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  • 1
    In: Journal of Mass Spectrometry, Wiley, Vol. 40, No. 2 ( 2005-02), p. 214-226
    Abstract: Recent work on protein and peptide biomarker patterns revealed the difficulties in identifying their molecular components, which is indispensable for validation of the biological context. Cerebrospinal fluid and brain tissue are used as sources to discover new biomarkers, e.g. for neurodegenerative diseases. Many of these biomarker candidates are peptides with a molecular mass of 〈 10 kDa. Their identification is favourably achieved with a ‘top‐down’ approach, because this requires less purification and an enzymatic cleavage will often not yield enough specific fragments for successful database searches. Here, we describe an approach using quadrupole time‐of‐flight mass spectrometry (TOFMS) as a highly efficient mass spectrometric purification and identification tool after off‐line decomplexation of biological samples by liquid chromatography. After initial peptidomic screening with matrix‐assisted laser desorption/ionization (MALDI) TOFMS, the elution behaviour in chromatography and the exact molecular mass were used to locate the same signals in nanoelectrospray measurements. Most of the peaks detected in MALDI‐TOFMS could be retrieved in nanoelectrospray quadrupole TOFMS. Suitable collision energies for informative fragment spectra were investigated for different parent ions, charge states and molecular masses. After collision‐induced dissociation, the resulting fragmentation data of multiply charged ions can become much more complicated than those derived from tryptic peptide digests. However, the mass accuracy and resolution of quadrupole TOF instruments results in high‐quality data suitable for determining peptide sequences. The protein precursor, proteolytic processing and post‐translational modifications were identified by automated database searches. This is demonstrated by the exemplary identifications of thymosin beta‐4 (5.0 kDa) and NPY (4.3 kDa) from rat hypothalamic tissue and ubiquitin (8.6 kDa) from human cerebrospinal fluid. The high data quality should also allow for de novo identification. This methodology is generally applicable for peptides up to a molecular mass of about 10 kDa from body fluids, tissues or other biological sources. Copyright © 2005 John Wiley & Sons, Ltd.
    Type of Medium: Online Resource
    ISSN: 1076-5174 , 1096-9888
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2005
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  • 2
    In: Journal of Sports Sciences, Informa UK Limited, Vol. 38, No. 2 ( 2020-01-17), p. 130-139
    Type of Medium: Online Resource
    ISSN: 0264-0414 , 1466-447X
    Language: English
    Publisher: Informa UK Limited
    Publication Date: 2020
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  • 3
    In: Rapid Communications in Mass Spectrometry, Wiley, Vol. 15, No. 17 ( 2001-09-15), p. 1586-1592
    Abstract: Peptides with biological functions often contain disulfide bridges connecting two cysteine residues. In an attempt to screen biological fluids for peptides containing cysteine residues, we have developed a sensitive and specific method to label cysteines selectively and detect the resulting molecular mass shift by differential mass spectrometry. First, reduction of disulfide bridges and carboxyamidomethylation of free thiols is adjusted to quantitatively achieve cysteine alkylation for complex peptide extracts. In a second step, matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOFMS) before and after chemical derivatization is performed, followed by differential analysis to determine shifted peaks; shifted peaks belong to cysteine‐containing peptides, other peaks remain unchanged. The number of cysteines can then be determined by the resulting molecular mass shift. Free, reduced cysteines are shifted by 57 u, two oxidized cysteines involved in disulfide bridges (cystine) result in a shift to higher mass per disulfide bridge of 116 u. Disulfide bridges connecting different amino acid chains like insulin break up during reduction. In this case, two peaks with lower molecular masses result from a single one in the unmodified sample. With this technique, we were able to identify cysteine‐containing peptides and short fragments of proteins present in human blood filtrate. Copyright © 2001 John Wiley & Sons, Ltd.
