%) and to the 8- to 16-cell stage (74.7%)was significantly higher than that of SF-NT embryos. Whereas, the proportion of reconstructed embryos thatdeveloped to the morula and blastocyst stages were not significantly different among the groups. Results of thesestudies demonstrate that reconstructed embryos, which received either serum-starved or serum-fed confluentsomatic cells, showed similar developmental competence to the blastocyst stage.Keywords: nuclear transplantation technique-somatic cells-nucleus swelling
    Type of Medium: Online Resource
    ISSN: 2089-2241 , 0853-8654
    URL: Article
    DOI: 10.22146/ijbiotech.7553
    Language: Unknown
    Publisher: Universitas Gadjah Mada
    Publication Date: 2015
    detail.hit.zdb_id: 2705379-9
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  • 5
    Online Resource
    Online Resource
    Effects of Ovary Storage Time and Temperature on DNA Fragmentation and Development of Porcine Oocytes
    Japanese Society of Animal Reproduction ; 2005
    In:  Journal of Reproduction and Development Vol. 51, No. 1 ( 2005), p. 87-97
    Details
    In: Journal of Reproduction and Development, Japanese Society of Animal Reproduction, Vol. 51, No. 1 ( 2005), p. 87-97
    Type of Medium: Online Resource
    ISSN: 0916-8818 , 1348-4400
    URL: Article
    DOI: 10.1262/jrd.51.87
    Language: English
    Publisher: Japanese Society of Animal Reproduction
    Publication Date: 2005
    detail.hit.zdb_id: 2039060-9
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  • 6
    Online Resource
    Online Resource
    Developmental Competence of Cat Oocytes from Ovaries Stored at Various Temperature for 24 h
    Japanese Society of Animal Reproduction ; 2007
    In:  Journal of Reproduction and Development Vol. 53, No. 2 ( 2007), p. 271-277
    Details
    In: Journal of Reproduction and Development, Japanese Society of Animal Reproduction, Vol. 53, No. 2 ( 2007), p. 271-277
    Type of Medium: Online Resource
    ISSN: 0916-8818 , 1348-4400
    URL: Article
    DOI: 10.1262/jrd.18115
    Language: English
    Publisher: Japanese Society of Animal Reproduction
    Publication Date: 2007
    detail.hit.zdb_id: 2039060-9
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  • 7
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    Online Resource
    Diploid porcine parthenotes produced by inhibition of first polar body extrusion during in vitro maturation of follicular oocytes
    Bioscientifica ; 2006
    In:  Reproduction Vol. 132, No. 4 ( 2006-10), p. 559-570
    Details
    In: Reproduction, Bioscientifica, Vol. 132, No. 4 ( 2006-10), p. 559-570
    Abstract: We investigated nuclear progression and in vitro embryonic development after parthenogenetic activation of porcine oocytes exposed to cytochalasin B (CB) during in vitro maturation (IVM). Nuclear progression was similar in control oocytes and oocytes matured in the presence of 1 μg/ml CB (IVM-CB group) by 37 h IVM; at this time the proportion of oocytes that had reached or passed through the anaphase-I stage did not differ significantly between the IVM-CB and the control groups (61.3 and 69.9% respectively; P 〈 0.05). After IVM for 37 h, no polar body extrusion was observed in the IVM-CB group. In these oocytes, the two lumps of homologous chromosomes remained in the ooplasm after their segregation and turned into two irregular sets of condensed chromosomes. By 41 h IVM, the double sets of chromosomes had reunited in 89.5% IVM-CB oocytes and formed a single large metaphase plate, whereas 68.8% of the control oocytes had reached the metaphase-II stage by this time. When IVM-CB oocytes cultured for 46 h were stimulated with an electrical pulse and subsequently cultured for 8 h without CB, 39.0% of them extruded a polar body and 82.9% of them had a female pronucleus. Chromosome analysis revealed that the majority of oocytes that extruded a polar body were diploid in both the control and the IVM-CB groups. However, the incidence of polyploidy in the IVM-CB group was higher than that in the control group ( P 〈 0.05). In vitro development of diploid parthenotes in the control and the IVM-CB groups was similar in terms of blastocyst formation rates (45.8 and 42.8% respectively), number of blastomeres (39.9 and 44.4 respectively), the percentage of dead cells (4.3 and 2.9% respectively), and the frequency of apoptotic cells (7.3 and 6.3% respectively). Tetraploid embryos had a lower blastocyst formation rate (25.5%) and number of cells (26.2); however, the proportion of apoptotic nuclei (7.0%) was similar to that in diploid parthenotes. These results suggest that the proportion of homozygous and heterozygous genes does not affect in vitro embryo development to the blastocyst stage.
