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  • 1
    Online Resource
    Online Resource
    Diagrama causal del efecto biocontrolador de la bacteria Pasteuria sp. sobre el nematodo fitoparásito Hirschmanniella spp. en el cultivo de arroz (Oryza sativa L.)
    Centro de Investigacion y Desarrollo Profesional - CIDEPRO ; 2021
    In:  Pro Sciences: Revista de Producción, Ciencias e Investigación Vol. 5, No. 38 ( 2021-04-22), p. 70-85
    Details
    In: Pro Sciences: Revista de Producción, Ciencias e Investigación, Centro de Investigacion y Desarrollo Profesional - CIDEPRO, Vol. 5, No. 38 ( 2021-04-22), p. 70-85
    Abstract: La bacteria Pasteuria sp. actúa como agente biocontrolador de nematodos en diferentes cultivos. En la actualidad los rendimientos del cultivo del arroz podrán estar siendo afectados negativamente a nivel global y en el Ecuador por efecto del nematodo fitoparásito Hirschmanniella spp. El objetivo de esta investigación es plantear un diagrama causal de un modelo teórico-hipotético de relaciones entre la bacteria biocontroladora Pasteuria sp., el nematodo fitoparásito Hirschmanniella spp. y el cultivo de arroz (Oryza sativa L.), como hospedero, cuyo propósito es tener una visión sobre la posible utilización de la Pasteuria sp. para el control de Hirschmanniella spp. en dicho cultivo. La metodología aplicada fue la de una investigación documental de fuentes primarias y secundarias y el análisis inductivo de las referencias consultadas. Se obtuvo como resultado un diagrama causal en el cual interactúan los tres entes biológicos en estudio, condicionados por la acción de otros factores bióticos y abióticos (edafoclimáticas principalmente), y factores relacionados al manejo integrado del cultivo.  En este último aspecto, la liberación de esporas de Pasteuria sp. tiene un lugar relevante. Se concluye que la bacteria biocontroladora Pasteuria sp., podría servir como agente regulador del nematodo fitoparásito Hirschmanniella spp. en el cultivo del arroz, siempre y cuando las condiciones inhernetes al cultivo,  el manejo integral del mismo y las condiciones edafoclimáticas, así lo permitan y favorezcan.
    Type of Medium: Online Resource
    ISSN: 2588-1000
    URL: Article
    DOI: 10.29018/issn.2588-1000vol5iss38.2021pp70-85
    Language: Unknown
    Publisher: Centro de Investigacion y Desarrollo Profesional - CIDEPRO
    Publication Date: 2021
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  • 2
    Online Resource
    Online Resource
    Shewanella septentrionalis sp. nov. and Shewanella holmiensis sp. nov., isolated from Baltic Sea water and sediments
    Microbiology Society ; 2023
    In:  International Journal of Systematic and Evolutionary Microbiology Vol. 73, No. 4 ( 2023-04-21)
    Details
    In: International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 73, No. 4 ( 2023-04-21)
    Abstract: Two bacterial strains, SP1W3 T and SP1S2-7 T , were isolated from samples of water and sediments collected in Vaxholm, a town located on the Stockholm archipelago in the Baltic Sea, in November 2021. The strains were identified as novel genomic species within the genus Shewanella , based upon comparative analysis of whole genome sequence data. Strain SP1W3 T (genome size, 5.20 Mbp; G+C content, 46.0 mol%), isolated from water, was determined to be most closely related to S. hafniensis ATCC-BAA 1207 T and S. baltica NCTC 10735 T , with digital DNA–DNA hybridization (dDDH) values of 61.7% and 60.4 %, respectively. Strain SP1S2-7 T (genome size, 4.26 Mbp; G+C content, 41.5 mol%), isolated from sediments, was observed to be most closely related to S. aestuarii JCM17801 T , with a pairwise dDDH value of 33.8 %. Polyphasic analyses of physiological and phenotypic characteristics, in addition to genomic analyses, confirmed that each of these two strains represent distinct, novel species within the genus Shewanella , for which the names Shewanella septentrionalis sp. nov. (type strain SP1W3 T =CCUG 76164 T =CECT 30651 T ) and Shewanella holmiensis sp. nov. (type strain SP1S2-7 T =CCUG 76165 T =CECT 30652 T ) are proposed.
