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  • 1
    Online Resource
    Online Resource
    Induction of CYP2E1 in testes of isoniazid-treated rats as possible cause of testicular disorders
    Elsevier BV ; 2015
    In:  Toxicology Letters Vol. 234, No. 2 ( 2015-04), p. 59-66
    Details
    In: Toxicology Letters, Elsevier BV, Vol. 234, No. 2 ( 2015-04), p. 59-66
    Type of Medium: Online Resource
    ISSN: 0378-4274
    URL: Article
    DOI: 10.1016/j.toxlet.2015.02.008
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2015
    detail.hit.zdb_id: 1500784-4
    SSG: 12
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  • 2
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    Chronic alcoholism-mediated metabolic disorders in albino rat testes
    Walter de Gruyter GmbH ; 2014
    In:  Interdisciplinary Toxicology Vol. 7, No. 3 ( 2014-9-1), p. 165-172
    Details
    In: Interdisciplinary Toxicology, Walter de Gruyter GmbH, Vol. 7, No. 3 ( 2014-9-1), p. 165-172
    Abstract: There is good evidence for impairment of spermatogenesis and reductions in sperm counts and testosterone levels in chronic alcoholics. The mechanisms for these effects have not yet been studied in detail. The consequences of chronic alcohol consumption on the structure and/or metabolism of testis cell macromolecules require to be intensively investigated. The present work reports the effects of chronic alcoholism on contents of free amino acids, levels of cytochrome P450 3A2 (CYP3A2) mRNA expression and DNA fragmentation, as well as on contents of different cholesterol fractions and protein thiol groups in rat testes. Wistar albino male rats were divided into two groups: I - control (intact animals), II - chronic alcoholism (15% ethanol self-administration during 150 days). Following 150 days of alcohol consumption, testicular free amino acid content was found to be significantly changed as compared with control. The most profound changes were registered for contents of lysine (-53%) and methionine (+133%). The intensity of DNA fragmentation in alcohol-treated rat testes was considerably increased, on the contrary CYP3A2 mRNA expression in testis cells was inhibited, testicular contents of total and etherified cholesterol increased by 25% and 45% respectively, and protein SH-groups decreased by 13%. Multidirectional changes of the activities of testicular dehydrogenases were detected. We thus obtained complex assessment of chronic alcoholism effects in male gonads, affecting especially amino acid, protein, ATP and NADPH metabolism. Our results demonstrated profound changes in testes on the level of proteome and genome. We suggest that the revealed metabolic disorders can have negative implication on cellular regulation of spermatogenesis under long-term ethanol exposure.
    Type of Medium: Online Resource
    ISSN: 1337-9569
    URL: Article
    DOI: 10.2478/intox-2014-0023
    Language: English
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2014
    detail.hit.zdb_id: 2503775-4
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  • 3
    Online Resource
    Online Resource
    Multiparameter rodent chronic model for complex evaluation of alcoholism-mediated metabolic violations
    Walter de Gruyter GmbH ; 2015
    In:  Journal of Basic and Clinical Physiology and Pharmacology Vol. 26, No. 1 ( 2015-01-1)
    Details
    In: Journal of Basic and Clinical Physiology and Pharmacology, Walter de Gruyter GmbH, Vol. 26, No. 1 ( 2015-01-1)
    Abstract: Despite of the wide spectrum of alcoholism experimental models, the majority of them are very specialized on the short list of investigated parameters and could not provide reproduction of complex metabolic changes in the rats. The aim of the present study was to estimate whether rats selected by high alcohol preference, allowed free access to 15% alcohol for 150 days, develop simultaneous multilevel disturbances of cell macromolecules structure, metabolism and oxidative/nitrosative stress. Wistar albino male rats were divided into groups: I – rats selected by preferences to alcohol were used for chronic alcoholism modeling by replacing water with 15% ethanol (150 days), II – control. Contents of amino acids in serum, liver mRNA CYP2E1 and CYP3A2 expression, DNA fragmentation and lipid peroxidation levels, the reduced glutathione content, superoxide dismutase, catalase, iNOS and cNOS activities were evaluated. In serum of ethanol-treated rats contents of aspartic acid, serine, glycine, alanine and valine were decreased whereas contents of histidine, methionine and phenylalanine were increased. Liver CYP2E1, CYP3A2 mRNA expression, DNA fragmentation levels significantly elevated. Level of cNOS in ethanol-treated rat’s hepatocytes was within the normal limits, whereas iNOS activity was raised 1.6 times. Liver pro- and anti-oxidant system alterations were shown. Rats’ chronic 15% alcohol consumption (150 days) led solely to complex metabolomic changes at different levels, which simultaneously characterized cell macromolecules structure, metabolism, and oxidative/nitrosative stress. Rodent model of chronic alcoholism in the proposed modification could be an adequate and reasonably priced tool for further preclinical development and testing of pharmacotherapeutic agents.
