In:
Frontiers in Molecular Biosciences, Frontiers Media SA, Vol. 8 ( 2021-10-19)
Abstract:
Mdfi, an inhibitor of myogenic regulatory factors, is involved in myoblast myogenic development and muscle fiber type transformation. However, the regulatory network of Mdfi regulating myoblasts has not been revealed. In this study, we performed microRNAs (miRNAs)-seq on Mdfi overexpression (Mdfi-OE) and wild-type (WT) C2C12 cells to establish the regulatory networks. Comparative analyses of Mdfi-OE vs . WT identified 66 differentially expressed miRNAs (DEMs). Enrichment analysis of the target genes suggested that DEMs may be involved in myoblast differentiation and muscle fiber type transformation through MAPK, Wnt, PI3K-Akt, mTOR, and calcium signaling pathways. miRNA-mRNA interaction networks were suggested along with ten hub miRNAs and five hub genes. We also identified eight hub miRNAs and eleven hub genes in the networks of muscle fiber type transformation. Hub miRNAs mainly play key regulatory roles in muscle fiber type transformation through the PI3K-Akt, MAPK, cAMP, and calcium signaling pathways. Particularly, the three hub miRNAs (miR-335-3p, miR-494-3p, and miR-709) may be involved in both myogenic differentiation and muscle fiber type transformation. These hub miRNAs and genes might serve as candidate biomarkers for the treatment of muscle- and metabolic-related diseases.
Type of Medium:
Online Resource
ISSN:
2296-889X
DOI:
10.3389/fmolb.2021.675993
DOI:
10.3389/fmolb.2021.675993.s001
DOI:
10.3389/fmolb.2021.675993.s002
DOI:
10.3389/fmolb.2021.675993.s003
DOI:
10.3389/fmolb.2021.675993.s004
DOI:
10.3389/fmolb.2021.675993.s005
DOI:
10.3389/fmolb.2021.675993.s006
DOI:
10.3389/fmolb.2021.675993.s007
DOI:
10.3389/fmolb.2021.675993.s008
DOI:
10.3389/fmolb.2021.675993.s009
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2021
detail.hit.zdb_id:
2814330-9
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