In:
PLOS Neglected Tropical Diseases, Public Library of Science (PLoS), Vol. 16, No. 6 ( 2022-6-6), p. e0009876-
Abstract:
Leptospirosis is among the most important zoonotic diseases in (sub-)tropical countries. The research objective was to evaluate the accuracy of the Serion IgM ELISA EST125M against the Microscopic Agglutination Test (MAT = imperfect reference test); to assess its ability to diagnose acute leptospirosis infections and to detect previous exposure to leptospires in an endemic setting. In addition, to estimate the overall Leptospira spp. seroprevalence in the Wiwa indigenous population in North-East Colombia. We analysed serum samples from confirmed leptospirosis patients from the Netherlands (N = 14), blood donor sera from Switzerland (N = 20), and sera from a cross-sectional study in Colombia (N = 321). All leptospirosis ELISA-positive, and a random of negative samples from Colombia were tested by the MAT for confirmation. The ELISA performed with a sensitivity of 100% (95% CI 77% - 100%) and a specificity of 100% (95% CI 83% - 100%) based on MAT confirmed Leptospira spp. positive and negative samples. In the cross-sectional study in Colombia, the ELISA performed with a sensitivity of 100% (95% CI 2–100%) and a specificity of 21% (95% CI 15–28%). Assuming a 5% Leptospira spp. seroprevalence in this population, the positive predictive value was 6% and the negative predictive value 100%. The Leptospira spp. seroprevalence in the Wiwas tested by the ELISA was 39%; however, by MAT only 0.3%. The ELISA is suitable to diagnose leptospirosis in acutely ill patients in Europe several days after onset of disease. For cross-sectional studies it is not recommended due to its low specificity. Despite the evidence of a high leptospirosis prevalence in other study areas and populations in Colombia, the Wiwa do not seem to be highly exposed to Leptospira spp.. Nevertheless, leptospirosis should be considered and tested in patients presenting with febrile illness.
Type of Medium:
Online Resource
ISSN:
1935-2735
DOI:
10.1371/journal.pntd.0009876
DOI:
10.1371/journal.pntd.0009876.g001
DOI:
10.1371/journal.pntd.0009876.t001
DOI:
10.1371/journal.pntd.0009876.t002
DOI:
10.1371/journal.pntd.0009876.t003
DOI:
10.1371/journal.pntd.0009876.t004
DOI:
10.1371/journal.pntd.0009876.t005
DOI:
10.1371/journal.pntd.0009876.s001
DOI:
10.1371/journal.pntd.0009876.s002
DOI:
10.1371/journal.pntd.0009876.s003
DOI:
10.1371/journal.pntd.0009876.r001
DOI:
10.1371/journal.pntd.0009876.r002
DOI:
10.1371/journal.pntd.0009876.r003
DOI:
10.1371/journal.pntd.0009876.r004
DOI:
10.1371/journal.pntd.0009876.r005
DOI:
10.1371/journal.pntd.0009876.r006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2429704-5
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