In:
Cells Tissues Organs, S. Karger AG, Vol. 206, No. 1-2 ( 2018), p. 95-105
Abstract:
〈 b 〉 〈 i 〉 Background: 〈 /i 〉 〈 /b 〉 Osteoarthritis (OA) is a degenerative joint disease, characterized by cartilage degradation and inflammation. The proinflammatory cytokine, interleukin (IL)-1β, plays a crucial role in the pathogenesis of OA by inducing the release of other catabolic factors that contribute to cartilage degradation. 〈 i 〉 Trifolium pratense 〈 /i 〉 L. (red clover) has been used as a medicinal plant in many countries and as a source of nutraceuticals to alleviate the symptoms of menopause. 〈 b 〉 〈 i 〉 Objectives: 〈 /i 〉 〈 /b 〉 In this study, we aimed to evaluate the anticatabolic effect of 40% prethanol extract of 〈 i 〉 T. pratense 〈 /i 〉 (40% PeTP) on IL-1β-stimulated chondrocytes. 〈 b 〉 〈 i 〉 Methods: 〈 /i 〉 〈 /b 〉 Primary rat chondrocytes were pretreated with 40% PeTP for 1 h before stimulation with IL-1β (20 ng/mL). The production of nitrite, prostaglandin E 〈 sub 〉 2 〈 /sub 〉 (PGE 〈 sub 〉 2 〈 /sub 〉 ), and aggrecan was measured by using Griess reagent and ELISA. Protein expression of inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2, matrix metalloproteinase (MMP)-1, MMP-3, MMP-13, A disintegrin and metalloproteinase with thrombospondin motif (ADAMTS)-4, mitogen-activated protein kinase (MAPK), and the nuclear factor (NF)-κB p65 subunit was measured by using Western blotting. 〈 b 〉 〈 i 〉 Results: 〈 /i 〉 〈 /b 〉 PeTP (40%) significantly inhibited the IL-1β-induced expression of nitrite, iNOS, PGE 〈 sub 〉 2 〈 /sub 〉 , COX-2, MMP-1, MMP-3, MMP-13, and ADAMTS-4 in isolated primary rat chondrocytes. Furthermore, 40% PeTP decreased the IL-1β-induced degradation of aggrecan, the phosphorylation of MAPKs, and the nuclear translocation of the NF-κB p65 subunit. 〈 b 〉 〈 i 〉 Conclusion: 〈 /i 〉 〈 /b 〉 These results suggested that 40% PeTP has a chondroprotective effect on inflammation and may be a potential preventative agent for OA progression.
Type of Medium:
Online Resource
ISSN:
1422-6405
,
1422-6421
Language:
English
Publisher:
S. Karger AG
Publication Date:
2018
detail.hit.zdb_id:
1481840-1
SSG:
12
Permalink