GLORIA

GEOMAR Library Ocean Research Information Access

X

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Online Resource
    Online Resource
    Assessment of method reliability and determination of reference intervals for rotational thromboelastometry in horses
    Wiley ; 2016
    In:  Journal of Veterinary Emergency and Critical Care Vol. 26, No. 5 ( 2016-09), p. 691-703
    Details
    In: Journal of Veterinary Emergency and Critical Care, Wiley, Vol. 26, No. 5 ( 2016-09), p. 691-703
    Abstract: To assess the measurement reliability of rotational thromboelastometry (ROTEM) measurements in horses, establish reference intervals for healthy horses, and evaluate the relationship between ROTEM variables, hematologic variables, and standard coagulation tests. Design Prospective observational study. Setting University teaching hospital. Animals Fifty healthy and 10 diseased adult horses. Interventions None. Measurements and Main Results Blood was sampled from 10 healthy and 10 diseased horses and samples were repeatedly analyzed to evaluate measurement reliability of various ROTEM variables. Four different ROTEM assays (ie, EXTEM, INTEM, FIBTEM, and APTEM) were run simultaneously under standardized conditions. The device‐related, operator‐related, and day‐to‐day variability for the majority of ROTEM variables was very low to low, as indicated by a coefficient of variation (CV) of 〈 15%. Most of test‐retest variability of ROTEM variables appeared to be device‐related. Blood samples from 50 clinically healthy horses were used to establish reference intervals for ROTEM variables. Multiple stepwise regression analyses identified associations of different ROTEM variables with hematocrit, total protein concentration, fibrinogen concentration, platelet count, prothrombin time, activated partial thromboplastin time, and thrombin time. Conclusions ROTEM is a feasible method to evaluate coagulation in horses. Its measurement reliability is acceptable, but device‐related measurement variability has to be considered. Reference intervals are presented, but the influence of hematocrit, platelet count, and fibrinogen concentration may need to be taken into account when interpreting individual test results.
    Type of Medium: Online Resource
    ISSN: 1479-3261 , 1476-4431
    URL: Issue
    URL: Article
    DOI: 10.1111/vec.2016.26.issue-5
    DOI: 10.1111/vec.12515
    Language: English
    Publisher: Wiley
    Publication Date: 2016
    detail.hit.zdb_id: 2077212-9
    SSG: 22
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 2
    Online Resource
    Online Resource
    An Equine Model for Vaccination against a Hepacivirus: Insights into Host Responses to E2 Recombinant Protein Vaccination and Subsequent Equine Hepacivirus Inoculation
    MDPI AG ; 2022
    In:  Viruses Vol. 14, No. 7 ( 2022-06-27), p. 1401-
    Details
    In: Viruses, MDPI AG, Vol. 14, No. 7 ( 2022-06-27), p. 1401-
    Abstract: Equine hepacivirus (EqHV) is the closest known genetic homologue of hepatitis C virus. An effective prophylactic vaccine is currently not available for either of these hepaciviruses. The equine as potential surrogate model for hepacivirus vaccine studies was investigated, while equine host responses following vaccination with EqHV E2 recombinant protein and subsequent EqHV inoculation were elucidated. Four ponies received prime and booster vaccinations (recombinant protein, adjuvant) four weeks apart (day −55 and −27). Two control ponies received adjuvant only. Ponies were inoculated with EqHV RNA-positive plasma on day 0. Blood samples and liver biopsies were collected over 26 weeks (day −70 to +112). Serum analyses included detection of EqHV RNA, isotypes of E2-specific immunoglobulin G (IgG), nonstructural protein 3-specific IgG, haematology, serum biochemistry, and metabolomics. Liver tissue analyses included EqHV RNA detection, RNA sequencing, histopathology, immunohistochemistry, and fluorescent in situ hybridization. Al-though vaccination did not result in complete protective immunity against experimental EqHV inoculation, the majority of vaccinated ponies cleared the serum EqHV RNA earlier than the control ponies. The majority of vaccinated ponies appeared to recover from the EqHV-associated liver insult earlier than the control ponies. The equine model shows promise as a surrogate model for future hepacivirus vaccine research.
    Type of Medium: Online Resource
    ISSN: 1999-4915
    URL: Article
    DOI: 10.3390/v14071401
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2516098-9
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 3
    Online Resource
    Online Resource
    Validation of the Accutrend Plus point‐of‐care triglyceride analyzer in horses, ponies, and donkeys
    Wiley ; 2016
    In:  Journal of Veterinary Emergency and Critical Care Vol. 26, No. 5 ( 2016-09), p. 682-690
    Details
    In: Journal of Veterinary Emergency and Critical Care, Wiley, Vol. 26, No. 5 ( 2016-09), p. 682-690