    Type of Medium: Online Resource
    ISSN: 0951-4198 , 1097-0231
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2001
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  • 4
    In: Atmospheric Measurement Techniques, Copernicus GmbH, Vol. 16, No. 5 ( 2023-03-17), p. 1443-1460
    Abstract: Abstract. A new global O3 data product retrieved from the Michelson Interferometer for Passive Atmospheric Sounding (MIPAS) spectra with the IMK–IAA MIPAS data processor has been released. These data are based on ESA version 8 recalibrated radiance spectra with improved temporal stability. Changes in the level-2 processing with respect to previous data versions include the following: (1) the retrievals use improved temperature data and thus suffer less from the propagation of related errors. (2) The background continuum is now considered up to 58 km. (3) A priori information is now used to constrain the retrieval of the radiance offset. (4) Water vapour is fitted jointly with ozone to minimize the impact of interfering water lines. (5) A more adequate regularization has been chosen. (6) Ozone absorption lines in the MIPAS A band (685–980 cm−1) are used almost exclusively because of inconsistencies in spectroscopic data in the MIPAS AB band (1010–1180 cm−1). Only at altitudes above 50 km, where A-band ozone lines do not provide sufficient information, are ozone lines in the MIPAS AB band used. (7) Temperature-adjusted climatologies of vibrational temperatures of O3 and CO2 are considered to account for non-local thermodynamic equilibrium radiation. Ozone errors are estimated to be less than 10 % in the altitude range 20–50 km. The error budget is dominated by the spectroscopic errors, followed by the uncertainty of the instrumental line shape function, the gain calibration error, and the spectral noise. The error contribution of interfering gases is almost negligible. The vertical resolution depends on altitude and atmospheric conditions. In 2002–2004 it varies between 2.5 km at the lowest altitudes and 6 km at 70 km, while in 2005–2012 it covers 2 to 5.5 km in the same altitude range. The horizontal smearing in terms of the full width at half maximum of the horizontal component of the two-dimensional averaging kernel matrix is smaller than, or approximately equal to, the distance between two subsequent limb scans at all altitudes. This implies that the horizontal resolution is sampling-limited or optimal, respectively. An additional data version is made available that is free of the formal a priori information and thus more user-friendly for certain applications. Version 8 ozone results show a better consistency between the two MIPAS measurement periods. They seem to be more realistic than preceding data versions in terms of long-term stability, as at least a part of the drift is corrected. Further, the representation of elevated stratopause situations is improved.
    Type of Medium: Online Resource
    ISSN: 1867-8548
    Language: English
    Publisher: Copernicus GmbH
    Publication Date: 2023
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  • 5
    Online Resource
    Online Resource
    Ovid Technologies (Wolters Kluwer Health) ; 2017
    In:  Journal of Strength and Conditioning Research Vol. 31, No. 3 ( 2017-03), p. 608-619
    In: Journal of Strength and Conditioning Research, Ovid Technologies (Wolters Kluwer Health), Vol. 31, No. 3 ( 2017-03), p. 608-619
    Abstract: Julian, R, Meyer, T, Fullagar, HHK, Skorski, S, Pfeiffer, M, Kellmann, M, Ferrauti, A, and Hecksteden, A. Individual patterns in blood-borne indicators of fatigue—trait or chance. J Strength Cond Res 31(3): 608–619, 2017—Blood-borne markers of fatigue such as creatine kinase (CK) and urea (U) are widely used to fine-tune training recommendations. However, predictive accuracy is low. A possible explanation for this dissatisfactory characteristic is the propensity of athletes to react to different patterns of fatigue indicators (e.g., predominantly muscular [CK] or metabolic [U] ). The aim of the present trial was to explore this hypothesis by using repetitive fatigue-recovery cycles. A total of 22 elite junior swimmers and triathletes (18 ± 3 years) were monitored for 9 weeks throughout 2 training phases (low-intensity, high-volume [LIHV] and high-intensity, low-volume [HILV] phases). Blood samples were collected each Monday (recovered) and Friday (fatigued) morning. From measured values of CK, U, free-testosterone (FT), and cortisol (C) as determined in the rested and fatigued state, respectively, Monday–Friday differences (Δ) were calculated and classified by magnitude before calculation of ratios (ΔCK/ΔU and ΔFT/ΔC). Coefficient of variation (CV) was calculated as group-based estimate s of reproducibility. Linear mixed modeling was used to differentiate inter- and intraindividual variability. Consistency of patterns was analyzed by comparing with threshold values ( 〈 0.9 or 〉 1.1 for all weeks). Reproducibility was very low for fatigue-induced changes (CV ≥ 100%) with interindividual variation accounting for 45–60% of overall variability. Case-wise analysis indicated consistent ΔCK/ΔU patterns for 7 individuals in LIHV and 7 in HILV; 5 responded consistently throughout. For ΔFT/ΔC the number of consistent patterns was 2 in LIHV and 3 in HILV. These findings highlight the potential value of an individualized and multivariate approach in the assessment of fatigue.