    Type of Medium: Online Resource
    ISSN: 2191-0286 , 0792-6855
    URL: Article
    DOI: 10.1515/jbcpp-2013-0163
    Language: Unknown
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2015
    detail.hit.zdb_id: 2602428-7
    SSG: 15,3
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  • 4
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    Online Resource
    Reproductive Disorders in Streptozotocin-Treated Male Rats Following Co-Administration of Ethambutol, Rifampicin, Isoniazid and Pyrazinamide
    Walter de Gruyter GmbH ; 2012
    In:  Romanian Journal of Diabetes Nutrition and Metabolic Diseases Vol. 19, No. 4 ( 2012-12-1), p. 405-415
    Details
    In: Romanian Journal of Diabetes Nutrition and Metabolic Diseases, Walter de Gruyter GmbH, Vol. 19, No. 4 ( 2012-12-1), p. 405-415
    Abstract: Objectives: To evaluate the effect of anti-tuberculosis drugs (ATD) on indices of reproductive capability, DNA fragmentation and offspring development of male rats with testicular malfunction caused by experimental diabetes. Materials and Methods: Wistar albino male rats (body weight 160-200 g) were divided into three groups: I - control, II - streptozotocin diabetes, III - streptozotocin diabetes + ATD. The testis DNA fragmentation was determined electrophoretically; spermatogenetic indices, offspring antenatal and postnatal development indices - by standard procedures. Morphological analyses of gonadic structures were carried out by optic microscopy. Results: The study of the effects of diabetes and ATD administration on testis cells morphologic and morphometric parameters and spermatogenesis suggested the presence of specific diabetes- and anti tuberculosis drugs - mediated quantitative and qualitative changes in male rat reproductive organs, spermatogenetic epithelial cells, level and character of DNA fragmentation in comparison with normal rats. These changes were accompanied by alterations in processes of fertilisation (with intact females), embryogenesis and by lowering of offspring survival. Conclusions: Observed changes could hence affect the state and correct functioning of spermatogenetic epithelium and of other tissues of reproductive organs, as well as offspring development in diabetic rats.
    Type of Medium: Online Resource
    ISSN: 2284-6417 , 2068-8245
    URL: Article
    DOI: 10.2478/v10255-012-0047-8
    Language: Unknown
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2012
    detail.hit.zdb_id: 2860636-X
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  • 5
    Online Resource
    Online Resource
    Diabetes-mediated changes in rat type i collagen and spermatogenesis indices
    Walter de Gruyter GmbH ; 2012
    In:  Romanian Journal of Diabetes Nutrition and Metabolic Diseases Vol. 19, No. 3 ( 2012-10-1), p. 245-254
    Details
    In: Romanian Journal of Diabetes Nutrition and Metabolic Diseases, Walter de Gruyter GmbH, Vol. 19, No. 3 ( 2012-10-1), p. 245-254
    Abstract: Objectives: To investigate the effects of diabetes on the reproductive system and extracellular matrix proteins of diabetic rats. Materials and Methods: Wistar albino male rats, body weight (BW) 160-200 g, were divided into two groups: I - streptozoticin diabetes, II - normal non-diabetic animals. The content of amino acids in rat type I collagen was determined using an amino acid analyzer. Morphological analyses of gonadic structures were carried out by an optic microscope. Results: The study of the effects of diabetes on type I collagen amino acid content, testis cells morphologic and morphometric parameters and spermatogenesis demonstrated the presence of diabetes-mediated quantitative and qualitative changes in male rat reproductive organs, spermatogenetic epithelial cells and extracellular matrix proteins in comparison with normal. Conclusions: Observed collagen molecules changes could hence affect the properties and correct functioning of spermatogenetic epithelium and of other tissues of reproductive organs. They could be caused by diabetes via deficiency of insulin which is involved in collagen synthesis regulation at different stages of this process, cytochrome P450-2E1 induction and reactive oxygen species effects on protein biosynthesis processes.
