In:
Canadian Journal of Physiology and Pharmacology, Canadian Science Publishing, Vol. 93, No. 7 ( 2015-07), p. 527-534
Abstract:
This study was designed to investigate the influence of cytosolic Ca 2+ levels ([Ca 2+ ] i ) on action potential duration (APD) and on the incidence of early afterdepolarizations (EADs) in canine ventricular cardiomyocytes. Action potentials (AP) of isolated cells were recorded using conventional sharp microelectrodes, and the concomitant [Ca 2+ ] i was monitored with the fluorescent dye Fura-2. EADs were evoked at a 0.2 Hz pacing rate by inhibiting the rapid delayed rectifier K + current with dofetilide, by activating the late sodium current with veratridine, or by activating the L-type calcium current with BAY K8644. These interventions progressively prolonged the AP and resulted in initiation of EADs. Reducing [Ca 2+ ] i by application of the cell-permeant Ca 2+ chelator BAPTA-AM lengthened the AP at 1.0 Hz if it was applied alone, in the presence of veratridine, or in the presence of BAY K8644. However, BAPTA-AM shortened the AP if the cells were pretreated with dofetilide. The incidence of the evoked EADs was strongly reduced by BAPTA-AM in dofetilide, moderately reduced in veratridine, whereas EAD incidence was increased by BAPTA-AM in the presence of BAY K8644. Based on these experimental data, changes in [Ca 2+ ] i have marked effects on APD as well as on the incidence of EADs; however, the underlying mechanisms may be different, depending on the mechanism of EAD generation. As a consequence, reduction of [Ca 2+ ] i may eliminate EADs under some, but not all, experimental conditions.
Type of Medium:
Online Resource
ISSN:
0008-4212
,
1205-7541
DOI:
10.1139/cjpp-2014-0511
Language:
English
Publisher:
Canadian Science Publishing
Publication Date:
2015
detail.hit.zdb_id:
2004356-9
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