In:
Pharmacia, Pensoft Publishers, Vol. 69, No. 3 ( 2022-09-26), p. 883-890
Abstract:
In Vietnam, Physalis angulata L. has been widely used as a traditional medicine to treat fever, anti-inflammatory, and expectorant. Currently, there have been studies on the content of chemical composition especially physalin with anti-diabetic, anti-inflammatory, antibacterial, prevent cancer. This study developed a reliable and sensitive method to determine and validate simultaneous Physalin B and Physalin D in Physalis angulata L.. The QuEChERS method was used for sample preparation from leaf matrices and quantified by using High-performance liquid chromatography coupled with a diode-array detector. The method of research was validated under AOAC and ICH guidance. Chromatography conditions include Agilent C 18 column (250mm × 4,6mm; 5µm) with a gradient mode using acetonitrile – methanol-water as mobile phase. The recovery of the method ranged from 94.21 – 105.93% and RSD was from 1.20 – 2.31%, the LOD, and LOQ were 0.4 mg/kg – 2.4 mg/kg, respectively. The results of the study showed that the proposed the new QuEChERS method for quantification of Physalin B and D in Physalis angulata L. in Vietnam.
Type of Medium:
Online Resource
ISSN:
2603-557X
,
0428-0296
DOI:
10.3897/pharmacia.69.e89580
DOI:
10.3897/pharmacia.69.e89580.figure1
DOI:
10.3897/pharmacia.69.e89580.figure2
DOI:
10.3897/pharmacia.69.e89580.figure3
DOI:
10.3897/pharmacia.69.e89580.figure4
Language:
Unknown
Publisher:
Pensoft Publishers
Publication Date:
2022
detail.hit.zdb_id:
3010005-7
SSG:
15,3
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