In:
Proceedings, annual meeting, Electron Microscopy Society of America, Cambridge University Press (CUP), Vol. 48, No. 3 ( 1990-08-12), p. 438-439
Abstract:
This investigation has been carried out to observe different types of synaptic organizations in subesophageal ganglion of Pieris rapae , then to examine the ultrastructure of degenerated antennal receptor terminals in ipsilateral subesophageal ganglion following the removal of an left antenna, and finally to identify which type of synapse is constituted in the subesophageal ganglion by the axon terminal of the receptor cell projecting from the antenna. For electron microscopy of normal synaptic organizations in the subesophageal ganglion, the general anesthesia of the one-day-old cabbage butterflies was performed in keeping them at 4°C for about 30 min. Thereafter, the heads were cut off from the butterflies with a razor blade and prefixed in 1% paraformaldehyde-1% glutaraldehyde at 4°C for 1 h. After being prefixed for 1 h, the heads were dissected under the stereoscope for getting the intact brains. The brains continued to be kept in a new prefixative at 4°C overnight of completing the fixation. They were washed in 0.4M phosphate buffer, pH 7.4, containing 7% glucose and 0.5% CaCl 2 , and postfixed in 2% OsO 4 in phosphate buffer at 4°C for 2 h.
Type of Medium:
Online Resource
ISSN:
0424-8201
,
2690-1315
DOI:
10.1017/S0424820100159734
Language:
English
Publisher:
Cambridge University Press (CUP)
Publication Date:
1990
SSG:
11
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