In:
Current Neuropharmacology, Bentham Science Publishers Ltd., Vol. 21, No. 12 ( 2023-12), p. 2550-2562
Abstract:
OL-FS13, a neuroprotective peptide derived from Odorrana livida, can alleviate
cerebral ischemia-reperfusion (CI/R) injury, although the specific underlying mechanism remains to be further explored. Objective: The effect of miR-21-3p on the neural-protective effects of OL-FS13 was examined. Methods: In this study, the multiple genome sequencing analysis, double luciferase experiment,
RT-qPCR, and Western blotting were used to explore the mechanism of OL-FS13. Results: Showed that over-expression of miR-21-3p against the protective effects of OL-FS13 on oxygen-
glucose deprivation/re-oxygenation (OGD/R)-damaged pheochromocytoma (PC12) cells and in CI/R-injured rats. miR-21-3p was then found to target calcium/calmodulin-dependent protein kinase 2
(C AMKK2), and its overexpression inhibited the expression of CAMKK2 and phosphorylation of its
downstream adenosine 5’-monophosphate (AMP)-activated protein kinase (AMPK), thereby inhibiting the therapeutic effects of OL-FS13 on OGD/R and CI/R. Inhibition of CAMKK2 also antagonized
up-regulated of nuclear factor erythroid 2-related factor 2 (Nrf-2) by OL-FS13, thereby abolishing the antioxidant activity of the peptide. Conclusion: Our results showed that OL-FS13 alleviated OGD/R and CI/R by inhibiting miR-21-3p
to activate the CAMKK2/AMPK/Nrf-2 axis.
Type of Medium:
Online Resource
ISSN:
1570-159X
DOI:
10.2174/1570159X21666230502111013
Language:
English
Publisher:
Bentham Science Publishers Ltd.
Publication Date:
2023
detail.hit.zdb_id:
2119376-9
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