In:
Journal of Clinical Microbiology, American Society for Microbiology, Vol. 45, No. 10 ( 2007-10), p. 3309-3315
Abstract:
Antibiograms and relevant genotypes of Korean avian pathogenic Escherichia coli (APEC) isolates ( n = 101) recovered between 1985 and 2005 were assessed via disc diffusion test, PCR, restriction enzyme analysis, and sequencing. These isolates were highly resistant to tetracycline (84.2%), streptomycin (84.2%), enrofloxacin (71.3%), and ampicillin (67.3%), and most of the tetracycline, streptomycin, enrofloxacin, and ampicillin resistances were associated with tetA and/or tetB , aadA and/or strA-strB , mutations in gyrA and/or parC , and TEM , respectively. Class 1 integrons were detected in 40 isolates (39.6%), and a variety of gene cassettes conferring streptomycin ( aadA ), gentamicin ( aadB ), and trimethoprim ( dfr ) resistances were identified: aadA1a (27.5%), dfrV - orfD (2.5%), aadB - aadA1a (2.5%), dfrI - aadA1a (47.5%), dfrXVII - aadA5 (12.5%), and dfrXII - orfF - aadA2 (7.5%). In addition, several types of common promoters (P ant ) of the gene cassettes (hybrid P1, weak P1, or weak P1 plus P2) and single-nucleotide polymorphisms in aadA1a were identified. The results of a chronological analysis demonstrated significant and continuous increases in the frequencies of resistances to several antibiotics (tetracycline, streptomycin, enrofloxacin, ampicillin, and trimethoprim-sulfamethoxazole) and of the relevant resistance genes ( tetA , strA-strB , and TEM ), mutations in gyrA and parC , and multidrug-resistant APEC strains during the period 2000 to 2005.
Type of Medium:
Online Resource
ISSN:
0095-1137
,
1098-660X
DOI:
10.1128/JCM.01922-06
Language:
English
Publisher:
American Society for Microbiology
Publication Date:
2007
detail.hit.zdb_id:
1498353-9
SSG:
12
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