In:
Experimental Dermatology, Wiley, Vol. 23, No. 8 ( 2014-08), p. 600-602
Abstract:
Recently, we demonstrated that leucine‐rich glioma inactivated 3 ( LGI 3) is expressed in human skin. However, the effects of LGI 3 on melanocytes remain unknown. The present study demonstrated that LGI 3 can serve to stimulate melanogenesis without affecting cell viability. To determine the effects of LGI 3 on melanin synthesis, normal human melanocytes and Mel‐Ab cells were treated with recombinant LGI 3 and melanin content was measured. Our results showed that LGI 3 promoted melanin synthesis in both cell types. Moreover, upregulation of microphthalmia‐associated transcription factor ( MITF ) and tyrosinase was observed at both the mRNA and protein levels via RT ‐ PCR and Western blotting, respectively. Furthermore, immunohistochemical staining showed that the expression of LG I3 increased in the basal layer of melasma skin samples, whereas it decreased slightly in vitiligo samples. These results suggest that LG I3 may play a role as a melanogenic cytokine in human skin.
Type of Medium:
Online Resource
ISSN:
0906-6705
,
1600-0625
DOI:
10.1111/exd.2014.23.issue-8
Language:
English
Publisher:
Wiley
Publication Date:
2014
detail.hit.zdb_id:
2026228-0
Permalink