In:
Natural Product Communications, SAGE Publications, Vol. 18, No. 6 ( 2023-06)
Abstract:
In this study, an online CMC–LC/MS method was established in order to screen the active components of Yiqi Fumai lyophilization injection (YQFM) related to angiogenesis. The pro-angiogenic active components of YQFM were screened using this system and were then further verified. Methods The ECV304/CMC model coupled online with an HPLC–MS system was initially developed to screen, analyze, and identify the allergenic components of YQFM. A fraction was retained on the ECV304/CMC column, which was identified as ginsenoside Rb 2 . A cell proliferation assay and zebrafish intestinal microvascular model labeled with green fluorescence were used to detect the proliferation effect of ginsenoside Rb 2 . Results (1) The active component in YQFM, which was retained on the column, was ginsenoside Rb 2 . (2) Within a concentration range of 2.5–20 μg/mL, ginsenoside Rb 2 was shown to promote the proliferation of ECV304 and EPCs cells and increase the area of sub-intestinal vessels in zebrafish. Conclusion Ginsenoside Rb 2 , the active component in YQFM, can promote angiogenesis. The ECV304/CMC-online-HPLC system may serve as an effective screening tool for the vasoactive components from the traditional Chinese medicine injection.
Type of Medium:
Online Resource
ISSN:
1934-578X
,
1555-9475
DOI:
10.1177/1934578X231182209
Language:
English
Publisher:
SAGE Publications
Publication Date:
2023
detail.hit.zdb_id:
2430442-6
SSG:
15,3
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