In:
PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 6 ( 2022-6-28), p. e0270708-
Abstract:
In order to develop an appropriate method for high-throughput detection of avian metapneumovirus, a quadruple real-time reverse-transcription polymerase chain reaction assay was established with four pairs of specific primers and four specific probes based on the G or M gene of aMPV-A, aMPV-B, aMPV-C and aMPV-D. Its specificity and sensitivity were evaluated, and clinical samples were tested by the method. The results showed that all the four subgroups of avian metapneumovirus can be detected in the quadruple real-time RT-PCR assay simultaneously, with a detection limit of 100–1000 cRNA copies/reaction. The other common poultry viruses were negative. In the avian clinical sample detection, 39 out of 1920 clinical samples collected from 8 provinces were positive. Compared with published RT-PCR assays, the κ value of the quadruple real-time RT-PCR assay in 1920 avian clinical samples was 1.000 ( P 〈 0.001). The established method could be used for the rapid detection of the four subgroups of avian metapneumovirus with high specificity and high sensitivity.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0270708
DOI:
10.1371/journal.pone.0270708.g001
DOI:
10.1371/journal.pone.0270708.g002
DOI:
10.1371/journal.pone.0270708.t001
DOI:
10.1371/journal.pone.0270708.t002
DOI:
10.1371/journal.pone.0270708.t003
DOI:
10.1371/journal.pone.0270708.t004
DOI:
10.1371/journal.pone.0270708.t005
DOI:
10.1371/journal.pone.0270708.t006
DOI:
10.1371/journal.pone.0270708.s001
DOI:
10.1371/journal.pone.0270708.s002
DOI:
10.1371/journal.pone.0270708.s003
DOI:
10.1371/journal.pone.0270708.s004
DOI:
10.1371/journal.pone.0270708.s005
DOI:
10.1371/journal.pone.0270708.s006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2267670-3
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