In:
Journal of the American Society for Horticultural Science, American Society for Horticultural Science, Vol. 130, No. 6 ( 2005-11), p. 899-903
Abstract:
To produce the homozygous strain of a haploid plant derived from small seed-derived seedlings of `Banpeiyu' pummelo ( Citrus grandis Osbeck), we carried out colchicine treatment to axillary shoot buds of the haploid. Many shoots with cytochimeras (X+2X and 2X+4X) arose from the colchicine-treated axillary buds. When cytochimeric buds of 2X+4X were top-grafted onto trifoliate orange [ Poncirus trifoliata (L.) Raf.], a complete diploid shoot with 18 chromosomes was obtained from the cytochimera. This diploid strain showed vigorous growth compared with the original haploid. The leaf weight per unit area and the stomata size in this diploid were significantly larger than those of the original haploid plant, and were almost equal to those of `Banpeiyu' pummelo. The diploid strain was confirmed to be a doubled haploid of a haploid from `Banpeiyu' pummelo, based on random amplified polymorphic DNA (RAPD) analysis and chromosome composition analysis by chromomycin A 3 (CMA) staining.
Type of Medium:
Online Resource
ISSN:
0003-1062
,
2327-9788
DOI:
10.21273/JASHS.130.6.899
Language:
Unknown
Publisher:
American Society for Horticultural Science
Publication Date:
2005
detail.hit.zdb_id:
2040057-3
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