In:
Bioscience, Biotechnology, and Biochemistry, Informa UK Limited, Vol. 84, No. 4 ( 2020-04-02), p. 670-677
Kurzfassung:
Rapid DNA template preparation directly from a single rice (Oryza sativa) grain or rice flour of its equivalent weight was developed for loop-mediated isothermal amplification (LAMP). LAMP efficiency using DNA extract obtained from consecutive addition of alkaline lysis reagent (25 mM NaOH, 0.2 mM EDTA) and neutralizing reagent (40 mM Tris–HCl [pH 5]) was comparable to that using an equivalent amount of purified DNA as template. The stability of the prepared DNA extract was confirmed for up to six-day storage at room temperature. Without using any special laboratory devices, the developed method enabled a rapid, simple, and low-cost DNA template preparation method for reliable LAMP testing to detect rice genes.
Materialart:
Online-Ressource
ISSN:
0916-8451
,
1347-6947
DOI:
10.1080/09168451.2019.1701406
Sprache:
Englisch
Verlag:
Informa UK Limited
Publikationsdatum:
2020
ZDB Id:
2110940-0
SSG:
12
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