In:
Molecular Biology of the Cell, American Society for Cell Biology (ASCB), Vol. 9, No. 4 ( 1998-04), p. 749-757
Abstract:
Gene targeting techniques and early mouse embryos have been used to produce immortalized fibroblasts genetically deficient in phospholipase C (PLC)-γ1, a ubiquitous tyrosine kinase substrate.Plcg1 −/− embryos die at embryonic day 9; however, cells derived from these embryos proliferate as well as cells from Plcg1 +/+ embryos. The null cells do grow to a higher saturation density in serum-containing media, as their capacity to spread out is decreased compared with that of wild-type cells. In terms of epidermal growth factor receptor activation and internalization, or growth factor induction of mitogen-activated protein kinase, c-fos, or DNA synthesis in quiescent cells, PLcg1 −/− cells respond equivalently to PLcg1 +/+ cells. Also, null cells are able to migrate effectively in a wounded monolayer. Therefore, immortalized fibroblasts do not require PLC-γ1 for many responses to growth factors.
Type of Medium:
Online Resource
ISSN:
1059-1524
,
1939-4586
Language:
English
Publisher:
American Society for Cell Biology (ASCB)
Publication Date:
1998
detail.hit.zdb_id:
1474922-1
SSG:
12
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