In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 62, No. Pt_12 ( 2012-12-01), p. 2927-2933
Abstract:
The bacterial strain LH2-2 T was isolated from freshwater of Longhu Lake, a slightly alkaline lake (pH 8.8) in north-east China. Cells of strain LH2-2 T were Gram-staining-negative, non-spore-forming rods, 0.3–0.5 µm wide and 2.0–4.0 µm long. Cells were motile by means of a single polar flagellum. The strain was strictly aerobic and heterotrophic and oxidase- and catalase-positive. Growth occurred at 0–36 °C (optimum, 26–34 °C), pH 6.5–11 (optimum, pH 8.0–8.6) and in the presence of 0–2 % (w/v) NaCl (optimum, 1 %). Strain LH2-2 T contained Q-8 as the major respiratory quinone. The major fatty acids were summed feature 3 (C 16 : 1 ω7 c and/or iso-C 15 : 0 2-OH; 21.9 %), C 17 : 1 ω8 c (18.9 %), C 18 : 1 ω7 c (16.4 %) and C 16 : 0 (12.7 %) after growth on marine agar 2216. The DNA G+C content was 47 mol% ( T m ). The 16S rRNA gene and a conserved portion of the gyrB gene were sequenced and used for phylogenetic analyses. Phylogenetic trees based on 16S rRNA gene and gyrB sequences showed that strain LH2-2 T was associated with the genus Rheinheimera and closely related to the type strains of Rheinheimera species, and showed the highest 16S rRNA gene sequence similarity to Rheinheimera pacifica KMM 1406 T (97.4 %), R. aquimaris SW-353 T (97.1 %) and R. chironomi K19414 T (96.5 %). The DNA–DNA relatedness of strain LH2-2 T to R. pacifica NBRC 103167 T , R. aquimaris JCM 14331 T and R. chironomi LMG 23818 T was 39, 31 and 23 %, respectively. Based on these results, it is concluded that strain LH2-2 T represents a novel species of the genus Rheinheimera , for which the name Rheinheimera longhuensis sp. nov. is proposed. The type strain is LH2-2 T ( = CGMCC 1.7003 T = NBRC 105632 T ). An emended description of the genus Rheinheimera is also provided.
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.036020-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2012
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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