In:
HortScience, American Society for Horticultural Science, Vol. 40, No. 4 ( 2005-07), p. 1020D-1020
Abstract:
Pear scab caused by Venturianashicola is one of the most important diseases of oriental pear. Breeding a variety resistant to scab can be improved through marker-assisted selection (MAS). Bulked segregant analysis (BSA) and amplified fragment length polymorphic (AFLP) analysis were performed to identify DNA markers linked to the scab-resistant gene ( Vn ) using a population from a cross between PS2-93-3-98 (resistant parent) and Yali (susceptible parent). A total of 480 Eco R I/ Mse I primer combinations were used to identify markers specific to PS2-93-3-98 and resistant pool. Three AFLP markers linked to Vn , E-AGT/M-CCA 245 , E-ATT/M-CCG 300 , and E-GGT/M-TCT 225 , were selected. Linkage analysis between the selected markers and Vn locus was conducted with 51 individual plants. The selected markers, E-AGT/M-CCA 245 , E-ATT/M-CCG 300 , and E-GGT/M-TCT 225 , were located at 3.9, 3.8, and 1.2 cm away from Vn , respectively. For practical application, we are currently converting selected markers to simple PCR-based markers. The markers could be used to increase selection efficiency in pear-breeding programs for scab resistance.
Type of Medium:
Online Resource
ISSN:
0018-5345
,
2327-9834
DOI:
10.21273/HORTSCI.40.4.1020D
Language:
Unknown
Publisher:
American Society for Horticultural Science
Publication Date:
2005
detail.hit.zdb_id:
2040198-X
Permalink