    Type of Medium: Online Resource
    ISSN: 1466-5026 , 1466-5034
    URL: Article
    DOI: 10.1099/ijsem.0.005767
    Language: English
    Publisher: Microbiology Society
    Publication Date: 2023
    detail.hit.zdb_id: 215062-1
    detail.hit.zdb_id: 2056611-6
    SSG: 12
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  • 3
    Online Resource
    Online Resource
    Predicción de pérdidas de propagación para sistemas de Televisión Digital Terrestre
    Centro de Investigacion y Desarrollo Profesional - CIDEPRO ; 2019
    In:  Pro Sciences: Revista de Producción, Ciencias e Investigación Vol. 3, No. 29 ( 2019-12-27), p. 100-113
    Details
    In: Pro Sciences: Revista de Producción, Ciencias e Investigación, Centro de Investigacion y Desarrollo Profesional - CIDEPRO, Vol. 3, No. 29 ( 2019-12-27), p. 100-113
    Abstract: Este trabajo es una revisión de los diferentes modelos de predicción de pérdidas de propagación para sistemas de televisión digital terrestre (TDT), tanto para transmisión de datos, audio y video. Dicha revisión es de suma importancia dentro del diseño y montaje de infraestructura de redes de Telecomunicaciones para su planificación y dimensionamiento (PyD), la cual es indispensable antes de construir una red y no caer en el subdimensionamiento ni sobredimensionamiento de equipos e infraestructura. Dentro de esta investigación se comparará los diferentes modelos de medición y se concluirá el de mejor rendimiento en base al RMSE (Root Mean Square Error) para la predicción de pérdidas de propagación en los sistemas antes mencionados.
    Type of Medium: Online Resource
    ISSN: 2588-1000
    URL: Article
    DOI: 10.29018/issn.2588-1000vol3iss29.2019pp100-113
    Language: Unknown
    Publisher: Centro de Investigacion y Desarrollo Profesional - CIDEPRO
    Publication Date: 2019
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  • 4
    Online Resource
    Online Resource
    Molecular Epidemiology of Enteroaggregative Escherichia coli (EAEC) Isolates of Hospitalized Children from Bolivia Reveal High Heterogeneity and Multidrug-Resistance
    MDPI AG ; 2020
    In:  International Journal of Molecular Sciences Vol. 21, No. 24 ( 2020-12-15), p. 9543-
    Details
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 21, No. 24 ( 2020-12-15), p. 9543-
    Abstract: Enteroaggregative Escherichia coli (EAEC) is an emerging pathogen frequently associated with acute diarrhea in children and travelers to endemic regions. EAEC was found the most prevalent bacterial diarrheal pathogen from hospitalized Bolivian children less than five years of age with acute diarrhea from 2007 to 2010. Here, we further characterized the epidemiology of EAEC infection, virulence genes, and antimicrobial susceptibility of EAEC isolated from 414 diarrheal and 74 non-diarrheal cases. EAEC isolates were collected and subjected to a PCR-based virulence gene screening of seven virulence genes and a phenotypic resistance test to nine different antimicrobials. Our results showed that atypical EAEC (a-EAEC, AggR-negative) was significantly associated with diarrhea (OR, 1.62, 95% CI, 1.25 to 2.09, p 〈 0.001) in contrast to typical EAEC (t-EAEC, AggR-positive). EAEC infection was most prevalent among children between 7–12 months of age. The number of cases exhibited a biannual cycle with a major peak during the transition from warm to cold (April–June). Both typical and a-EAEC infections were graded as equally severe; however, t-EAEC harbored more virulence genes. aap, irp2 and pic were the most prevalent genes. Surprisingly, we detected 60% and 52.6% of multidrug resistance (MDR) EAEC among diarrheal and non-diarrheal cases. Resistance to ampicillin, sulfonamides, and tetracyclines was most common, being the corresponding antibiotics, the ones that are frequently used in Bolivia. Our work is the first study that provides comprehensive information on the high heterogenicity of virulence genes in t-EAEC and a- EAEC and the large prevalence of MDR EAEC in Bolivia.