    Type of Medium: Online Resource
    ISSN: 2284-6417 , 2068-8245
    URL: Article
    DOI: 10.2478/v10255-012-0030-4
    Language: Unknown
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2012
    detail.hit.zdb_id: 2860636-X
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  • 6
    Online Resource
    Online Resource
    CYP2E1 Testis Expression and Alcoholmediated Changes of Rat Spermatogenesis Indices and Type I Collagen
    Walter de Gruyter GmbH ; 2013
    In:  Archives of Industrial Hygiene and Toxicology Vol. 64, No. 2 ( 2013-06-1), p. 237-246
    Details
    In: Archives of Industrial Hygiene and Toxicology, Walter de Gruyter GmbH, Vol. 64, No. 2 ( 2013-06-1), p. 237-246
    Abstract: This study is a complex investigation of alcohol-mediated changes in CYP2E1 mRNA and protein expression in the testes, as well as spermatogenesis indices and type I collagen amino acid contents, in male rats. Wistar albino male rats were divided into two groups: I - control (intact animals), II - experimental (chronic alcoholism, exposure to a 15 % ethanol aqueous solution during 150 days). The destructive changes in the spermatogenic epithelium were accompanied by a decrease in sperm number and motility time. CYP2E1 mRNA and protein expression were elevated in the testes 3 and 1.4 times, respectively. Also, significantly lower contents of lysine, glutamic acid, serine, proline, alanine, valine, and phenylalanine residues accompanied by an increase of hydroxyproline, glycine, and threonine residue contents were detected in the skin type I collagen of the experimental group. Chronic ethanol consumption caused testicular failure along with an overexpression of CYP2E1 mRNA and protein in the testes as well as quantitative changes in type I collagen amino acid contents. The profound alcohol-mediated changes in collagen type I amino acid contents may have affected the spermatogenic epithelium state. The modulation of testicular cytochrome P450 2E1 mRNA and protein expression could change the functioning of this isozyme in target organs and take part in the mechanism of ethanol gonadotoxicity.
    Type of Medium: Online Resource
    ISSN: 0004-1254
    URL: Article
    DOI: 10.2478/10004-1254-64-2013-2313
    Language: Unknown
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2013
    detail.hit.zdb_id: 2262350-4
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  • 7
    Online Resource
    Online Resource
    Comparative investigation of methionine and novel formulation Metovitan protective effects in Wistar rats with testicular and epididymal toxicity induced by anti-tuberculosis drugs co-administration
    Elsevier BV ; 2017
    In:  Food and Chemical Toxicology Vol. 99 ( 2017-01), p. 222-230
    Details
    In: Food and Chemical Toxicology, Elsevier BV, Vol. 99 ( 2017-01), p. 222-230
    Type of Medium: Online Resource
    ISSN: 0278-6915
    URL: Article
    DOI: 10.1016/j.fct.2016.12.001
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2017
    detail.hit.zdb_id: 1483645-2
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  • 8
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    Reproductive disorders in male rats induced by high-fructose consumption from juvenile age to puberty
    Walter de Gruyter GmbH ; 2020
    In:  Archives of Industrial Hygiene and Toxicology Vol. 71, No. 1 ( 2020-03-01), p. 78-86
    Details
    In: Archives of Industrial Hygiene and Toxicology, Walter de Gruyter GmbH, Vol. 71, No. 1 ( 2020-03-01), p. 78-86
    Abstract: There is compelling evidence that a hypercaloric, high-fructose diet can cause metabolic syndrome (MetS) and a whole range of other metabolic changes. In the context of androgen deficiency, MetS in boys merits special attention, but the effects of fructose-rich diet in youth on future male reproductive function are still poorly evidenced. The aim of this study was to address this issue and analyse the effects of high-fructose intake starting from weaning to puberty (postnatal day 23 up to 83) on the reproductive function of male rats. For this purpose juvenile male Wistar rats were divided in two groups: control and the group receiving 10 % fructose solution instead of drinking water. Reproductive function was evaluated in terms of fertility, sperm count, testes/epididymis morphology, and serum sex hormones. The fructose-treated group showed a decrease in testosterone and twofold increase in luteinising and follicle-stimulating hormone levels in the serum. This was accompanied with lower testis/epididymis weights, sperm count, and changed testis/epididymis morphology. Their fertility remained unchanged, but the fertility of females mating with these males diminished. In addition, pre-implantation and post-implantation embryonic death rate rose in these females. Our results have confirmed that high fructose consumption from early age until puberty can impair the reproductive function of male rats, and call for further animal and epidemiological investigation.
