In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 71, No. 7 ( 2021-07-02)
Abstract:
Taxonomic positions of six isolates, which were recovered from two different environments in Jeju, Republic of Korea, were examined by a polyphasic analysis. Cells of the isolates were Gram-reaction-negative, facultatively anaerobic, motile and rod-shaped and showed growth at 4–30 °C, pH 4.0–9.0 and with 0–6 (w/v) NaCl. In phylogenomic analysis based on 92 single-copy core genes, it was shown that the isolates belonged to the genus Rahnella and formed three distinct sublines within the genus. The isolates shared 16S rRNA gene sequence similarities of 97.9–100 % with one another. The isolates contained ubiquinone-8 was as the major isoprenoid quinone. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and an unidentified aminophospholipid. The predominant fatty acids were C 16 : 0 and C 17 : 0 cyclo. The G+C content of their genomic DNA was 52.8–53.1 %. Average nucleotide identity and digital DNA–DNA hybridization values supported that strains SAP-17 T and Lac-M11 T represented two new species of the genus Rahnella , whereas strain SAP-10 was a strain of Rahnella victoriana . Based on the results obtained here, Rahnella laticis sp. nov. (type strain SAP-17 T =KCTC 72960 T =NBRC 114723 T =CCM 9079 T ) and Rahnella contaminans sp. nov. (type strain Lac-M11 T =KACC 21743 T =NBRC 114406 T ) are proposed. Also, an emended description of the genus Rahnella is given on the basis of our physiological and chemotaxonomic results.
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijsem.0.004893
Language:
English
Publisher:
Microbiology Society
Publication Date:
2021
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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