In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 71, No. 9 ( 2021-09-20)
Abstract:
An aerobic, Gram-stain-positive, non-motile, dull-yellow, short rod-shaped actinomycete strain, designated G10 T , was isolated from Tagetes patula (marigold) roots collected from Goyang in the Republic of Korea. The isolate showed best growth on Reasoner's 2A agar at 25 °C, pH 6.5.0 and with 0% NaCl (w/v). The strain was negative for oxidase activity and positive for catalase activity. On the basis of 16S RNA gene sequence similarity, strain G10 T was affiliated to the genus Nocardioides and the closest species were Nocardioides glacieisoli HLT3-15 T (98.8 %), Nocardioides zhouii HLT2-9 T (98.8 %), Nocardioides ganghwensis JC2055 T (98.7 %), Nocardioides cavernae YIM A1136 T (98.6 %), Nocardioides flavus Y4 T (98.5 %), Nocardioides oleivorans DSM 16090 T (98.3 %), Nocardioides alpinus Cr7-14 T (98.2 %), Nocardioides exalbidus DSM 22017 T (98.1 %) and Nocardioides hwasunensis KCTC 19197 T (98.1 %). Strain G10 T formed a monophyletic cluster with N. glacieisoli HLT3-15 T , N. zhouii HLT2-9 T and N. hwasunensis KCTC 19197 T in all phylogenetic trees. The cell-wall peptidoglycan of strain G10 T contained ll -diaminopimelic acid as the diagnostic amino acid. The predominant fatty acids were iso-C 16 : 0 and C 17 : 1 ω 8 c . MK-8(H 4 ) was the major isoprenoid quinone. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. Average nucleotide identity and digital DNA–DNA hybridization values were 78.6–88.7 % and 21.5–36.2 %, respectively, with the type strains of related species of the genus Nocardioides , suggesting that strain G10 T represents a novel species. The genome of strain G10 T is 4 231 000 bp long with a DNA G+C content of 71.5 mol% and encodes 4071 predicted proteins, six rRNAs and 46 tRNAs. The genome of strain G10 T comprises the biosynthetic gene cluster for T3PKS, terpene, NRPS-like fragment and RRE-containing element as secondary metabolites. The results of taxonomic, phylogenetic, biochemical, chemotaxonomic and genomic analysis clearly supported that strain G10 T represent a novel species within the genus Nocardioides , for which the name Nocardioides baculatus sp. nov is proposed and the type strain is G10 T (=KCTC 49626 T =NBRC 114801 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijsem.0.005012
Language:
English
Publisher:
Microbiology Society
Publication Date:
2021
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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