In:
Journal of Neurochemistry, Wiley, Vol. 61, No. 3 ( 1993-09), p. 1050-1056
Abstract:
Abstract: Serotonin 5‐HT 2 receptor‐mediated intracellular Ca 2+ mobilization was investigated in rat glioma C6BU‐1 cells. The receptors became desensitized after previous exposure to 5‐HT in a time‐and concentration‐dependent manner. The desensitization of 5‐HT 2 receptor‐mediated intracellular signaling appeared to be homologous because previous exposure to 5‐HT did not alter the response to other transmitters such as thrombin or isoproterenol and because previous exposure to thrombin or isoproterenol did not diminish the response to 5‐HT. The desensitization induced by pretreatment with 5‐HT was potently prevented by the naphthalenesulfonamide derivative W‐7, a calmodulin antagonist, when it was cosupplied with 5‐HT. Furthermore, the preventive effect of W‐7 was greater than that of W‐5, a weak analogue of W‐7, and than that of H‐7, a nonselective inhibitor of protein kinases. These results suggest that 5‐HT 2 receptor‐mediated Ca 2+ mobilization can be desensitized homologously after prolonged exposure to 5‐HT in a calmodulin‐dependent manner in rat glioma C6BU‐1 cells.
Type of Medium:
Online Resource
ISSN:
0022-3042
,
1471-4159
DOI:
10.1111/jnc.1993.61.issue-3
DOI:
10.1111/j.1471-4159.1993.tb03619.x
Language:
English
Publisher:
Wiley
Publication Date:
1993
detail.hit.zdb_id:
2020528-4
SSG:
12
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