In:
Hepatology Research, Wiley, Vol. 45, No. 4 ( 2015-04), p. 448-457
Abstract:
Livin, a member of the inhibitors of apoptosis proteins, is expressed in variable cancers, and its expression is considered a poor prognostic marker. The aims of this study were to observe the effect of L ivin on the behaviors of hepatocellular carcinoma ( HCC ) cells and to evaluate its expression in HCC tissues and its relation to prognosis. Methods The biological effects of L ivin on tumor cell behavior were investigated using siRNA in HepG2 and C hang cells. Migration, invasion and proliferation assays were performed. Flow cytometric analyses and western blotting were used to evaluate the impact of L ivin on apoptosis and the cell cycle. In addition, western blotting and immunohistochemistry were used to investigate L ivin expression in HCC tissues. Results L ivin knockdown suppressed tumor cell migration, invasion and proliferation in HCC cells, and increased the proportion of apoptotic cells as compared with scrambled siRNA ‐transfected HCC cells. Furthermore, L ivin knockdown resulted in the activation of caspases and increased apoptosis. In addition, L ivin knockdown modulated cell cycle regulatory protein levels such as decrease of cyclins and cyclin‐dependent kinase ( CDK ) level, and increase of CDK inhibitor ( CDKI ) level in HCC cells. The L ivin protein level was significantly elevated in HCC tissues as compared with normal hepatic tissues. However, L ivin expression was not found to be associated with clinicopathological parameters, which included patient survival. Conclusion These results suggest that L ivin is associated with invasive and oncogenic phenotypes of human HCC cells.
Type of Medium:
Online Resource
ISSN:
1386-6346
,
1872-034X
Language:
English
Publisher:
Wiley
Publication Date:
2015
detail.hit.zdb_id:
2006439-1
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