    Type of Medium: Online Resource
    ISSN: 1848-6312
    URL: Article
    DOI: 10.2478/aiht-2020-71-3303
    Language: English
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2020
    detail.hit.zdb_id: 2262350-4
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  • 9
    Online Resource
    Online Resource
    Correlation between spermatogenesis disorders and rat testes CYP2E1 mRNA contents under experimental alcoholism or type I diabetes
    Elsevier BV ; 2014
    In:  Advances in Medical Sciences Vol. 59, No. 2 ( 2014-09), p. 183-189
    Details
    In: Advances in Medical Sciences, Elsevier BV, Vol. 59, No. 2 ( 2014-09), p. 183-189
    Type of Medium: Online Resource
    ISSN: 1896-1126
    URL: Article
    DOI: 10.1016/j.advms.2014.03.004
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2014
    detail.hit.zdb_id: 2273671-2
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  • 10
    Online Resource
    Online Resource
    Age-dependent features of CYP3A, CYP2C, and CYP2E1 functioning at metabolic syndrome
    Walter de Gruyter GmbH ; 2016
    In:  Journal of Basic and Clinical Physiology and Pharmacology Vol. 27, No. 6 ( 2016-01-1)
    Details
    In: Journal of Basic and Clinical Physiology and Pharmacology, Walter de Gruyter GmbH, Vol. 27, No. 6 ( 2016-01-1)
    Abstract: Complex investigations of cytochrome P450 (CYP) isoforms with metabolic syndrome (MS) development are limited, and specific features of adolescent’s metabolisms are generally disregarded. The aim of present study was a comparative estimation of MS-mediated changes in CYP3A, CYP2C, and CYP2E1 mRNA expression and enzymatic activities, as well as antioxidant system parameters of adult and pubertal rats. Methods: Wistar albino male rats of two age categories [young animals of 21 days age (50–70 g) and adults (160–180 g)] were divided into four groups (eight animals in each group): (1) control 1 (intact young rats), (2) control 2 (intact adult rats), (3) MS3 (young rats with MS), and (4) MS4 (adult rats with MS). The MS was induced by full replacement of drinking water by 20% fructose solution (200 g/L). After 60 days of MS modeling, the investigation of rat liver CYP3A, CYP2C, and CYP2E1 mRNA expressions, their enzyme-marker activities, as well as the antioxidant system parameters was conducted. Results: Levels of liver CYP2E1 mRNA expression increased with MS: 40% (adults) and 80% (pubertal rats). Pubertal rats had also increased CYP3A2 mRNA expression (30%) and decreased CYP2C mRNA expression (30%). Changes in CYP2E1 and CYP2C enzymatic activities were consistent with the changes of corresponding gene expressions at both age-groups with MS. Simultaneously, liver reduced glutathione contents, and glutathione transferase and reductase activities were decreased in pubertal animals. Conclusions: CYP isoform expression rates and glutathione system were greatly violated with MS. The greater changes were observed in pubertal rats with MS.
    Type of Medium: Online Resource
    ISSN: 2191-0286 , 0792-6855
    URL: Article
    DOI: 10.1515/jbcpp-2016-0012
    Language: Unknown
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2016
    detail.hit.zdb_id: 2602428-7
    SSG: 15,3
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