In:
PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 2 ( 2022-2-8), p. e0263684-
Abstract:
Since the SARS-CoV-2 infection was identified in December 2019, SARS-CoV-2 infection has rapidly spread worldwide and has become a significant pandemic disease. In addition, human death and serious health problem caused by SARS-CoV-2 infection, the socio-economic impact has been very serious. Here, we describe the development of the viral vector vaccine, which is the receptor-binding domain (RBD) of SARS-CoV-2 expressed on the surface of Newcastle disease virus (LVP-K1-RBD19). The RBD protein concentrations on the viral surface were measured by the sandwich ELISA method. 10 6.7 TCID 50 /ml of LVP-K1-RBD19 has a 0.17 μg of RBD protein. Optical density (OD) values of mouse sera inoculated with 10 μg of RBD protein expressed on the surface of LVP-K1-RBD19 generated 1.78-fold higher RBD-specific antibody titers than mice inoculated with 10 μg RBD protein with alum at 28 dpi. Moreover, mice inoculated with 10 μg of RBD protein expressed on the surface of LVP-K1-RBD19 virus showed more than 80% neutralization at 1:256 against the SARS-CoV-2 pseudovirus. These results demonstrated that inactivated LVP-K1-RBD19 virus produces neutralizing antibodies against SARS-CoV-2 in a short period and could be elect protective immunity in humans and LVP-K1-RBD19 will be a good candidate for the COVID-19 vaccine.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0263684
DOI:
10.1371/journal.pone.0263684.g001
DOI:
10.1371/journal.pone.0263684.g002
DOI:
10.1371/journal.pone.0263684.g003
DOI:
10.1371/journal.pone.0263684.g004
DOI:
10.1371/journal.pone.0263684.g005
DOI:
10.1371/journal.pone.0263684.t001
DOI:
10.1371/journal.pone.0263684.t002
DOI:
10.1371/journal.pone.0263684.t003
DOI:
10.1371/journal.pone.0263684.s001
DOI:
10.1371/journal.pone.0263684.s002
DOI:
10.1371/journal.pone.0263684.s003
DOI:
10.1371/journal.pone.0263684.s004
DOI:
10.1371/journal.pone.0263684.s005
DOI:
10.1371/journal.pone.0263684.s006
DOI:
10.1371/journal.pone.0263684.s007
DOI:
10.1371/journal.pone.0263684.s008
DOI:
10.1371/journal.pone.0263684.s009
DOI:
10.1371/journal.pone.0263684.s010
DOI:
10.1371/journal.pone.0263684.s011
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2267670-3
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