In:
Mycoses, Wiley, Vol. 61, No. 12 ( 2018-12), p. 938-944
Kurzfassung:
Dihydroxyacid dehydratase ( DHAD ) is a key enzyme in biosynthetic pathway of isoleucine and valine. This pathway is absent in human but exists in various organisms such as fungi. Using RNA ‐seq analysis in this study, we identified MGL _3741 gene which encodes DHAD protein in Malassezia globosa ( M. globosa ). Furthermore, we found that mentioned gene is homologous to the Ustilago maydis , Saccharomyces cerevisiae , Aspergillus flavus , and Aspergillus fumigatus ILV 3P. For understanding the probable role of this gene in pathogenicity of M. globosa, we applied Real‐time PCR to investigate the differentially expressed of the MGL _3741 gene in healthy and pathogenic states. Our results indicate a significant difference between two mentioned stats. These results revealed that ILV 3 ‐like gene in M. globosa can be related to the pathogenicity of this yeast.
Materialart:
Online-Ressource
ISSN:
0933-7407
,
1439-0507
DOI:
10.1111/myc.2018.61.issue-12
Sprache:
Englisch
Verlag:
Wiley
Publikationsdatum:
2018
ZDB Id:
2020780-3
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