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  • 1
    Online Resource
    Online Resource
    Distribusi dan Kelimpahan Kista Pyrodinium bahamense di Perairan Rawan Marak Alga Berbahaya di Indonesia
    National Research and Innovation Agency ; 2021
    In:  OLDI (Oseanologi dan Limnologi di Indonesia) Vol. 6, No. 1 ( 2021-04-28), p. 37-
    Details
    In: OLDI (Oseanologi dan Limnologi di Indonesia), National Research and Innovation Agency, Vol. 6, No. 1 ( 2021-04-28), p. 37-
    Abstract: 〈 strong 〉 Distribution and abundance of 〈 em 〉 Pyrodinium bahamense 〈 /em 〉 cyst in the harmful algal blooms risk waters in Indonesia. 〈 /strong 〉 〈 em 〉 Pyrodinium bahamense 〈 /em 〉 as Harmful Algal Blooms (HABs) is one of the rising environmental problems in the coastal areas of Southeast Asia, particularly in Indonesia. Cyst bank formed after the blooms of 〈 em 〉 P. bahamense 〈 /em 〉 is a potential source for the future blooming events. Therefore, an effort to describe the distribution and abundance of 〈 em 〉 P. bahamense 〈 /em 〉 cyst banks in Indonesian coastal waters is necessary as a part of the mitigation strategy against the negative impacts of those toxic dinoflagellate blooms. This study was done as a desk study, which included a literature study, reanalysed of previous research data, and reanalysed of preserved samples or stored raw samples. Data and samples used in this study were collected and analysed from part of past researches in Lampung Bay, Jakarta Bay, Cirebon coastal waters, and Ambon Bay. This study also included an analysis to determine the 〈 em 〉 P. Bahamense 〈 /em 〉 HABs risk level in the studied areas based on the cyst density and distribution data. Results showed a low density of 〈 em 〉 P. bahamense 〈 /em 〉 cyst in Lampung Bay and Jakarta Bay, with cyst density & lt;50 cysts.g 〈 sup 〉 -1 〈 /sup 〉 wet sediment. In contrast, the bottom sediments of Ambon Bay and Cirebon coastal waters contained high cyst density, which reached & gt;1,000 cysts.g 〈 sup 〉 -1 〈 /sup 〉 wet sediment. Based on those data, the 〈 em 〉 P. Bahamense 〈 /em 〉 HABs risk in Jakarta Bay and Lampung Bay would be generally much lower compared to Ambon Bay and Cirebon waters. The existence of cyst banks in those coastal waters may indicate  a possibility of future blooms of 〈 em 〉 P. bahamense 〈 /em 〉 .
    Type of Medium: Online Resource
    ISSN: 2477-328X
    URL: Article
    DOI: 10.14203/oldi.2021.v6i1.337
    Language: Unknown
    Publisher: National Research and Innovation Agency
    Publication Date: 2021
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  • 2
    Online Resource
    Online Resource
    High prevalence of hepatitis B virus genotype C/C1 in the Minangkabau ethnic group in Indonesia
    Springer Science and Business Media LLC ; 2013
    In:  Virology Journal Vol. 10, No. 1 ( 2013-12)
    Details
    In: Virology Journal, Springer Science and Business Media LLC, Vol. 10, No. 1 ( 2013-12)
    Abstract: The Minangkabau is one of the major ethnic groups in Indonesia. Previous studies with a limited number of samples have shown a different prevalence of HBV/C in the Minangkabau compared to the Indonesian population in general. The aim of this study was to assess the HBV genotype distribution pattern and the prevalence of pre-S, T1753V and A1762T/G1764A mutations among the Minangkabau HBV carriers. The samples were collected from Padang, West Sumatera and from western Java. Mixed primers for specific genotypes were used to determine the HBV genotype. Pre-S or S genes were amplified, sequenced and aligned with reference sequences from GenBank to derive a phylogenetic tree for subgenotyping. Pre-S genes were also analyzed for mutations. The basal core promoter (BCP) region was amplified and directly sequenced to analyze T1753V and A1762T/G1764A mutations. Results The predominant HBV genotype among the Minangkabau HBV carriers (n=117) was C (72.6%) followed by B (24.8%) and co-infection with B and C (2.6%). The prevalence of pre-S mutations, including both the pre-S deletion and pre-S2 start codon mutation, was 41.0%, and the T1753V and A1762T/G1764A mutations were found in 51.9% and 71.2% respectively. HBV/C1 was the predominant HBV subgenotype in the Minangkabau HBV carriers, and was found in 66.2%, followed by B3, B7, C8, B2, B9, C2, and C10 (18.3%, 7.0%, 2.8%, 1.4%, 1.4%, 1.4%, and 1.4% respectively). From samples that were found to be co-infected with HBV B and C, two samples were successfully cloned and subgenotyped, including one with mixed subgenotypes of B3 and C1, and another one with mixed subgenotypes of B7, C1, putative intergenotypic of B/A, and C/A. Furthermore, three samples from donors of non-Minangkabau ethnicity from Padang were found to be infected with an intragenotypic recombination form, including a putative recombinant of B8/B3 and B9/B7. Conclusion HBV/C with subgenotype C1 was the predominant HBV genotype among HBV carriers of Minangkabau ethnicity. The prevalence of pre-S, A1762T/G1764A, and T1753V mutations was higher among the Minangkabau compared to Indonesian HBV carriers in general.
    Type of Medium: Online Resource
    ISSN: 1743-422X
    URL: Article
    DOI: 10.1186/1743-422X-10-27
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2013
    detail.hit.zdb_id: 2160640-7
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