GLORIA

GEOMAR Library Ocean Research Information Access

X

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Online Resource
    Online Resource
    Molecular Detection of Neospora caninum from Naturally Infected Dogs in Van Province, East Turkey
    Agricultural Research Communication Center ; 2020
    In:  Indian Journal of Animal Research , No. of ( 2020-04-12)
    Details
    In: Indian Journal of Animal Research, Agricultural Research Communication Center, , No. of ( 2020-04-12)
    Abstract: Neospora caninum is a coccidian parasite causing abortion in cattle and neurological problems in horses. Dogs are definitive hosts of N. caninum. Polymerase chain reaction is the most specific method used for the detection of N. caninum oocytes. In the present study, a total of 100 fecal samples were collected from naturally infected dogs. Of the 100 samples analyzed, 11 of them were detected with Hammondia/Neospora-like oocytes. Genomic DNA was isolated using a commercially available DNA extraction kit. The Nc5 gene specific to N. caninum was amplified by PCR and two of the eleven samples with Hammondia/Neospora-like oocytes formed ~337 bp repeatable band. In conclusion, N. caninum, which has been shown to cause neurological disorders in dogs and to abortion in cattle, was detected in naturally infected dogs in Van Province in Polymerase chain reaction.
    Type of Medium: Online Resource
    ISSN: 0976-0555 , 0367-6722
    URL: Article
    DOI: 10.18805/ijar.B-1051
    Language: Unknown
    Publisher: Agricultural Research Communication Center
    Publication Date: 2020
    SSG: 22
    SSG: 12
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 2
    Online Resource
    Online Resource
    Molecular Survey of the Toxoplasma gondii and Neospora caninum in brain tissue of aborted fetuses of Morkaraman sheep in Muş, Türkiye
    Turkish Science and Technology Publishing (TURSTEP) ; 2023
    In:  Turkish Journal of Agriculture - Food Science and Technology Vol. 11, No. 12 ( 2023-12-28), p. 2407-2410
    Details
    In: Turkish Journal of Agriculture - Food Science and Technology, Turkish Science and Technology Publishing (TURSTEP), Vol. 11, No. 12 ( 2023-12-28), p. 2407-2410
    Abstract: Toxoplasma gondii and Neospora caninum are obligate intracellular protozoan parasites that can affect different warm-blooded species worldwide. In this study, it was aimed to detect T. gondii and N. caninum using PCR method in brain tissues of aborted sheep fetuses. Brain specimens were collected from 50 Morkaraman sheep fetuses that had undergone abortion at various stages of pregnancy, within the lambing seasons of 2023 in Muş. Approximately 1 cm³ of brain tissue from the right cerebral hemisphere was excised and subsequently frozen at -20°C for DNA extraction. DNA extraction and PCR amplification were then performed. As a result of this study, 11 (22%) of 50 brain tissues were positive. All brain samples examined in this study were negative for Neospora caninum. Based on the results of this study, it is possible to say that T. gondii is an important abortion agent in sheep in this region. Although N. caninum was not detected in this study, larger scale studies are recommended. Moreover, this study provides important information to breeders and veterinarians in the field in the evaluation and management of abortion.
    Type of Medium: Online Resource
    ISSN: 2148-127X
    URL: Article
    DOI: 10.24925/turjaf.v11i12.2407-2410.6480
    Language: Unknown
    Publisher: Turkish Science and Technology Publishing (TURSTEP)
    Publication Date: 2023
    detail.hit.zdb_id: 2764733-X
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 3
    Online Resource
    Online Resource
    A Molecular survey of Hepatozoon canis in dogs in the Siirt province of Turkey
    University of Veterinary Sciences Brno ; 2022
    In:  Acta Veterinaria Brno Vol. 91, No. 3 ( 2022), p. 277-283
    Details
    In: Acta Veterinaria Brno, University of Veterinary Sciences Brno, Vol. 91, No. 3 ( 2022), p. 277-283
    Abstract: This study aimed to determine Hepatozoon canis prevalence in dogs in the Siirt province of Turkey by the molecular method. The animal material of the study consisted of a total of 75 dogs that appeared clinically healthy. Two ml of blood sample were taken from the vena cephalica antebrachii. Then, DNA extraction was performed. Polymerase chain reaction (PCR) was performed to amplify the 666 bp 18S rRNA gene region of Hepatozoon canis . Two positive PCR products were purified and sequenced. As a result of Nested-PCR, H. canis specific bands in 666 bp size were obtained in 7 (9.33%) out of 75 dogs. The result of sequence analysis, the nucleotide sequence was registered in the NCBI GenBank database with accession numbers OL467380.1-OL467538.1. Hepatozoon canis registered in GenBank of sequence OL467380.1 was found to be similar with other H. canis strains of registration numbers MW684292.1 with 99.69% and MH615006.1-MK091085.1-MF797806.1 with 99.53% rates; and the sequence with registration number OL467538.1 was found to be similar to the series MW684291.1 with 99.09% and MH615006.1-MK091085.1-KX 818220.1 with 99.08% rates by BLAST analysis. Hepatozoon canis prevalence of dogs in the Siirt province was determined as a result of this study. It is of great importance to take preventive measures, especially to fight ticks with appropriate acaricides, since there is no vaccine to prevent the disease.
