In:
Türk Tarım ve Doğa Bilimleri Dergisi, Turk Tarim ve Doga Bilimleri Dergisi, Vol. 8, No. 1 ( 2021-01-23), p. 179-187
Abstract:
In this research, determination of phylogenetic tree of sheep in Şanlıurfa province using molecular techniques was the main goal. White Karaman (AK) and Awassi sheep (IV) breed raised in Şanlıurfa province were used as the animal materials. Fleece samples were collected for genomic DNA isolation in sheep, and genomic DNAs were isolated in all the samples. In DNA samples, necessary forward and reverse primers were designed to amplify mitochondrial Cytochrome b (Cyt b) gene region. Mitochondrial Cyt b gene region were amplified by applying polymerase chain reaction (PCR) technique, and gene sequence information of PCR products were obtained. The rate of G+C, number of polymorphic site (S), number of haplotypes (h), haplotype diversity (Hd), nucleotide diversity (π) and total number of region for all populations were calculated. N-J phylogenetic tree formed in this research using sheep haplotype Cyt b sequences and reference sequences ( for A, B, C, D and E lineage), 6 haplotypes (IV01, AK13, AK09, IV06, AK16, IV05) out of 16 haplotypes were in B lineage, 1 haplotype (IV12) were in A lineage, 1 haplotype (AK04) were in E lineage, 5 haplotypes (AK12, AK02, IV16, AK01, IV08, and IV03) were in C lineage, and AK06 and IV13 were in different group. In conclusion, in White Karaman and Awassi sheep, raised in Şanlıurfa province; gene sequences of Cytochrome b gene were determined.
Type of Medium:
Online Resource
ISSN:
2148-3647
DOI:
10.30910/turkjans.798488
Language:
Unknown
Publisher:
Turk Tarim ve Doga Bilimleri Dergisi
Publication Date:
2021
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