In:
Macromolecular Bioscience, Wiley, Vol. 4, No. 8 ( 2004-08-09), p. 766-775
Abstract:
Summary: We report on a novel series of biomimetic polymers exhibiting interfacial properties similar to the extracellular matrix. A series of well‐defined surfactant polymers were synthesized by simultaneously incorporating arginine‐glycine‐aspartic acid (RGD) peptide, dextran oligosaccharide, and hexyl ligands with controlled feed ratios onto a poly(vinyl amine) (PVAm) backbone. The peptide sequence was H‐GSSSG RGD SPA‐NH 2 (Pep) having a hydrophilic extender at the amino terminus and capped carboxy terminus. The peptide‐to‐dextran (Pep:Dex) ratios were varied to create surfactants having 0, 25, 50, 75, and 100 mol‐% peptide relative to dextran. The surfactants were characterized by IR, NMR and atomic force microscopy (AFM) for composition and surface active properties. AFM confirmed full surface coverage of PVAm(Pep)(100%) on graphite, and supported the mechanism of interdigitation of hexyl ligands between surfactant molecules within a specified range of hexyl chain densities. the attachment and growth of human pulmonary artery endothelial cells on the PVAm(Pep)(100%) surface was identical to the fibronectin positive control. Cell adhesion decreased dramatically with decreasing peptide density on the surfactant polymers. Molecular model of a peptide surfactant polymer, consisting of poly(vinyl amine) backbone with peptide, dextran oligosaccharide and hexyl branches coupled to the polymer chain. magnified image Molecular model of a peptide surfactant polymer, consisting of poly(vinyl amine) backbone with peptide, dextran oligosaccharide and hexyl branches coupled to the polymer chain.
Type of Medium:
Online Resource
ISSN:
1616-5187
,
1616-5195
DOI:
10.1002/mabi.200300125
Language:
English
Publisher:
Wiley
Publication Date:
2004
detail.hit.zdb_id:
2039130-4
detail.hit.zdb_id:
2041797-4
SSG:
12
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