In:
Frontiers in Microbiology, Frontiers Media SA, Vol. 14 ( 2023-12-14)
Abstract:
Halophilic bacterial strains, designated SG2L-4 T , SB1M4, and SB2L-5, were isolated from jeotgal, a traditional Korean fermented food. Cells are Gram-stain-negative, aerobic, non-motile, rod-shaped, catalase-positive, and oxidase-negative. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain SG2L-4 T is closely related to Halomonas garicola KACC 18117 T with a similarity of 96.2%. The complete genome sequence of strain SG2L-4 T was 3,227,066 bp in size, with a genomic G + C content of 63.3 mol%. The average nucleotide identity and digital DNA–DNA hybridization values between strain SG2L-4 T and H. garicola KACC 18117 T were 90.5 and 40.7%, respectively. The optimal growth conditions for strain SG2L-4 T were temperatures between 30 and 37°C, a pH value of 7, and the presence of 10% (w/v) NaCl. The polar lipids identified included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unknown phospholipid, an unknown glycolipid, and an unknown polar lipid. The major cellular fatty acids were C 16:0 , summed features 8 (C 18:1 ω 6 c and/or C 18:1 ω 7 c ), C 19:0 cyclo ω 8 c , and summed features 3 (C 16:1 ω 6 c and/or C 16:1 ω 7 c ). The predominant respiratory quinone was ubiquinone with nine isoprene units (Q-9). Based on the phenotypic, genotypic, and chemotaxonomic results, strain SG2L-4 T represents a novel species within the genus Halomonas , for which the name Halomonas piscis sp. nov. is proposed. The type strain is SG2L-4 T (=KCTC 92842 T = JCM 35929 T ). Functional annotation of the genome of strain SG2L-4 T confirmed the presence of exopolysaccharide synthesis protein (ExoD) and capsular polysaccharide-related genes. Strain SG2L-4 T also exhibited positive results in Molisch’s test, indicating the presence of extracellular carbohydrates and exopolysaccharides (EPS) production. These findings provide valuable insights into the EPS-producing capabilities of H. piscis sp. nov. isolated from jeotgal, contributing to understanding its potential roles in food and biotechnological applications.
Type of Medium:
Online Resource
ISSN:
1664-302X
DOI:
10.3389/fmicb.2023.1303039
DOI:
10.3389/fmicb.2023.1303039.s001
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2023
detail.hit.zdb_id:
2587354-4
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