In:
The Journal of Neuroscience, Society for Neuroscience, Vol. 17, No. 4 ( 1997-02-15), p. 1350-1362
Abstract:
Cerebellar granule cells express six GABA A receptor subunits abundantly (α 1 , α 6 , β 2 , β 3 , γ 2 , and δ) and assemble various pentameric receptor subtypes with unknown subunit compositions; however, the rules guiding receptor subunit assembly are unclear. Here, removal of intact α 6 protein from cerebellar granule cells allowed perturbations in other subunit levels to be studied. Exon 8 of the mouse α 6 subunit gene was disrupted by homologous recombination. In α 6 −/− granule cells, the δ subunit was selectively degraded as seen by immunoprecipitation, immunocytochemistry, and immunoblot analysis with δ subunit-specific antibodies. The δ subunit mRNA was present at wild-type levels in the mutant granule cells, indicating a post-translational loss of the δ subunit. These results provide genetic evidence for a specific association between the α 6 and δ subunits. Because in α 6 −/− neurons the remaining α 1 , β 2/3 , and γ 2 subunits cannot rescue the δ subunit, certain potential subunit combinations may not be found in wild-type cells.
Type of Medium:
Online Resource
ISSN:
0270-6474
,
1529-2401
DOI:
10.1523/JNEUROSCI.17-04-01350.1997
Language:
English
Publisher:
Society for Neuroscience
Publication Date:
1997
detail.hit.zdb_id:
1475274-8
SSG:
12
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