    Type of Medium: Online Resource
    ISSN: 1064-8011
    Language: English
    Publisher: Ovid Technologies (Wolters Kluwer Health)
    Publication Date: 2017
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  • 6
    Online Resource
    Online Resource
    Ovid Technologies (Wolters Kluwer Health) ; 2016
    In:  Journal of Strength and Conditioning Research Vol. 30, No. 12 ( 2016-12), p. 3412-3427
    In: Journal of Strength and Conditioning Research, Ovid Technologies (Wolters Kluwer Health), Vol. 30, No. 12 ( 2016-12), p. 3412-3427
    Abstract: Raeder, C, Wiewelhove, T, Simola, RÁDP, Kellmann, M, Meyer, T, Pfeiffer, M, and Ferrauti, A. Assessment of fatigue and recovery in male and female athletes after 6 days of intensified strength training. J Strength Cond Res 30(12): 3412–3427, 2016—This study aimed to analyze changes of neuromuscular, physiological, and perceptual markers for routine assessment of fatigue and recovery in high-resistance strength training. Fourteen male and 9 female athletes participated in a 6-day intensified strength training microcycle (STM) designed to purposefully overreach. Maximal dynamic strength (estimated 1 repetition maximum [1RM est ]; criterion measure of fatigue and recovery); maximal voluntary isometric strength (MVIC); countermovement jump (CMJ) height; multiple rebound jump (MRJ) height; jump efficiency (reactive strength index, RSI); muscle contractile properties using tensiomyography including muscle displacement (Dm), delay time (Td), contraction time (Tc), and contraction velocity (V90); serum concentration of creatine kinase (CK); perceived muscle soreness (delayed-onset muscle soreness, DOMS) and perceived recovery (physical performance capability, PPC); and stress (MS) were measured before and after the STM and after 3 days of recovery. After completing the STM, there were significant ( p ≤ 0.05) performance decreases in 1RM est (%∆ ± 90% confidence limits, ES = effect size; −7.5 ± 3.5, ES = −0.21), MVIC (−8.2 ± 4.9, ES = −0.24), CMJ (−6.4 ± 2.1, ES = −0.34), MRJ (−10.5 ± 3.3, ES = −0.66), and RSI (−11.2 ± 3.8, ES = −0.73), as well as significantly reduced muscle contractile properties (Dm, −14.5 ± 5.3, ES = −0.60; V90, −15.5 ± 4.9, ES = −0.62). After days of recovery, a significant return to baseline values could be observed in 1RM est (4.3 ± 2.8, ES = 0.12), CMJ (5.2 ± 2.2, ES = 0.28), and MRJ (4.9 ± 3.8, ES = 0.32), whereas RSI (−7.9 ± 4.5, ES = −0.50), Dm (−14.7 ± 4.8, ES = −0.61), and V90 (−15.3 ± 4.7, ES = −0.66) remained significantly reduced. The STM also induced significant changes of large practical relevance in CK, DOMS, PPC, and MS before to after training and after the recovery period. The markers Td and Tc remained unaffected throughout the STM. Moreover, the accuracy of selected markers for assessment of fatigue and recovery in relation to 1RM est derived from a contingency table was inadequate. Correlational analyses also revealed no significant relationships between changes in 1RM est and all analyzed markers. In conclusion, mean changes of performance markers and CK, DOMS, PPC, and MS may be attributed to STM-induced fatigue and subsequent recovery. However, given the insufficient accuracy of markers for differentiation between fatigue and recovery, their potential applicability needs to be confirmed at the individual level.
