In:
Transplant Infectious Disease, Wiley, Vol. 16, No. 6 ( 2014-12), p. 930-940
Abstract:
Cytomegalovirus ( CMV )‐specific CD 8 + cytotoxic T lymphocytes ( CMV ‐ CTL s) play a crucial role in preventing CMV disease. However, the actual in vivo dynamics of CMV ‐ CTL clones after allogeneic hematopoietic stem cell transplantation (allo HCT ) are still unclear. Methods Using a single‐cell T‐cell receptor repertoire analysis, we monitored clones and chimerism of CMV ‐ CTL s in 3 CMV ‐seropositive allo HCT recipients from CMV ‐seronegative donors, with or without CMV reactivation. Results Nearly all of the CMV ‐ CTL s during follow‐up were CD 45 RA − CCR 7 − effector memory/ CD 45 RA + CCR 7 − effector T cells, and were highly matured. In each case, the use of BV gene families was restricted, especially in BV 5, 7, 28, and 29. Although no common predominant CMV ‐ CTL clones were found, several shared motifs of complementarity‐determining region‐3 were identified among the 3 cases; QGA in all, TGE and TDT in Case 1 and Case 2, and RDRG in Case 2 and Case 3. In all cases, CMV ‐ CTL clones that were detected for the first time after allo HCT persisted as the dominant clones. In Case 1, without CMV reactivation, recipient‐derived CMV ‐ CTL s exclusively persisted as a dominant clone, while all CMV ‐ CTL s in the other 2 cases, with CMV reactivation, were donor derived. Conclusion Clone monitoring and chimerism analyses should help to further clarify novel aspects of immuno‐reconstitution after allo HCT .
Type of Medium:
Online Resource
ISSN:
1398-2273
,
1399-3062
DOI:
10.1111/tid.2014.16.issue-6
Language:
English
Publisher:
Wiley
Publication Date:
2014
detail.hit.zdb_id:
2010983-0
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