In:
Genes to Cells, Wiley, Vol. 11, No. 7 ( 2006-07), p. 701-717
Abstract:
Two major apoptotic signaling pathways have been defined in mammals, the extrinsic pathway, initiated by ligation of death receptors, and the intrinsic pathway, triggered by cytochrome c release from mitochondria. Here, we identified and characterized the Xenopus homologs of caspase‐10 (xCaspase‐10β), a novel initiator caspase, and Bid (xBid), a BH3‐only molecule of the Bcl‐2 family involved in both the extrinsic and intrinsic pathways. Exogenous expression of these molecules induced apoptosis of mammalian cells. By biochemical and cytological analyses, we clarified that xCaspase‐10β and xBid exhibit structural and functional similarities to their mammalian orthologues. We also detected xCaspase‐10β and xBid transcripts during embryogenesis by whole‐mount in situ hybridization and RT‐PCR analysis. Microinjection of mRNA encoding a protease‐defect xCaspase‐10β mutant into embryos resulted in irregular development. Enforced expression of active xBid induced cell death in developing embryos. Using transgenic frogs established to allow monitoring of caspase activation in vivo, we confirmed that this form of cell death is caspase‐dependent apoptosis. Thus, we demonstrated that the machinery governing the extrinsic and intrinsic apoptotic pathways are already established in Xenopus embryos. Additionally, we propose that the functions of the initiator caspase and BH3‐only molecule are evolutionarily conserved in vertebrates, functioning during embryonic development.
Type of Medium:
Online Resource
ISSN:
1356-9597
,
1365-2443
DOI:
10.1111/gtc.2006.11.issue-7
DOI:
10.1111/j.1365-2443.2006.00983.x
Language:
English
Publisher:
Wiley
Publication Date:
2006
detail.hit.zdb_id:
2020308-1
SSG:
12
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