    Abstract: To assess the accuracy and reliability of a point‐of‐care (POC) triglyceride analyzer and to establish reference intervals for blood ([TRIG] POC/WB ) and plasma triglyceride concentrations ([TRIG] POC/PL ) in horses, ponies, and donkeys. Design Prospective study. Setting University teaching hospital. Animals 120 adult healthy equids (78 horses and ponies, 42 donkeys) and 79 equids suffering from hypertriglyceridemia (73 horses and ponies, 6 donkeys). Interventions None. Measurements and Main Results [TRIG] POC/WB and [TRIG] POC/PL were measured using a POC analyzer and plasma triglyceride concentrations were measured using a standard laboratory assay ([TRIG] LAB/PL ). Reference intervals were determined. Test accuracy was assessed by Bland–Altman comparison of POC measurements with standard laboratory measurements and by evaluating linearity of dilutional series. Test reliability was assessed by repeated serial measurements. [TRIG] POC/WB and [TRIG] POC/PL were below the analytic range of the POC assay ( 〈 0.8 mmol/L [ 〈 70 mg/dL]) in healthy horses and ponies, whereas the reference intervals were 0.82–3.14 mmol/L (73–278 mg/dL) and 0.87–3.02 mmol/L (77–267 mg/dL), respectively, in donkeys. The POC analyzer systematically overestimated triglyceride concentrations when compared to a standard laboratory assay. The difference between [TRIG] POC/WB and [TRIG] POC/PL was small and clinically negligible. The coefficient of variation of repeated measures performed on the POC analyzer was below 10% for [TRIG] POC/WB and [TRIG] POC/PL , both in horses and donkeys and at all concentration ranges. Conclusions The POC analyzer allows accurate and reliable measurement of [TRIG] POC/WB and [TRIG] POC/PL in horses, ponies, and donkeys in clinical settings. Assay‐specific reference intervals should be determined for diagnosis and clinical monitoring of hypertriglyceridemia in equids.
    Type of Medium: Online Resource
    ISSN: 1479-3261 , 1476-4431
    URL: Issue
    URL: Article
    DOI: 10.1111/vec.2016.26.issue-5
    DOI: 10.1111/vec.12512
    Language: English
    Publisher: Wiley
    Publication Date: 2016
    detail.hit.zdb_id: 2077212-9
    SSG: 22
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 4
    Online Resource
    Online Resource
    Prospective observational cohort study on grading the severity of postoperative complications in global surgery research
    Oxford University Press (OUP) ; 2019
    In:  British Journal of Surgery Vol. 106, No. 2 ( 2019-01-08), p. e73-e80
    Details
    In: British Journal of Surgery, Oxford University Press (OUP), Vol. 106, No. 2 ( 2019-01-08), p. e73-e80
    Abstract: The Clavien–Dindo classification is perhaps the most widely used approach for reporting postoperative complications in clinical trials. This system classifies complication severity by the treatment provided. However, it is unclear whether the Clavien–Dindo system can be used internationally in studies across differing healthcare systems in high- (HICs) and low- and middle-income countries (LMICs). Methods This was a secondary analysis of the International Surgical Outcomes Study (ISOS), a prospective observational cohort study of elective surgery in adults. Data collection occurred over a 7-day period. Severity of complications was graded using Clavien–Dindo and the simpler ISOS grading (mild, moderate or severe, based on guided investigator judgement). Severity grading was compared using the intraclass correlation coefficient (ICC). Data are presented as frequencies and ICC values (with 95 per cent c.i.). The analysis was stratified by income status of the country, comparing HICs with LMICs. Results A total of 44 814 patients were recruited from 474 hospitals in 27 countries (19 HICs and 8 LMICs). Some 7508 patients (16·8 per cent) experienced at least one postoperative complication, equivalent to 11 664 complications in total. Using the ISOS classification, 5504 of 11 664 complications (47·2 per cent) were graded as mild, 4244 (36·4 per cent) as moderate and 1916 (16·4 per cent) as severe. Using Clavien–Dindo, 6781 of 11 664 complications (58·1 per cent) were graded as I or II, 1740 (14·9 per cent) as III, 2408 (20·6 per cent) as IV and 735 (6·3 per cent) as V. Agreement between classification systems was poor overall (ICC 0·41, 95 per cent c.i. 0·20 to 0·55), and in LMICs (ICC 0·23, 0·05 to 0·38) and HICs (ICC 0·46, 0·25 to 0·59). Conclusion Caution is recommended when using a treatment approach to grade complications in global surgery studies, as this may introduce bias unintentionally.
    Type of Medium: Online Resource
    ISSN: 0007-1323 , 1365-2168
    URL: Article
    DOI: 10.1002/bjs.11025
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2019
    detail.hit.zdb_id: 2006309-X
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 5
    Online Resource
    Online Resource
    The surgical safety checklist and patient outcomes after surgery: a prospective observational cohort study, systematic review and meta-analysis
    Elsevier BV ; 2018
    In:  British Journal of Anaesthesia Vol. 120, No. 1 ( 2018-01), p. 146-155
    Details
    In: British Journal of Anaesthesia, Elsevier BV, Vol. 120, No. 1 ( 2018-01), p. 146-155
    Type of Medium: Online Resource
    ISSN: 0007-0912
    URL: Article
    DOI: 10.1016/j.bja.2017.08.002
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2018
    detail.hit.zdb_id: 2011968-9
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...