In:
Angewandte Chemie, Wiley, Vol. 133, No. 36 ( 2021-09), p. 19919-19926
Abstract:
Systematic inactivation of nonribosomal peptide synthetase (NRPS) domains and translocation of the thioesterase (TE) domain revealed several unprecedented nonlinear NRPS assembly processes during the biosynthesis of the cyclodepsipeptide WS9326A in Streptomyces sp. SNM55. First, two sets of type ΙΙ TE (TEΙΙ)‐like enzymes mediate the shuttling of activated amino acids between two sets of stand‐alone adenylation (A)‐thiolation (T) didomain modules and an “A‐less” condensation (C)‐T module with distinctive specificities and flexibilities. This was confirmed by the elucidation of the affinities of the A‐T didomains for the TEΙΙs and its structure. Second, the C‐T didomain module operates iteratively and independently from other modules in the same protein to catalyze two chain elongation cycles. Third, this biosynthetic pathway includes the first example of module skipping, where the interpolated C and T domains are required for chain transfer.
Type of Medium:
Online Resource
ISSN:
0044-8249
,
1521-3757
DOI:
10.1002/ange.v133.36
DOI:
10.1002/ange.202103872
Language:
English
Publisher:
Wiley
Publication Date:
2021
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