In:
Frontiers in Plant Science, Frontiers Media SA, Vol. 14 ( 2023-9-6)
Abstract:
All known photosynthetic cyanobacteria carry a cytochrome c 6 protein that acts transferring electrons from cytochrome b 6 f complex to photosystem I, in photosynthesis, or cytochrome c oxidase, in respiration. In most of the cyanobacteria, at least one homologue to cytochrome c 6 is found, the so-called cytochrome c 6B or cytochrome c 6C . However, the function of these cytochrome c 6 -like proteins is still unknown. Recently, it has been proposed a common origin of these proteins as well as the reclassification of the cytochrome c 6C group as c 6B , renaming the new joint group as cytochrome c 6BC . Another homologue to cytochrome c 6 has not been classified yet, the formerly called cytochrome c 6-3 , which is present in the heterocyst-forming filamentous cyanobacteria Nostoc sp. PCC 7119. In this work, we propose the inclusion of this group as an independent group in the genealogy of cytochrome c 6 -like proteins with significant differences from cytochrome c 6 and cytochrome c 6BC , with the proposed name cytochrome c 6D . To support this proposal, new data about phylogeny, genome localisation and functional properties of cytochrome c 6 -like proteins is provided. Also, we have analysed the interaction of cytochrome c 6 -like proteins with cytochrome f by isothermal titration calorimetry and by molecular docking, concluding that c 6 -like proteins could interact with cytochrome b 6 f complex in a similar fashion as cytochrome c 6 . Finally, we have analysed the reactivity of cytochrome c 6 -like proteins with membranes enriched in terminal oxidases of cyanobacteria by oxygen uptake experiments, concluding that cytochrome c 6D is able to react with the specific copper-oxidase of the heterocysts, the cytochrome c oxidase 2.
Type of Medium:
Online Resource
ISSN:
1664-462X
DOI:
10.3389/fpls.2023.1227492
DOI:
10.3389/fpls.2023.1227492.s001
DOI:
10.3389/fpls.2023.1227492.s002
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2023
detail.hit.zdb_id:
2687947-5
detail.hit.zdb_id:
2613694-6
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