    Type of Medium: Online Resource
    ISSN: 0001-7213 , 1801-7576
    URL: Article
    DOI: 10.2754/avb202291030277
    Language: English
    Publisher: University of Veterinary Sciences Brno
    Publication Date: 2022
    detail.hit.zdb_id: 2106644-9
    SSG: 22
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 4
    Online Resource
    Online Resource
    Molecular Identification of Ehrlichia canis in Rhipicephalus Sanguineus Ticks from Siirt Province
    Walter de Gruyter GmbH ; 2021
    In:  Acta Veterinaria Vol. 71, No. 3 ( 2021-09-01), p. 247-255
    Details
    In: Acta Veterinaria, Walter de Gruyter GmbH, Vol. 71, No. 3 ( 2021-09-01), p. 247-255
    Abstract: This study was performed on Ehrlichia canis positive ticks collected from dogs to perform sequencing of their 16S rRNA genetic section using the PCR method. The collection of ticks was performed from a total of 60 dogs in the Siirt province, Turkey. A total of 250 ticks were collected and morphologically investigated. All ticks were identified as Rhipicephalus sanguineus sensu lato (s.l) . Ehrlichial DNA was detected by the PCR method performed on 38 (15.2 %) of the ticks. The E. canis strains obtained as a result of the sequence analysis were found to be 100% identical to the American Texas (MH620194), Indian (KX766395), and Egyptian (MG564254) strains. This study thereby has identified a zoonotic agent from the R. sanguineus ticks collected from the dogs in the Siirt province.
    Type of Medium: Online Resource
    ISSN: 1820-7448
    URL: Article
    DOI: 10.2478/acve-2021-0022
    Language: English
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2021
    detail.hit.zdb_id: 2380825-1
    SSG: 22
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 5
    Online Resource
    Online Resource
    Preliminary investigation of the prevalence and genotype distribution of Cryptosporidium spp ., and Giardia duodenalis in cats in Siirt, Turkey
    Walter de Gruyter GmbH ; 2023
    In:  Acta Veterinaria Vol. 73, No. 3 ( 2023-09-01), p. 317-324
    Details
    In: Acta Veterinaria, Walter de Gruyter GmbH, Vol. 73, No. 3 ( 2023-09-01), p. 317-324
    Abstract: Cryptosporidium spp. and Giardia duodenalis are protozoan parasites found in humans and many animal species worldwide. The aim of this study was to determine the prevalence and genotypes of Cryptosporidium spp. and Giardia duodenalis in cats and to evaluate the zoonotic potential of these agents. The animal material of the study consisted of a total of 40 cats brought to the Veterinary Faculty. Fresh fecal samples taken from the cats were placed in individual sample containers. All samples were examined under the microscope by Kinyoun Acid Fast staining for Cryptosporidium spp. and by the native-Lugol method for Giardia duodenalis . Nested PCR and sequence analyses were then performed. As a result of microscopic and nested PCR analyses for Cryptosporidium spp., no positivity was found in any sample. The prevalence of Giardia duodenalis was 2.5% in both microscopic examination and nested PCR analyses. When the DNA sequences of the β-Giardin gene obtained in the study were compared with the database in NCBI Basic Local Alignment Search Tool, it was determined that one sample overlapped with Assemblage B samples. As a result of this study, the prevalence of Cryptosporidium spp. and Giardia duodenalis in cats was determined and the presence of Assemblage B was revealed. It is recommended that repetitive studies should be carried out as much as possible to determine the possible role of these parasites in the transmission of these parasites to humans.