    Type of Medium: Online Resource
    ISSN: 1470-1626 , 1741-7899
    URL: Article
    DOI: 10.1530/rep.1.01216
    Language: Unknown
    Publisher: Bioscientifica
    Publication Date: 2006
    detail.hit.zdb_id: 2037813-0
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  • 8
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    Online Resource
    PRM1 Gene Expression and Its Protein Abundance in Frozen-Thawed Spermatozoa as Potential Fertility Markers in Breeding Bulls
    MDPI AG ; 2022
    In:  Veterinary Sciences Vol. 9, No. 3 ( 2022-03-03), p. 111-
    Details
    In: Veterinary Sciences, MDPI AG, Vol. 9, No. 3 ( 2022-03-03), p. 111-
    Abstract: Functional genes and proteins in sperm play an essential role in bulls’ reproductive processes. They are more accurate in determining bull fertility than conventional semen quality tests. Protamine-1 (PRM1) is a gene or protein crucial for packaging and protecting sperm DNA until fertilization affects normal sperm function. This study analyzes the genes and proteins potential from PRM1 as fertility markers for different breeds of bulls utilized in the artificial insemination programs, expected to be an accurate tool in interpreting bull fertility in Indonesia. This study used Limousin, Holstein, and Ongole Grade bulls divided into two groups based on fertility, high-fertility (HF) and low fertility (LF). The semen quality assessment included progressive motility (computer-assisted semen analysis), viability (eosin-nigrosine), and plasma membrane integrity (HOS test). Sperm DNA fragmentation (SDF) was assessed using the acridine orange staining and the Halomax test. Sperm PRM deficiency was evaluated with the chromomycin A3 method. Moreover, PRM1 gene expression was measured using qRT-PCR, and the PRM1 protein abundance was measured with the enzyme immunoassay method. Semen quality values, relative expression of PRM1 gene, and quantity of PRM1 protein were significantly higher (p 〈 0.05) in HF bulls than in LF bulls. The SDF and PRM deficiency values in LF bulls were significantly higher (p 〈 0.05) than HF bulls. Additionally, PRM1 at the gene and protein levels correlated significantly (p 〈 0.01) with fertility. Therefore, PRM1 is a potential candidate for fertility markers in bulls in Indonesia.
    Type of Medium: Online Resource
    ISSN: 2306-7381
    URL: Article
    DOI: 10.3390/vetsci9030111
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2768971-2
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  • 9
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    Online Resource
    Adiponectin: Potential Protein Hormone as a Candidate Biomarker for Male Fertility
    Indonesian Center for Animal Research and Development (ICARD) ; 2019
    In:  Indonesian Bulletin of Animal and Veterinary Sciences Vol. 29, No. 4 ( 2019-12-07), p. 183-
    Details
    In: Indonesian Bulletin of Animal and Veterinary Sciences, Indonesian Center for Animal Research and Development (ICARD), Vol. 29, No. 4 ( 2019-12-07), p. 183-
    Abstract: Reproduction is an important factor that requires attention to increase livestock production. The application of artificial insemination (AI) technology has been developed rapidly in the world, so that availability of fertile superior male is a determinant factor of successful AI. Several recent studies have been aimed to discover male fertility biomarkers, by intense research on fertility-assosiated proteins contained in seminal plasma. This paper describes the role of adiponectin as a candidate of male biomarker fertility. Adiponectin has a positive effect on spermatozoa functionality and steroidogenesis. This has been proven by several studies that found its expression in Sertoli cells and Leydig cells in the reproductive tract. Based on the specific role on spermatozoa functionality and steroidogenesis in supporting male fertility parameter, it is strongly suggested that adiponectin is an excellent candidate biomarker for male fertility.
    Type of Medium: Online Resource
    ISSN: 2354-6832 , 0216-6461
    URL: Article
    DOI: 10.14334/wartazoa.v29i4.2063
    Language: Unknown
    Publisher: Indonesian Center for Animal Research and Development (ICARD)
    Publication Date: 2019
    detail.hit.zdb_id: 2853339-2
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  • 10
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    Breed and age effects on concentration of adiponectin and reproductive performance in Anglo Nubian, Etawah grade and its crossbred bucks
    UNS Solo ; 2021
    In:  Biodiversitas Journal of Biological Diversity Vol. 22, No. 3 ( 2021-02-08)
    Details
    In: Biodiversitas Journal of Biological Diversity, UNS Solo, Vol. 22, No. 3 ( 2021-02-08)
    Abstract: Abstract. Hafizuddin, Karja NWK, Praharani L, Setiadi MA. 2021. Breed and age effects on concentration of adiponectin and reproductive performance in Anglo Nubian, Etawah grade and its crossbred bucks. Biodiversitas 22: 1112-1119. The purpose of this study was to evaluate the effect of differences in breed and age on reproductive performance and adiponectin and testosterone production in Anglo Nubian, Etawah grade, and crossbred (Anpera) bucks. A total of 12 bucks with four individuals of each breed were used. This study collected five data points from each buck regarding adiponectin, testosterone, and reproductive performance (libido and semen characteristics). Data were analyzed with factorial analysis of variance and Duncan's test. The result shows that adiponectin concentration between breeds was significantly different (P 〈 0.01). There were also significant differences (P 〈 0.05) in adiponectin concentrations based on buck age. There were also significant interactions with adiponectin concentrations (P 〈 0.01). Furthermore, testosterone concentrations showed significant differences based on breed (P 〈 0.05) and age (P 〈 0.05). There were also significant age-breed interactions affecting testosterone concentrations (P 〈 0.01). Libido and semen characteristics had no significant differences based on breed and age group, and no significant age-breed interactions (P 〉 0.05). The heterosis effect on adiponectin concentration (48.05%), testosterone concentration (27.68%), libido (-0.61%), semen volume (-1.93%), sperm motility (0.49%), sperm normal morphology (0.18%), and sperm concentration (0.00%) was measured. In conclusion, there is a significant effect of breed, age, and age-breed interactions on the concentration of adiponectin and testosterone in bucks, and both of these variables have a high heterosis effect on crossbred bucks.
    Type of Medium: Online Resource
    ISSN: 2085-4722 , 1412-033X
    URL: Article
    DOI: 10.13057/biodiv/d220305
    Language: Unknown
    Publisher: UNS Solo
    Publication Date: 2021
    detail.hit.zdb_id: 2660049-3
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