In:
Animal Bioscience, Asian Australasian Association of Animal Production Societies, Vol. 36, No. 9 ( 2023-09-01), p. 1357-1366
Abstract:
Objective: This study aimed to identify the single-nucleotide polymorphisms (SNPs) in the dual-specificity phosphatase 8 ( 〈 i 〉 DUSP8 〈 /i 〉 ) and insulin-like growth factor 2 ( 〈 i 〉 IGF2 〈 /i 〉 ) genes and to explore their effects on inosine-5′-monophosphate (IMP), inosine, and hypoxanthine contents in Korean native chicken -red-brown line (KNC-R Line).Methods: A total sample of 284 (males, n = 127; females n = 157) and 230 (males, n = 106; females, n = 124) aged of 10 weeks old KNC-R line was used for genotyping of 〈 i 〉 DUSP8 〈 /i 〉 and 〈 i 〉 IGF2 〈 /i 〉 genes, respectively. One SNP (rs313443014 C 〉 T) in 〈 i 〉 DUSP8 〈 /i 〉 gene and two SNPs (rs315806609A/G and rs313810945T/C) in IGF2 gene were used for genotyping by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and KASP methods, respectively. The Two-way analysis of variance of the R program was used to associate DUSP8 and IGF2 genotypes with nucleotide contents in KNC-R chickens.Results: The DUSP8 (rs313443014 C 〉 T) was polymorphic in KNC-R line and showed three genotypes: CC, CT, and TT. The 〈 i 〉 IGF2 〈 /i 〉 gene (rs315806609A/G and rs313810945T/C) was also polymorphic and had three genotypes per SNP, including GG, AG, and AA for the SNP rs315806609A/G and genotypes: CC, CT, and TT for the SNP rs313810945T/C. Association resulted into a strong significant association (p 〈 0.01) with IMP, inosine, and hypoxanthine. Moreover, the significant effect of sex (p 〈 0.05) on nucleotide content was also observed.Conclusion: The SNPs in the 〈 i 〉 DUSP8 〈 /i 〉 and 〈 i 〉 IGF2 〈 /i 〉 genes might be used as genetic markers in the selection and production of chickens with highly flavored meat.
Type of Medium:
Online Resource
ISSN:
2765-0189
,
2765-0235
Language:
English
Publisher:
Asian Australasian Association of Animal Production Societies
Publication Date:
2023
detail.hit.zdb_id:
3055169-9
Permalink