    Type of Medium: Online Resource
    ISSN: 1422-0067
    URL: Article
    DOI: 10.3390/ijms21249543
    Language: English
    Publisher: MDPI AG
    Publication Date: 2020
    detail.hit.zdb_id: 2019364-6
    SSG: 12
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  • 5
    Online Resource
    Online Resource
    Identification of enterotoxigenic Escherichia coli (ETEC) clades with long-term global distribution
    Springer Science and Business Media LLC ; 2014
    In:  Nature Genetics Vol. 46, No. 12 ( 2014-12), p. 1321-1326
    Details
    In: Nature Genetics, Springer Science and Business Media LLC, Vol. 46, No. 12 ( 2014-12), p. 1321-1326
    Type of Medium: Online Resource
    ISSN: 1061-4036 , 1546-1718
    URL: Article
    DOI: 10.1038/ng.3145
    RVK:
    XA 10000
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2014
    detail.hit.zdb_id: 1494946-5
    SSG: 12
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  • 6
    Online Resource
    Online Resource
    Identification of new heat-stable (STa) enterotoxin allele variants produced by human enterotoxigenic Escherichia coli (ETEC)
    Elsevier BV ; 2016
    In:  International Journal of Medical Microbiology Vol. 306, No. 7 ( 2016-11), p. 586-594
    Details
    In: International Journal of Medical Microbiology, Elsevier BV, Vol. 306, No. 7 ( 2016-11), p. 586-594
    Type of Medium: Online Resource
    ISSN: 1438-4221
    URL: Article
    DOI: 10.1016/j.ijmm.2016.05.016
    RVK:
    XA 49980
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2016
    detail.hit.zdb_id: 2020515-6
    SSG: 12
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  • 7
    Online Resource
    Online Resource
    Repeated Isolation of Extended-Spectrum-β-Lactamase-Positive Escherichia coli Sequence Types 648 and 131 from Community Wastewater Indicates that Sewage Systems Are Important Sources of Emerging Clones of Antibiotic-Resistant Bacteria
    American Society for Microbiology ; 2019
    In:  Antimicrobial Agents and Chemotherapy Vol. 63, No. 9 ( 2019-09)
    Details
    In: Antimicrobial Agents and Chemotherapy, American Society for Microbiology, Vol. 63, No. 9 ( 2019-09)
    Abstract: Antibiotic resistance in bacteria is an emerging problem globally. Resistant bacteria are found in human and animal microbiota, as well as in the environment. Wastewater receives bacteria from all these sources and thus can provide a measurement of abundance and diversity of antibiotic-resistant bacteria circulating in communities. In this study, water samples were collected from a wastewater pump station in a Norwegian suburban community over a period of 15 months. A total of 45 daily samples were cultured and analyzed for the presence of Escherichia coli . Eighty E. coli -like colonies were collected from each daily sample and then phenotyped and analyzed for antibiotic resistance using the PhenePlate-AREB system. During the sampling period, two unique E. coli phenotypes with resistance to cefotaxime and cefpodoxime indicating carriage of extended-spectrum β-lactamases (ESBL) were observed repeatedly. Whole-genome sequencing of 15 representative isolates from the two phenotypes identified these as two distinct clones belonging to the two globally spread E. coli multilocus sequence types (STs) ST131 and ST648 and carrying bla CTX-M-15 . The number of ESBL-positive E. coli strains in the community wastewater pump station was 314 of 3,123 (10%) analyzed E. coli strains. Of the ESBL-positive isolates, 37% belonged to ST648, and 7% belonged to ST131. Repeated findings of CTX-M-15-positive ST648 and ST131 over time indicate that these STs are resident in the analyzed wastewater systems and/or circulate abundantly in the community.
    Type of Medium: Online Resource
    ISSN: 0066-4804 , 1098-6596
    URL: Article
    DOI: 10.1128/AAC.00823-19
    RVK:
    XA 24552
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 2019
    detail.hit.zdb_id: 1496156-8
    SSG: 12
    SSG: 15,3
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  • 8
    Online Resource
    Online Resource
    Analysis of Growth Phases of Enterotoxigenic Escherichia coli Reveals a Distinct Transition Phase before Entry into Early Stationary Phase with Shifts in Tryptophan, Fucose, and Putrescine Metabolism and Degradation of Neurotransmitter Precursors
    American Society for Microbiology ; 2022
    In:  Microbiology Spectrum Vol. 10, No. 4 ( 2022-08-31)
    Details
    In: Microbiology Spectrum, American Society for Microbiology, Vol. 10, No. 4 ( 2022-08-31)
    Abstract: Enterotoxigenic Escherichia coli (ETEC) is a major cause of diarrhea in children and adults in endemic areas. Gene regulation of ETEC during growth in vitro and in vivo needs to be further evaluated, and here we describe the full transcriptome and metabolome of ETEC during growth from mid-logarithmic growth to early stationary phase in rich medium (LB medium). We identified specific genes and pathways subjected to rapid transient alterations in gene expression and metabolite production during the transition from logarithmic to stationary growth. The transient phase was found to be different from the subsequent induction of early stationary phase-induced genes. The transient phase was characterized by the repression of genes and metabolites involved in organic substance transport. Genes involved in fucose and putrescine metabolism were upregulated, and genes involved in iron transport were repressed. Expression of toxins and colonization factors were not changed, suggesting retained virulence from mid-logarithmic to the start of the stationary phase. Metabolomic analyses showed that the transient phase was characterized by a drop of intracellular amino acids, e.g., l -tyrosine, l- tryptophan, l -phenylalanine, l -leucine, and l -glutamic acid, followed by increased levels at induction of stationary phase. A pathway enrichment analysis of the entire combined transcriptome and metabolome revealed that significant pathways during progression from logarithmic to early stationary phase are involved in the degradation of neurotransmitters aminobutyrate (GABA) and precursors of 5-hydroxytryptamine (serotonin). This work provides a comprehensive framework for further studies on transcriptional and metabolic regulation in pathogenic E. coli . IMPORTANCE We show that E. coli , exemplified by the pathogenic subspecies enterotoxigenic E. coli (ETEC), undergoes a stepwise transcriptional and metabolic transition into the stationary phase. At a specific entry point, E. coli induces activation and repression of specific pathways. This leads to a rapid decrease of intracellular levels of certain amino acids. The resulting metabolic activity leads to an intense but short peak of indole production, suggesting that this is the previously described “indole peak,” rapid decrease of intermediate molecules of bacterial neurotransmitters, increased putrescine and fucose uptake, increased glutathione levels, and decreased iron uptake. This specific transient shift in gene expression and metabolome is short-lived and disappears when bacteria enter the early stationary phase. We suggest that these changes mainly prepare bacteria for ceased growth, but based on the pathways involved, we could suggest that this transient phase substantially influences survival and virulence.