    Type of Medium: Online Resource
    ISSN: 1064-8011
    Language: English
    Publisher: Ovid Technologies (Wolters Kluwer Health)
    Publication Date: 2016
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  • 7
    Online Resource
    Online Resource
    Ovid Technologies (Wolters Kluwer Health) ; 2017
    In:  Journal of Strength and Conditioning Research Vol. 31, No. 6 ( 2017-06), p. 1569-1576
    In: Journal of Strength and Conditioning Research, Ovid Technologies (Wolters Kluwer Health), Vol. 31, No. 6 ( 2017-06), p. 1569-1576
    Abstract: Schimpchen, J, Wagner, M, Ferrauti, A, Kellmann, M, Pfeiffer, M, and Meyer, T. Can cold water immersion enhance recovery in elite Olympic weightlifters? An individualized perspective. J Strength Cond Res 31(6): 1569–1576, 2017—We investigated whether cold water immersion (CWI) after intensive training sessions can enhance recovery in elite Olympic weightlifters, taking into account each athlete's individual response pattern. The entire German male Olympic weightlifting national team participated in the study ( n = 7), ensuring collection of data from elite athletes only. Using a randomized cross-over design, the athletes went through 2 high-intensity training microcycles consisting of 5 training sessions that were either followed by a CWI or passive recovery. Barbell speed in a snatch pull movement, blood parameters, and subjective ratings of general fatigue and recovery were assessed throughout the study. Physical performance at 2 snatch pull intensities (85% one repetition maximum [1RM]: −0.15% vs. −0.22%, p = 0.94; 90% 1RM: −0.7% vs. +1.23%, p = 0.25) did not differ significantly (condition × time). Although questionnaires revealed a significant decline in the ratings of overall recovery ( p 〈 0.001) and a significantly higher rating of overall stress ( p = 0.03) over time, no significant differences between conditions ( p = 0.14; p = 0.98) could be revealed. Similarly, neither of the analyzed blood parameters changed significantly between conditions over time (creatine kinase: p = 0.53; urea: p = 0.43; cortisol: p = 0.59; testosterone: p = 0.53; testosterone:cortisol ratio: p = 0.69). In general, CWI did not prove to be an effective tool to enhance recovery in elite Olympic weightlifters over a 3-day intensive training period. However, even though the group was rather homogeneous with regard to performance, there were considerable intersubject differences in their response to CWI. It seems that athletes are best advised on a case-by-case basis.
    Type of Medium: Online Resource
    ISSN: 1064-8011
    Language: English
    Publisher: Ovid Technologies (Wolters Kluwer Health)
    Publication Date: 2017
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  • 8
    Online Resource
    Online Resource
    IOS Press ; 2017
    In:  Work Vol. 58, No. 2 ( 2017-10-20), p. 193-202
    In: Work, IOS Press, Vol. 58, No. 2 ( 2017-10-20), p. 193-202
    Type of Medium: Online Resource
    ISSN: 1051-9815 , 1875-9270
    Language: Unknown
    Publisher: IOS Press
    Publication Date: 2017
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  • 9
    Online Resource
    Online Resource
    Springer Science and Business Media LLC ; 2016
    In:  Sport Sciences for Health Vol. 12, No. 3 ( 2016-12), p. 297-306
    In: Sport Sciences for Health, Springer Science and Business Media LLC, Vol. 12, No. 3 ( 2016-12), p. 297-306
    Type of Medium: Online Resource
    ISSN: 1824-7490 , 1825-1234
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2016
    detail.hit.zdb_id: 2181783-2
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  • 10
    Online Resource
    Online Resource
    Deutsche Zeitschrift Fur Sportmedizin/German Journal of Sports Medicine ; 2014
    In:  Deutsche Zeitschrift für Sportmedizin Vol. 2014, No. 05 ( 2014-05)
    In: Deutsche Zeitschrift für Sportmedizin, Deutsche Zeitschrift Fur Sportmedizin/German Journal of Sports Medicine, Vol. 2014, No. 05 ( 2014-05)
    Type of Medium: Online Resource
    ISSN: 0344-5925
    Uniform Title: Attitudes Towards Physical Activity and Exercise Participation – a Comparison of Healthy-Weight and Obese Adolescents
    RVK:
    Language: Unknown
    Publisher: Deutsche Zeitschrift Fur Sportmedizin/German Journal of Sports Medicine
    Publication Date: 2014
    detail.hit.zdb_id: 2018985-0
    detail.hit.zdb_id: 542455-0
    SSG: 31
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