    Type of Medium: Online Resource
    ISSN: 1820-7448
    URL: Article
    DOI: 10.2478/acve-2023-0024
    Language: English
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2023
    detail.hit.zdb_id: 2380825-1
    SSG: 22
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 6
    Online Resource
    Online Resource
    Investigation of Toxoplasma gondii and Neospora caninum in different tissues of aborted foetuses of sheep in Van Province, Türkiye: Analysis by nested PCR, histopathological and immunohistochemical methods
    University of Veterinary Sciences Brno ; 2023
    In:  Acta Veterinaria Brno Vol. 92, No. 2 ( 2023), p. 123-131
    Details
    In: Acta Veterinaria Brno, University of Veterinary Sciences Brno, Vol. 92, No. 2 ( 2023), p. 123-131
    Abstract: Toxoplasma gondii and Neospora caninum are protozoon parasites from the intracellular apicomplexan family. Toxoplasma gondii is the cause of health and economic problems in the sheep industry worldwide. Neospora caninum is usually reported in cows and leads to infections causing abortions; however, its prevalence in sheep is not clear. The present study aimed to investigate the prevalence and pathology of T. gondii and N. caninum by PCR, histopathological and immune-histochemical methods in aborted sheep foetuses collected at different sheep flocks in the Van Province, Türkiye, in 2021. Firstly, the DNA of T. gondii and N. caninum were investigated by PCR in the brain, heart, and peritoneal fluid samples from 42 sheep foetuses. Toxoplasma gondii DNA was proved in 35.7% (15/42) of foetuses whereas N. caninum DNA was not determined in any of the samples. Histopathologically, all T. gondii positive brain tissue samples showed lymphohistiocytic multifocal encephalomyelitis and additional findings included necrotizing myocarditis in the positive heart samples. Toxoplasma gondii tachyzoites were identified in the lesions (diffuse or focal mononuclear cell infiltration in the meninges, and microglia proliferation, myocarditis with oedema) by anti- T. gondii antibodies by the immunohistochemical method. Based on our results, we can conclude that T. gondii is an important agent in sheep abortions and the PCR method is a suitable method for diagnosis which can also be used in heart tissue in pathological studies.
    Type of Medium: Online Resource
    ISSN: 0001-7213 , 1801-7576
    URL: Article
    DOI: 10.2754/avb202392020123
    Language: English
    Publisher: University of Veterinary Sciences Brno
    Publication Date: 2023
    detail.hit.zdb_id: 2106644-9
    SSG: 22
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 7
    Online Resource
    Online Resource
    Prevalence and molecular characterization of Cryptosporidium spp. in calves in the Siirt Province, Türkiye
    University of Veterinary Sciences Brno ; 2023
    In:  Acta Veterinaria Brno Vol. 92, No. 3 ( 2023), p. 243-250
    Details
    In: Acta Veterinaria Brno, University of Veterinary Sciences Brno, Vol. 92, No. 3 ( 2023), p. 243-250
    Abstract: Cryptosporidiosis, one of the main protozoan infections of the last century, is especially dangerous for calves and causes significant economic losses. This research was carried out to determine the prevalence of Cryptosporidium spp. by microscopic and molecular methods and to determine subtypes in 100 calves up to 6 months old and with diarrhoea in the Siirt Province, Türkiye. As a result of the microscopic examination (Kinyoun’s acid-fast), Cryptosporidium spp. oocysts were found in 8 (8%) of 100 samples. As a result of nested PCR, 826-864 bp specific bands for Cryptosporidium spp. were obtained in 13 (13%) of 100 samples. When the DNA sequences of the SSU rRNA gene were compared with the NCBI Basic Local Alignment Search Tool database, it was determined that eight samples sequence analyses showed 100% similarity with the C. parvum , C. ryanae , and C. bovis samples. The detection of C. parvum , which has zoonotic importance in this study, suggests that calves with diarrhoea may be a source of contamination for other animals and humans. Therefore, animal owners and people in close contact with animals should be informed about the public health of cryptosporidiosis.