    Type of Medium: Online Resource
    ISSN: 2165-0497
    URL: Article
    DOI: 10.1128/spectrum.01755-21
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 2022
    detail.hit.zdb_id: 2807133-5
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  • 9
    Online Resource
    Online Resource
    Escherichia coli ST2797 Is Abundant in Wastewater and Might Be a Novel Emerging Extended-Spectrum Beta-Lactamase E. coli
    American Society for Microbiology ; 2023
    In:  Microbiology Spectrum Vol. 11, No. 4 ( 2023-08-17)
    Details
    In: Microbiology Spectrum, American Society for Microbiology, Vol. 11, No. 4 ( 2023-08-17)
    Abstract: The increasing prevalence of antibiotic-resistant bacteria is an emerging threat to global health. The analysis of antibiotic-resistant enterobacteria in wastewater can indicate the prevalence and spread of certain clonal groups of multiresistant bacteria. In a previous study of Escherichia coli that were isolated from a pump station in Norway over 15 months, we found a recurring E. coli clone that was resistant to trimethoprim, ampicillin, and tetracycline in 201 of 3,123 analyzed isolates (6.1%). 11 representative isolates were subjected to whole-genome sequencing and were found to belong to the MLST ST2797 E. coli clone with plasmids carrying resistance genes, including bla TEM-1B , sul2 , dfrA7 , and tetB. A phenotypic comparison of the ST2797 isolates with the uropathogenic ST131 and ST648 that were repeatedly identified in the same wastewater samples revealed that the ST2797 isolates exhibited a comparable capacity for temporal survival in wastewater, greater biofilm formation, and similar potential for the colonization of mammalian epithelial cells. ST2797 has been isolated from humans and has been found to carry extended spectrum β-lactamase (ESBL) genes in other studies, suggesting that this clonal type is an emerging ESBL E. coli . Collectively, these findings show that ST2797 was more ubiquitous in the studied wastewater than were the infamous ST131 and ST648 and that ST2797 may have similar abilities to survive in the environment and cause infections in humans. IMPORTANCE The incidence of drug-resistant bacteria found in the environment is increasing together with the levels of antibiotic-resistant bacteria that cause infections. The COVID-19 pandemic has shed new light on the importance of monitoring emerging threats and finding early warning systems. Therefore, to mitigate the antimicrobial resistance burden, the monitoring and early identification of antibiotic-resistant bacteria in hot spots, such as wastewater treatment plants, are required to combat the occurrence and spread of antibiotic-resistant bacteria. Here, we applied a PhenePlate system as a phenotypic screening method for genomic surveillance and discovered a dominant and persistent E. coli clone ST2797 with a multidrug resistance pattern and equivalent phenotypic characteristics to those of the major pandemic lineages, namely, ST131 and ST648, which frequently carry ESBL genes. This study highlights the continuous surveillance and report of multidrug resistant bacteria with the potential to spread in One Health settings.
    Type of Medium: Online Resource
    ISSN: 2165-0497
    URL: Article
    DOI: 10.1128/spectrum.04486-22
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 2023
    detail.hit.zdb_id: 2807133-5
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  • 10
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    Online Resource
    The LT1 and LT2 variants of the enterotoxigenic Escherichia coli (ETEC) heat-labile toxin (LT) are associated with major ETEC lineages
    Informa UK Limited ; 2016
    In:  Gut Microbes Vol. 7, No. 1 ( 2016-01-02), p. 75-81
    Details
    In: Gut Microbes, Informa UK Limited, Vol. 7, No. 1 ( 2016-01-02), p. 75-81
    Type of Medium: Online Resource
    ISSN: 1949-0976 , 1949-0984
    URL: Article
    DOI: 10.1080/19490976.2015.1127480
    Language: English
    Publisher: Informa UK Limited
    Publication Date: 2016
    detail.hit.zdb_id: 2575755-6
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