In:
Journal of Immunology Research, Hindawi Limited, Vol. 2020 ( 2020-07-30), p. 1-12
Abstract:
Celastrus orbiculatus Thunb has been known as an ethnopharmacological medicinal plant for antitumor, anti-inflammatory, and analgesic effects. Although various pharmacological studies of C . orbiculatus extract has been reported, an anti-inflammatory mechanism study of their phytochemical constituents has not been fully elucidated. In this study, compounds 1 – 17 , including undescribed podocarpane-type trinorditerpenoid ( 3 ), were purified from C . orbiculatus and their chemical structure were determined by high-resolution electrospray ionization mass (HRESIMS) and nuclear magnetic resonance (NMR) spectroscopic data. To investigate the anti-inflammatory activity of compounds 1 – 17 , nitric oxide (NO) secretion was evaluated in LPS-treated murine macrophages, RAW264.7 cells. Among compounds 1 – 17 , deoxynimbidiol ( 1 ) and new trinorditerpenoid ( 3 ) showed the most potent inhibitory effects (IC 50 : 4.9 and 12.6 μ M, respectively) on lipopolysaccharide- (LPS-) stimulated NO releases as well as proinflammatory mediators, such as inducible nitric oxide (iNOS), cyclooxygenase- (COX-) 2, interleukin- (IL-) 1 β , IL-6, and tumor necrosis factor- (TNF-) α . Its inhibitory activity of proinflammatory mediators is contributed by suppressing the activation of nuclear transcription factor- (NF-) κ B and mitogen-activated protein kinase (MAPK) signaling cascades including p65, inhibition of NF- κ B (I κ B), extracellular signal-regulated kinase (ERK), c-Jun NH 2 -terminal kinase (JNK), and p38. Therefore, these results demonstrated that diterpenoids 1 and 3 obtained from C . orbiculatus may be considered a potential candidate for the treatment of inflammatory diseases.
Type of Medium:
Online Resource
ISSN:
2314-8861
,
2314-7156
DOI:
10.1155/2020/7207354
Language:
English
Publisher:
Hindawi Limited
Publication Date:
2020
detail.hit.zdb_id:
2817541-4
Permalink