    Type of Medium: Online Resource
    ISSN: 0001-7213 , 1801-7576
    URL: Article
    DOI: 10.2754/avb202392030243
    Language: English
    Publisher: University of Veterinary Sciences Brno
    Publication Date: 2023
    detail.hit.zdb_id: 2106644-9
    SSG: 22
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 8
    Online Resource
    Online Resource
    The Investigation of Giardiasis (Foodborne and Waterborne Diseases) in Buffaloes in Van Region, Türkiye: First Molecular Report of Giardia duodenalis Assemblage B from Buffaloes
    MDPI AG ; 2023
    In:  Pathogens Vol. 12, No. 1 ( 2023-01-08), p. 106-
    Details
    In: Pathogens, MDPI AG, Vol. 12, No. 1 ( 2023-01-08), p. 106-
    Abstract: Giardia duodenalis (G. duodenalis) is an important zoonotic protozoan agent that causes foodborne and waterborne diarrhea in humans and other mammals. Molecular-based tests are critical in diagnosing giardiasis in humans and animals, identifying species, understanding the zoonotic potential and transmission routes, and evaluating taxonomy. Therefore, this study aimed to investigate the molecular characterization of G. duodenalis in buffaloes in the Van region in Türkiye. Buffaloes are a species that has been poorly studied in this regard. For this purpose, 100 fecal samples were collected from buffaloes in the Van region. The DNA extraction was performed using the GeneMATRIX STOOL DNA Purification Kit from stool samples. The nested PCR test was performed with the appropriate primers from the obtained DNA samples. The obtained bands suitable for sequencing were sent for sequence analysis, and the sequence results were aligned bidirectionally and compared with the database of GenBank by BLAST. As a result of the study, an 11% positivity rate for G. duodenalis was found in buffaloes, and assemblage E and assemblage B were isolated. To our knowledge, assemblage B in buffaloes was reported for the first time in this study. As a result, it was concluded that buffaloes are an important reservoir for waterborne and foodborne giardiasis.
    Type of Medium: Online Resource
    ISSN: 2076-0817
    URL: Article
    DOI: 10.3390/pathogens12010106
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2695572-6
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 9
    Online Resource
    Online Resource
    Determination of Cryptosporidium spp. in Van cats by nested PCR
    Medycyna Weterynaryjna - Redakcja ; 2018
    In:  Medycyna Weterynaryjna Vol. 74, No. 1 ( 2018), p. 6008-2018
    Details
    In: Medycyna Weterynaryjna, Medycyna Weterynaryjna - Redakcja, Vol. 74, No. 1 ( 2018), p. 6008-2018
    Abstract: The present study was conducted to investigate the presence of Cryptosporidium spp. agents in cats from the Turkish Van Cat Shelter at YüzüncüYıl University by a modified Ziehl-Neelsen staining method and nested PCR. Individual stool samples were obtained from 30 adult females, 30 adult males and 40 kittens – a total of 100 Van cats were analyzed in the study. A simplified formol-ether concentration method was applied to all samples. The samples were then examined microscopically by a modified Ziehl-Neelsen staining method. As a result of the staining, Cryptosporidium spp. oocysts were identified in stool samples of 3 kittens in the microscopic examination. After that, PCR and nested-PCR were conducted with suitable primers. Nested PCR identified 5 kittens (5%) as positive. As a result, it was concluded that nested PCR was a superior diagnostic method for Cryptosporidium diagnosis compared with staining methods and that infected cats could be a health hazard for other cats and individuals, since Cryptosporidium spp. agents infect via the faecal-oral route. Therefore, we believe it is necessary to raise the awareness of people in contact with cats..
    Type of Medium: Online Resource
    ISSN: 0025-8628
    URL: Article
    DOI: 10.21521/mw.6008
    Language: Unknown
    Publisher: Medycyna Weterynaryjna - Redakcja
    Publication Date: 2018
    detail.hit.zdb_id: 2777172-6
    SSG: 22
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 10
    Online Resource
    Online Resource
    MOLECULAR INVESTIGATION OF SOME BACTERIA (COXİELLA BURNETİİ, MYCOPLASMA HAEMOCANİS, CANDİDATUS MYCOPLASMA HAEMATOPARVUM, WOLBACHİA) IN RHIPICEPHALUS SANGUINEUS TICKS IN SIIRT PROVINCE, TURKEY
    Egypts Presidential Specialized Council for Education and Scientific Research ; 2022
    In:  Assiut Veterinary Medical Journal Vol. 0, No. 0 ( 2022-10-20), p. 28-38
    Details
    In: Assiut Veterinary Medical Journal, Egypts Presidential Specialized Council for Education and Scientific Research, Vol. 0, No. 0 ( 2022-10-20), p. 28-38
    Type of Medium: Online Resource
    ISSN: 2314-5226
    URL: Article
    DOI: 10.21608/avmj.2022.144467.1066
    Language: English
    Publisher: Egypts Presidential Specialized Council for Education and Scientific Research
    Publication Date: 2022
    detail.hit.zdb_id: 2929279-7
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...